(Linear) (Six-base) MAPSORT of: KIEE3055.seq  Check: 1435  from: 1  to: 624



KIEE3055.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:32  ..

AccI GT'mk_AC

Cuts at:      0     159     624 
   Size:        159     465
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     249     258     624 
   Size:        238      11       9     366

  Fragments arranged by size: 

                 366     238      11       9
 
AlwNI CAG_nnn'CTG

Cuts at:      0     485     624 
   Size:        485     139
 
ApoI r'AATT_y

Cuts at:      0     322     624 
   Size:        322     302
 
AvaI C'yCGr_G

Cuts at:      0     618     624 
   Size:        618       6
 
BamHI G'GATC_C

Cuts at:      0     449     624 
   Size:        449     175
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     576     624 
   Size:        576      48
 
BbvCI CC'TCA_GC

Cuts at:      0     206     624 
   Size:        206     418
 
BglII A'GATC_T

Cuts at:      0     260     624 
   Size:        260     364
 
Bpu10I CC'TnA_GC

Cuts at:      0     206     624 
   Size:        206     418
 
BstYI r'GATC_y

Cuts at:      0     260     449     624 
   Size:        260     189     175

  Fragments arranged by size: 

                 260     189     175
 
BtsI GCAGTG_nn'

Cuts at:      0     160     624 
   Size:        160     464
 
DraI TTT'AAA

Cuts at:      0     116     624 
   Size:        116     508
 
EaeI y'GGCC_r

Cuts at:      0     233     624 
   Size:        233     391
 
EarI CTCTTCn'nnn_

Cuts at:      0     277     624 
   Size:        277     347
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     249     258     624 
   Size:        238      11       9     366

  Fragments arranged by size: 

                 366     238      11       9
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      33     416     624 
   Size:         33     383     208

  Fragments arranged by size: 

                 383     208      33
 
MscI TGG'CCA

Cuts at:      0     235     624 
   Size:        235     389
 
SfcI C'TryA_G

Cuts at:      0     477     602     624 
   Size:        477     125      22

  Fragments arranged by size: 

                 477     125      22
 
SspI AAT'ATT

Cuts at:      0     285     624 
   Size:        285     339
 
TatI w'GTAC_w

Cuts at:      0     437     624 
   Size:        437     187
 
XbaI T'CTAG_A

Cuts at:      0      63     624 
   Size:         63     561
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AvaI    BamHI     BbsI    BbvCI
    BglII   Bpu10I    BstYI     BtsI     DraI     EaeI     EarI  Eco57MI
     FalI     MscI     SfcI     SspI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI    BanII     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI