(Linear) (Six-base) MAPSORT of: KIEE3062.seq  Check: 588  from: 1  to: 651



KIEE3062.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:08  ..

AflIII A'CryG_T

Cuts at:      0     306     651 
   Size:        306     345
 
ApoI r'AATT_y

Cuts at:      0     548     651 
   Size:        548     103
 
AvaI C'yCGr_G

Cuts at:      0     100     651 
   Size:        100     551
 
BbvCI CC'TCA_GC

Cuts at:      0     468     651 
   Size:        468     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     651 
   Size:        181     470
 
Bpu10I CC'TnA_GC

Cuts at:      0     468     651 
   Size:        468     183
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     133     163     651 
   Size:        133      30     488

  Fragments arranged by size: 

                 488     133      30
 
BseYI C'CCAG_C

Cuts at:      0     566     651 
   Size:        566      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     651 
   Size:         58     593
 
BsmI GAATG_Cn'

Cuts at:      0     606     646     651 
   Size:        606      40       5

  Fragments arranged by size: 

                 606      40       5
 
BsrBI CCG'CTC

Cuts at:      0     219     651 
   Size:        219     432
 
BsrGI T'GTAC_A

Cuts at:      0     342     651 
   Size:        342     309
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      65     478     651 
   Size:         65     413     173

  Fragments arranged by size: 

                 413     173      65
 
BstYI r'GATC_y

Cuts at:      0      14     651 
   Size:         14     637
 
BtgI C'CryG_G

Cuts at:      0      53     651 
   Size:         53     598
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     130     651 
   Size:        130     521
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      16     651 
   Size:         16     635
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     651 
   Size:        181     470
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165     651 
   Size:        133      32     486

  Fragments arranged by size: 

                 486     133      32
 
MslI CAynn'nnrTG

Cuts at:      0     383     651 
   Size:        383     268
 
NspI r_CATG'y

Cuts at:      0     310     651 
   Size:        310     341
 
PasI CC'CwG_GG

Cuts at:      0      87     651 
   Size:         87     564
 
PciI A'CATG_T

Cuts at:      0     306     651 
   Size:        306     345
 
SfcI C'TryA_G

Cuts at:      0     190     651 
   Size:        190     461
 
SmaI CCC'GGG

Cuts at:      0     102     651 
   Size:        102     549
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     100     651 
   Size:        100     551
 
TatI w'GTAC_w

Cuts at:      0     342     651 
   Size:        342     309
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     135     651 
   Size:        135     516
 
XmaI C'CCGG_G

Cuts at:      0     100     651 
   Size:        100     551
 

 Enzymes that do cut:

   AflIII     ApoI     AvaI    BbvCI     BpmI   Bpu10I    BsaXI    BseYI
     BsgI     BsmI    BsrBI    BsrGI   BstAPI    BstYI     BtgI     DrdI
     EciI  Eco57MI    Hin4I     MslI     NspI     PasI     PciI     SfcI
     SmaI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI