(Linear) (Six-base) MAPSORT of: KIEE3060.seq  Check: 1588  from: 1  to: 642



KIEE3060.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:08  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     511     642 
   Size:        511     131
 
ApaLI G'TGCA_C

Cuts at:      0     501     555     642 
   Size:        501      54      87

  Fragments arranged by size: 

                 501      87      54
 
ApoI r'AATT_y

Cuts at:      0      64     642 
   Size:         64     578
 
BanI G'GyrC_C

Cuts at:      0     508     642 
   Size:        508     134
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     370     404     642 
   Size:        370      34     238

  Fragments arranged by size: 

                 370     238      34
 
BlpI GC'TnA_GC

Cuts at:      0      74     642 
   Size:         74     568
 
Bme1580I G_kGCm'C

Cuts at:      0     246     505     559     642 
   Size:        246     259      54      83

  Fragments arranged by size: 

                 259     246      83      54
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     642 
   Size:         43     599
 
Bpu10I CC'TnA_GC

Cuts at:      0      48     642 
   Size:         48     594
 
BsaBI GATnn'nnATC

Cuts at:      0     298     642 
   Size:        298     344
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     352     642 
   Size:        352     290
 
BseYI C'CCAG_C

Cuts at:      0      29     642 
   Size:         29     613
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     377     642 
   Size:        297      80     265

  Fragments arranged by size: 

                 297     265      80
 
BsiHKAI G_wGCw'C

Cuts at:      0     505     559     642 
   Size:        505      54      83

  Fragments arranged by size: 

                 505      83      54
 
Bsp1286I G_dGCh'C

Cuts at:      0     246     505     559     642 
   Size:        246     259      54      83

  Fragments arranged by size: 

                 259     246      83      54
 
BstYI r'GATC_y

Cuts at:      0     420     642 
   Size:        420     222
 
EarI CTCTTCn'nnn_

Cuts at:      0     128     642 
   Size:        128     514
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     642 
   Size:         43     599
 
MfeI C'AATT_G

Cuts at:      0     200     642 
   Size:        200     442
 
MspA1I CmG'CkG

Cuts at:      0     186     208     642 
   Size:        186      22     434

  Fragments arranged by size: 

                 434     186      22
 
NspI r_CATG'y

Cuts at:      0     236     642 
   Size:        236     406
 
PfoI T'CCnGG_A

Cuts at:      0      59     642 
   Size:         59     583
 
PstI C_TGCA'G

Cuts at:      0     185     642 
   Size:        185     457
 
PvuII CAG'CTG

Cuts at:      0     186     208     642 
   Size:        186      22     434

  Fragments arranged by size: 

                 434     186      22
 
SfcI C'TryA_G

Cuts at:      0     181     209     642 
   Size:        181      28     433

  Fragments arranged by size: 

                 433     181      28
 
StyI C'CwwG_G

Cuts at:      0     540     642 
   Size:        540     102
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     549     581     642 
   Size:        549      32      61

  Fragments arranged by size: 

                 549      61      32
 

 Enzymes that do cut:

     AlfI    ApaLI     ApoI     BanI     BcgI     BlpI Bme1580I     BpmI
   Bpu10I    BsaBI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BstYI
     EarI  Eco57MI     MfeI   MspA1I     NspI     PfoI     PstI    PvuII
     SfcI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI