(Linear) (Six-base) MAPSORT of: KIEE3037.seq  Check: 8219  from: 1  to: 507



KIEE3037.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     507 
   Size:        252     255
 
AflIII A'CryG_T

Cuts at:      0     176     327     507 
   Size:        176     151     180

  Fragments arranged by size: 

                 180     176     151
 
BanI G'GyrC_C

Cuts at:      0      90     507 
   Size:         90     417
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     173     353     375     507 
   Size:        173     180      22     132

  Fragments arranged by size: 

                 180     173     132      22
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     234     507 
   Size:        234     273
 
BclI T'GATC_A

Cuts at:      0     147     507 
   Size:        147     360
 
BfrBI ATG'CAT

Cuts at:      0     190     507 
   Size:        190     317
 
Bme1580I G_kGCm'C

Cuts at:      0      93     507 
   Size:         93     414
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     507 
   Size:        328     179
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     474     507 
   Size:        474      33
 
BseYI C'CCAG_C

Cuts at:      0      20     507 
   Size:         20     487
 
BsiHKAI G_wGCw'C

Cuts at:      0      35      56     507 
   Size:         35      21     451

  Fragments arranged by size: 

                 451      35      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      35      56      93     507 
   Size:         35      21      37     414

  Fragments arranged by size: 

                 414      37      35      21
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      97     507 
   Size:         97     410
 
BtsI GCAGTG_nn'

Cuts at:      0     471     507 
   Size:        471      36
 
EarI CTCTTCn'nnn_

Cuts at:      0      47      61     488     507 
   Size:         47      14     427      19

  Fragments arranged by size: 

                 427      47      19      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     507 
   Size:        252     255
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     460     492     507 
   Size:        460      32      15

  Fragments arranged by size: 

                 460      32      15
 
HincII GTy'rAC

Cuts at:      0     286     507 
   Size:        286     221
 
MslI CAynn'nnrTG

Cuts at:      0     175     245     374     507 
   Size:        175      70     129     133

  Fragments arranged by size: 

                 175     133     129      70
 
NsiI A_TGCA'T

Cuts at:      0     192     507 
   Size:        192     315
 
NspI r_CATG'y

Cuts at:      0     180     244     507 
   Size:        180      64     263

  Fragments arranged by size: 

                 263     180      64
 
PciI A'CATG_T

Cuts at:      0     176     507 
   Size:        176     331
 
SapI GCTCTTCn'nnn_

Cuts at:      0      61     507 
   Size:         61     446
 
SmlI C'TyrA_G

Cuts at:      0     307     507 
   Size:        307     200
 
SphI G_CATG'C

Cuts at:      0     244     507 
   Size:        244     263
 
SspI AAT'ATT

Cuts at:      0     433     507 
   Size:        433      74
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     100     507 
   Size:        100     407
 

 Enzymes that do cut:

     AcuI   AflIII     BanI     BbsI    BciVI     BclI    BfrBI Bme1580I
    BpuEI    BseRI    BseYI  BsiHKAI Bsp1286I   BstAPI     BtsI     EarI
  Eco57MI    Hin4I   HincII     MslI     NsiI     NspI     PciI     SapI
     SmlI     SphI     SspI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI