Restriction map for
pFN21AE3059
(Linear) (Six-base) MAPSORT of: KIEE3059.seq Check: 8215 from: 1 to: 639
KIEE3059.orf
With 182 enzymes: SgfI *
March 22, 2012 16:07 ..
AfeI AGC'GCT
Cuts at: 0 453 639
Size: 453 186
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 466 498 639
Size: 466 32 141
Fragments arranged by size:
466 141 32
AlwNI CAG_nnn'CTG
Cuts at: 0 55 639
Size: 55 584
ApoI r'AATT_y
Cuts at: 0 28 639
Size: 28 611
AvaI C'yCGr_G
Cuts at: 0 140 639
Size: 140 499
BamHI G'GATC_C
Cuts at: 0 136 639
Size: 136 503
BanII G_rGCy'C
Cuts at: 0 252 553 639
Size: 252 301 86
Fragments arranged by size:
301 252 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 169 639
Size: 169 470
BbvCI CC'TCA_GC
Cuts at: 0 623 639
Size: 623 16
Bme1580I G_kGCm'C
Cuts at: 0 498 609 639
Size: 498 111 30
Fragments arranged by size:
498 111 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 421 463 639
Size: 266 155 42 176
Fragments arranged by size:
266 176 155 42
Bpu10I CC'TnA_GC
Cuts at: 0 380 623 639
Size: 380 243 16
Fragments arranged by size:
380 243 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 639
Size: 472 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 439 639
Size: 1 438 200
Fragments arranged by size:
438 200 1
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 466 496 639
Size: 466 30 143
Fragments arranged by size:
466 143 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 639
Size: 490 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 639
Size: 68 571
BsmI GAATG_Cn'
Cuts at: 0 557 639
Size: 557 82
Bsp1286I G_dGCh'C
Cuts at: 0 252 498 553 609 639
Size: 252 246 55 56 30
Fragments arranged by size:
252 246 56 55 30
BsrBI CCG'CTC
Cuts at: 0 204 384 639
Size: 204 180 255
Fragments arranged by size:
255 204 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 342 639
Size: 342 297
BstYI r'GATC_y
Cuts at: 0 136 639
Size: 136 503
BtgI C'CryG_G
Cuts at: 0 614 639
Size: 614 25
BtsI GCAGTG_nn'
Cuts at: 0 51 639
Size: 51 588
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 107 138 142 173 639
Size: 107 31 4 31 466
Fragments arranged by size:
466 107 31 31 4
EaeI y'GGCC_r
Cuts at: 0 195 326 639
Size: 195 131 313
Fragments arranged by size:
313 195 131
EarI CTCTTCn'nnn_
Cuts at: 0 28 639
Size: 28 611
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 571 639
Size: 571 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 421 463 639
Size: 266 155 42 176
Fragments arranged by size:
266 176 155 42
EcoO109I rG'GnC_Cy
Cuts at: 0 369 639
Size: 369 270
HaeII r_GCGC'y
Cuts at: 0 455 639
Size: 455 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 438 470 639
Size: 121 32 285 32 169
Fragments arranged by size:
285 169 121 32 32
HincII GTy'rAC
Cuts at: 0 150 639
Size: 150 489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 639
Size: 134 505
MscI TGG'CCA
Cuts at: 0 197 328 639
Size: 197 131 311
Fragments arranged by size:
311 197 131
PflMI CCAn_nnn'nTGG
Cuts at: 0 615 639
Size: 615 24
PfoI T'CCnGG_A
Cuts at: 0 398 440 639
Size: 398 42 199
Fragments arranged by size:
398 199 42
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 421 453 466 498 639
Size: 79 32 310 32 13 32 141
Fragments arranged by size:
310 141 79 32 32 32 13
PpuMI rG'GwC_Cy
Cuts at: 0 369 639
Size: 369 270
SfcI C'TryA_G
Cuts at: 0 175 639
Size: 175 464
SmaI CCC'GGG
Cuts at: 0 142 639
Size: 142 497
SmlI C'TyrA_G
Cuts at: 0 487 639
Size: 487 152
StuI AGG'CCT
Cuts at: 0 406 639
Size: 406 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 145 200 639
Size: 145 55 439
Fragments arranged by size:
439 145 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 639
Size: 128 32 479
Fragments arranged by size:
479 128 32
XmaI C'CCGG_G
Cuts at: 0 140 639
Size: 140 499
XmnI GAAnn'nnTTC
Cuts at: 0 94 639
Size: 94 545
Enzymes that do cut:
AfeI AloI AlwNI ApoI AvaI BamHI BanII BbsI
BbvCI Bme1580I BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BsgI BsmI Bsp1286I BsrBI BstXI BstYI BtgI BtsI
CspCI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I
HincII MmeI MscI PflMI PfoI PpiI PpuMI SfcI
SmaI SmlI StuI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
Tth111I XbaI XcmI XhoI ZraI