(Linear) (Six-base) MAPSORT of: KIEE3056.seq  Check: 4183  from: 1  to: 636



KIEE3056.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     636 
   Size:        452     184
 
AhdI GACnn_n'nnGTC

Cuts at:      0     235     636 
   Size:        235     401
 
ApoI r'AATT_y

Cuts at:      0     483     636 
   Size:        483     153
 
BanII G_rGCy'C

Cuts at:      0     449     636 
   Size:        449     187
 
BbvCI CC'TCA_GC

Cuts at:      0     145     636 
   Size:        145     491
 
BmtI G_CTAG'C

Cuts at:      0     565     636 
   Size:        565      71
 
Bpu10I CC'TnA_GC

Cuts at:      0     145     152     470     636 
   Size:        145       7     318     166

  Fragments arranged by size: 

                 318     166     145       7
 
BsaWI w'CCGG_w

Cuts at:      0     109     636 
   Size:        109     527
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94      97     636 
   Size:         94       3     539

  Fragments arranged by size: 

                 539      94       3
 
BsmI GAATG_Cn'

Cuts at:      0     375     636 
   Size:        375     261
 
Bsp1286I G_dGCh'C

Cuts at:      0     449     636 
   Size:        449     187
 
BspEI T'CCGG_A

Cuts at:      0     109     636 
   Size:        109     527
 
BsrDI GCAATG_nn'

Cuts at:      0     163     636 
   Size:        163     473
 
BstYI r'GATC_y

Cuts at:      0     106     546     636 
   Size:        106     440      90

  Fragments arranged by size: 

                 440     106      90
 
Bsu36I CC'TnA_GG

Cuts at:      0     364     636 
   Size:        364     272
 
DraI TTT'AAA

Cuts at:      0     482     636 
   Size:        482     154
 
EaeI y'GGCC_r

Cuts at:      0      47     636 
   Size:         47     589
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     636 
   Size:        452     184
 
EcoRV GAT'ATC

Cuts at:      0       8     636 
   Size:          8     628
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     221     253     636 
   Size:        221      32     383

  Fragments arranged by size: 

                 383     221      32
 
HincII GTy'rAC

Cuts at:      0      72     636 
   Size:         72     564
 
MfeI C'AATT_G

Cuts at:      0     455     617     636 
   Size:        455     162      19

  Fragments arranged by size: 

                 455     162      19
 
MscI TGG'CCA

Cuts at:      0      49     636 
   Size:         49     587
 
NheI G'CTAG_C

Cuts at:      0     561     636 
   Size:        561      75
 
SwaI ATTT'AAAT

Cuts at:      0     482     636 
   Size:        482     154
 

 Enzymes that do cut:

     AcuI     AhdI     ApoI    BanII    BbvCI     BmtI   Bpu10I    BsaWI
    BseRI     BsmI Bsp1286I    BspEI    BsrDI    BstYI   Bsu36I     DraI
     EaeI  Eco57MI    EcoRV    Hin4I   HincII     MfeI     MscI     NheI
     SwaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI