(Linear) (Six-base) MAPSORT of: KIEE3046.seq  Check: 3920  from: 1  to: 555



KIEE3046.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:32  ..

AclI AA'CG_TT

Cuts at:      0     543     555 
   Size:        543      12
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     275     555 
   Size:         43     232     280

  Fragments arranged by size: 

                 280     232      43
 
AflIII A'CryG_T

Cuts at:      0     151     555 
   Size:        151     404
 
ApoI r'AATT_y

Cuts at:      0     238     453     555 
   Size:        238     215     102

  Fragments arranged by size: 

                 238     215     102
 
BanI G'GyrC_C

Cuts at:      0     279     314     555 
   Size:        279      35     241

  Fragments arranged by size: 

                 279     241      35
 
BanII G_rGCy'C

Cuts at:      0     393     555 
   Size:        393     162
 
BglII A'GATC_T

Cuts at:      0     308     555 
   Size:        308     247
 
BmtI G_CTAG'C

Cuts at:      0      13     555 
   Size:         13     542
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43     555 
   Size:         43     512
 
BsaWI w'CCGG_w

Cuts at:      0     515     555 
   Size:        515      40
 
BseYI C'CCAG_C

Cuts at:      0     386     555 
   Size:        386     169
 
Bsp1286I G_dGCh'C

Cuts at:      0     393     555 
   Size:        393     162
 
BspEI T'CCGG_A

Cuts at:      0     515     555 
   Size:        515      40
 
BsrGI T'GTAC_A

Cuts at:      0     148     555 
   Size:        148     407
 
BstYI r'GATC_y

Cuts at:      0     308     555 
   Size:        308     247
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     275     555 
   Size:         43     232     280

  Fragments arranged by size: 

                 280     232      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0      47     555 
   Size:         47     508
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     528     555 
   Size:        528      27
 
HindIII A'AGCT_T

Cuts at:      0     181     555 
   Size:        181     374
 
MfeI C'AATT_G

Cuts at:      0     371     555 
   Size:        371     184
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     555 
   Size:         95     460
 
NheI G'CTAG_C

Cuts at:      0       9     555 
   Size:          9     546
 
NspI r_CATG'y

Cuts at:      0     155     555 
   Size:        155     400
 
PciI A'CATG_T

Cuts at:      0     151     555 
   Size:        151     404
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     311     555 
   Size:        311     244
 
PfoI T'CCnGG_A

Cuts at:      0      49     555 
   Size:         49     506
 
PpuMI rG'GwC_Cy

Cuts at:      0      47     555 
   Size:         47     508
 
PshAI GACnn'nnGTC

Cuts at:      0      35     555 
   Size:         35     520
 
PstI C_TGCA'G

Cuts at:      0     486     555 
   Size:        486      69
 
SanDI GG'GwC_CC

Cuts at:      0      47     555 
   Size:         47     508
 
SfcI C'TryA_G

Cuts at:      0     482     555 
   Size:        482      73
 
SspI AAT'ATT

Cuts at:      0     474     555 
   Size:        474      81
 
TatI w'GTAC_w

Cuts at:      0     148     555 
   Size:        148     407
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     ApoI     BanI    BanII    BglII     BmtI
     BsaI    BsaWI    BseYI Bsp1286I    BspEI    BsrGI    BstYI  Eco57MI
 EcoO109I     FalI  HindIII     MfeI     MmeI     NheI     NspI     PciI
    PflMI     PfoI    PpuMI    PshAI     PstI    SanDI     SfcI     SspI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI