Restriction map for
pFN21AE3045
(Linear) (Six-base) MAPSORT of: KIEE3045.seq Check: 1132 from: 1 to: 552
KIEE3045.orf
With 182 enzymes: SgfI *
March 22, 2012 16:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 286 552
Size: 86 200 266
Fragments arranged by size:
266 200 86
AflII C'TTAA_G
Cuts at: 0 295 552
Size: 295 257
AflIII A'CryG_T
Cuts at: 0 511 513 552
Size: 511 2 39
Fragments arranged by size:
511 39 2
AleI CACnn'nnGTG
Cuts at: 0 514 552
Size: 514 38
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 217 552
Size: 217 335
ApoI r'AATT_y
Cuts at: 0 442 552
Size: 442 110
AvaI C'yCGr_G
Cuts at: 0 205 552
Size: 205 347
BanI G'GyrC_C
Cuts at: 0 91 552
Size: 91 461
BanII G_rGCy'C
Cuts at: 0 30 552
Size: 30 522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 260 294 522 552
Size: 260 34 228 30
Fragments arranged by size:
260 228 34 30
Bme1580I G_kGCm'C
Cuts at: 0 196 552
Size: 196 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 241 552
Size: 147 94 311
Fragments arranged by size:
311 147 94
BsaAI yAC'GTr
Cuts at: 0 514 552
Size: 514 38
BsaWI w'CCGG_w
Cuts at: 0 392 552
Size: 392 160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 226 552
Size: 28 198 326
Fragments arranged by size:
326 198 28
BseYI C'CCAG_C
Cuts at: 0 116 149 287 552
Size: 116 33 138 265
Fragments arranged by size:
265 138 116 33
Bsp1286I G_dGCh'C
Cuts at: 0 30 196 552
Size: 30 166 356
Fragments arranged by size:
356 166 30
BspEI T'CCGG_A
Cuts at: 0 392 552
Size: 392 160
BsrFI r'CCGG_y
Cuts at: 0 57 552
Size: 57 495
BssSI C'ACGA_G
Cuts at: 0 310 552
Size: 310 242
BstXI CCAn_nnnn'nTGG
Cuts at: 0 157 546 552
Size: 157 389 6
Fragments arranged by size:
389 157 6
BtsI GCAGTG_nn'
Cuts at: 0 194 529 552
Size: 194 335 23
Fragments arranged by size:
335 194 23
ClaI AT'CG_AT
Cuts at: 0 542 552
Size: 542 10
EaeI y'GGCC_r
Cuts at: 0 357 552
Size: 357 195
EarI CTCTTCn'nnn_
Cuts at: 0 496 552
Size: 496 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 147 241 286 552
Size: 86 61 94 45 266
Fragments arranged by size:
266 94 86 61 45
EcoO109I rG'GnC_Cy
Cuts at: 0 47 83 237 552
Size: 47 36 154 315
Fragments arranged by size:
315 154 47 36
MslI CAynn'nnrTG
Cuts at: 0 199 451 514 552
Size: 199 252 63 38
Fragments arranged by size:
252 199 63 38
NaeI GCC'GGC
Cuts at: 0 59 552
Size: 59 493
NgoMIV G'CCGG_C
Cuts at: 0 57 552
Size: 57 495
NspI r_CATG'y
Cuts at: 0 247 552
Size: 247 305
PfoI T'CCnGG_A
Cuts at: 0 163 552
Size: 163 389
PmlI CAC'GTG
Cuts at: 0 514 552
Size: 514 38
PpuMI rG'GwC_Cy
Cuts at: 0 47 83 552
Size: 47 36 469
Fragments arranged by size:
469 47 36
SanDI GG'GwC_CC
Cuts at: 0 47 83 552
Size: 47 36 469
Fragments arranged by size:
469 47 36
SfcI C'TryA_G
Cuts at: 0 481 552
Size: 481 71
SmaI CCC'GGG
Cuts at: 0 207 552
Size: 207 345
SmlI C'TyrA_G
Cuts at: 0 295 552
Size: 295 257
StyI C'CwwG_G
Cuts at: 0 87 183 552
Size: 87 96 369
Fragments arranged by size:
369 96 87
Tth111I GACn'n_nGTC
Cuts at: 0 141 552
Size: 141 411
XmaI C'CCGG_G
Cuts at: 0 205 552
Size: 205 347
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI ApoI AvaI BanI
BanII BcgI Bme1580I BpmI BsaAI BsaWI BseRI BseYI
Bsp1286I BspEI BsrFI BssSI BstXI BtsI ClaI EaeI
EarI Eco57MI EcoO109I MslI NaeI NgoMIV NspI PfoI
PmlI PpuMI SanDI SfcI SmaI SmlI StyI Tth111I
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI XbaI XcmI
XhoI XmnI ZraI