(Linear) (Six-base) MAPSORT of: KIEE3058.seq  Check: 2307  from: 1  to: 639



KIEE3058.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     639 
   Size:        446     193
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      47     206     449     639 
   Size:         47     159     243     190

  Fragments arranged by size: 

                 243     190     159      47
 
BlpI GC'TnA_GC

Cuts at:      0     307     639 
   Size:        307     332
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      46      76     639 
   Size:         46      30     563

  Fragments arranged by size: 

                 563      46      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67      70     639 
   Size:         67       3     569

  Fragments arranged by size: 

                 569      67       3
 
BseYI C'CCAG_C

Cuts at:      0     187     639 
   Size:        187     452
 
BsrBI CCG'CTC

Cuts at:      0      21     639 
   Size:         21     618
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     178     639 
   Size:        178     461
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     485     491     639 
   Size:         44     441       6     148

  Fragments arranged by size: 

                 441     148      44       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28     639 
   Size:         28     611
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     639 
   Size:        446     193
 
EcoO109I rG'GnC_Cy

Cuts at:      0     212     639 
   Size:        212     427
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     306     338     639 
   Size:         91      32     183      32     301

  Fragments arranged by size: 

                 301     183      91      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     437     639 
   Size:        437     202
 
MspA1I CmG'CkG

Cuts at:      0     176     639 
   Size:        176     463
 
NspI r_CATG'y

Cuts at:      0     247     639 
   Size:        247     392
 
PpuMI rG'GwC_Cy

Cuts at:      0     212     639 
   Size:        212     427
 
PvuII CAG'CTG

Cuts at:      0     176     639 
   Size:        176     463
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     165     639 
   Size:        165     474
 
XmnI GAAnn'nnTTC

Cuts at:      0     500     639 
   Size:        500     139
 

 Enzymes that do cut:

     AcuI     BbsI     BlpI    BsaXI    BseRI    BseYI    BsrBI     DrdI
     EarI     EciI  Eco57MI EcoO109I    Hin4I     MslI   MspA1I     NspI
    PpuMI    PvuII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI