(Linear) (Six-base) MAPSORT of: KIEE3043.seq  Check: 9953  from: 1  to: 549



KIEE3043.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     542     549 
   Size:        377     165       7

  Fragments arranged by size: 

                 377     165       7
 
ApaI G_GGCC'C

Cuts at:      0      49     549 
   Size:         49     500
 
AvaI C'yCGr_G

Cuts at:      0      42     549 
   Size:         42     507
 
BanII G_rGCy'C

Cuts at:      0      49     549 
   Size:         49     500
 
Bme1580I G_kGCm'C

Cuts at:      0      49     549 
   Size:         49     500
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     549 
   Size:        106     443
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     549 
   Size:         49     500
 
BseYI C'CCAG_C

Cuts at:      0     488     549 
   Size:        488      61
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     549 
   Size:         49     500
 
BsrBI CCG'CTC

Cuts at:      0      13      68     549 
   Size:         13      55     481

  Fragments arranged by size: 

                 481      55      13
 
BsrDI GCAATG_nn'

Cuts at:      0     103     549 
   Size:        103     446
 
BsrFI r'CCGG_y

Cuts at:      0      95     549 
   Size:         95     454
 
BsrGI T'GTAC_A

Cuts at:      0      17     549 
   Size:         17     532
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     138     549 
   Size:        138     411
 
BtsI GCAGTG_nn'

Cuts at:      0     234     549 
   Size:        234     315
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     542     549 
   Size:        377     165       7

  Fragments arranged by size: 

                 377     165       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39      46      58     549 
   Size:         39       7      12     491

  Fragments arranged by size: 

                 491      39      12       7
 
MfeI C'AATT_G

Cuts at:      0     410     549 
   Size:        410     139
 
MspA1I CmG'CkG

Cuts at:      0     135     397     492     549 
   Size:        135     262      95      57

  Fragments arranged by size: 

                 262     135      95      57
 
NaeI GCC'GGC

Cuts at:      0      97     549 
   Size:         97     452
 
NgoMIV G'CCGG_C

Cuts at:      0      95     549 
   Size:         95     454
 
PpuMI rG'GwC_Cy

Cuts at:      0      39     549 
   Size:         39     510
 
PshAI GACnn'nnGTC

Cuts at:      0     483     549 
   Size:        483      66
 
PspOMI G'GGCC_C

Cuts at:      0      45     549 
   Size:         45     504
 
PstI C_TGCA'G

Cuts at:      0     229     549 
   Size:        229     320
 
PvuII CAG'CTG

Cuts at:      0     135     397     492     549 
   Size:        135     262      95      57

  Fragments arranged by size: 

                 262     135      95      57
 
SfcI C'TryA_G

Cuts at:      0     225     549 
   Size:        225     324
 
SmaI CCC'GGG

Cuts at:      0      44     549 
   Size:         44     505
 
SmlI C'TyrA_G

Cuts at:      0     121     549 
   Size:        121     428
 
TatI w'GTAC_w

Cuts at:      0      17     184     549 
   Size:         17     167     365

  Fragments arranged by size: 

                 365     167      17
 
XmaI C'CCGG_G

Cuts at:      0      42     549 
   Size:         42     507
 

 Enzymes that do cut:

     AcuI     ApaI     AvaI    BanII Bme1580I    BpuEI    BseRI    BseYI
 Bsp1286I    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI     BtsI  Eco57MI
 EcoO109I     MfeI   MspA1I     NaeI   NgoMIV    PpuMI    PshAI   PspOMI
     PstI    PvuII     SfcI     SmaI     SmlI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI