(Linear) (Six-base) MAPSORT of: KIEE3044.seq  Check: 800  from: 1  to: 549



KIEE3044.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     549 
   Size:        368     181
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     484     516     549 
   Size:        484      32      33

  Fragments arranged by size: 

                 484      33      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     181     549 
   Size:        181     368
 
ApoI r'AATT_y

Cuts at:      0     161     549 
   Size:        161     388
 
BanII G_rGCy'C

Cuts at:      0     399     549 
   Size:        399     150
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     374     549 
   Size:        374     175
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     111     145     549 
   Size:        111      34     404

  Fragments arranged by size: 

                 404     111      34
 
BglII A'GATC_T

Cuts at:      0     204     549 
   Size:        204     345
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      83     549 
   Size:         83     466
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     389     549 
   Size:        389     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     549 
   Size:         82     467
 
BsiHKAI G_wGCw'C

Cuts at:      0     399     549 
   Size:        399     150
 
Bsp1286I G_dGCh'C

Cuts at:      0     399     549 
   Size:        399     150
 
BsrGI T'GTAC_A

Cuts at:      0     529     549 
   Size:        529      20
 
BstYI r'GATC_y

Cuts at:      0     204     354     549 
   Size:        204     150     195

  Fragments arranged by size: 

                 204     195     150
 
EaeI y'GGCC_r

Cuts at:      0     222     549 
   Size:        222     327
 
EarI CTCTTCn'nnn_

Cuts at:      0     147     304     549 
   Size:        147     157     245

  Fragments arranged by size: 

                 245     157     147
 
EcoICRI GAG'CTC

Cuts at:      0     397     549 
   Size:        397     152
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     549 
   Size:        368     181
 
EcoRV GAT'ATC

Cuts at:      0     276     549 
   Size:        276     273
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     364     396     549 
   Size:        364      32     153

  Fragments arranged by size: 

                 364     153      32
 
SacI G_AGCT'C

Cuts at:      0     399     549 
   Size:        399     150
 
SmlI C'TyrA_G

Cuts at:      0     404     549 
   Size:        404     145
 
StyI C'CwwG_G

Cuts at:      0     225     549 
   Size:        225     324
 
TatI w'GTAC_w

Cuts at:      0     529     549 
   Size:        529      20
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     335     367     549 
   Size:        335      32     182

  Fragments arranged by size: 

                 335     182      32
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI    BanII     BbsI     BcgI    BglII
     BmrI    BpuEI     BsgI  BsiHKAI Bsp1286I    BsrGI    BstYI     EaeI
     EarI  EcoICRI  Eco57MI    EcoRV    Hin4I     SacI     SmlI     StyI
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI