(Linear) (Six-base) MAPSORT of: KIEE3036.seq  Check: 7989  from: 1  to: 507



KIEE3036.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     266     507 
   Size:        266     241
 
AflIII A'CryG_T

Cuts at:      0     174     507 
   Size:        174     333
 
BamHI G'GATC_C

Cuts at:      0     486     507 
   Size:        486      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     170     507 
   Size:        170     337
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     127     507 
   Size:        127     380
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     507 
   Size:        167     340
 
BsaAI yAC'GTr

Cuts at:      0     175     507 
   Size:        175     332
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      27     507 
   Size:         27     480
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     507 
   Size:        126     381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     266     507 
   Size:        266     241
 
BsrFI r'CCGG_y

Cuts at:      0      34     507 
   Size:         34     473
 
BstYI r'GATC_y

Cuts at:      0     486     507 
   Size:        486      21
 
Bsu36I CC'TnA_GG

Cuts at:      0     480     507 
   Size:        480      27
 
BtgI C'CryG_G

Cuts at:      0       5     507 
   Size:          5     502
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42     507 
   Size:         42     465
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     227     259     507 
   Size:        227      32     248

  Fragments arranged by size: 

                 248     227      32
 
MspA1I CmG'CkG

Cuts at:      0       7     507 
   Size:          7     500
 
NaeI GCC'GGC

Cuts at:      0      36     507 
   Size:         36     471
 
NgoMIV G'CCGG_C

Cuts at:      0      34     507 
   Size:         34     473
 
PmlI CAC'GTG

Cuts at:      0     175     507 
   Size:        175     332
 
PpuMI rG'GwC_Cy

Cuts at:      0      42     507 
   Size:         42     465
 
PshAI GACnn'nnGTC

Cuts at:      0     260     507 
   Size:        260     247
 
PstI C_TGCA'G

Cuts at:      0     277     424     507 
   Size:        277     147      83

  Fragments arranged by size: 

                 277     147      83
 
SacII CC_GC'GG

Cuts at:      0       8     507 
   Size:          8     499
 
SanDI GG'GwC_CC

Cuts at:      0      42     507 
   Size:         42     465
 
SfcI C'TryA_G

Cuts at:      0      79     273     420     507 
   Size:         79     194     147      87

  Fragments arranged by size: 

                 194     147      87      79
 
SmlI C'TyrA_G

Cuts at:      0     182     507 
   Size:        182     325
 
TatI w'GTAC_w

Cuts at:      0      94     507 
   Size:         94     413
 

 Enzymes that do cut:

     AarI   AflIII    BamHI     BbsI    BciVI    BpuEI    BsaAI    BseRI
     BsgI    BspMI    BsrFI    BstYI   Bsu36I     BtgI EcoO109I    Hin4I
   MspA1I     NaeI   NgoMIV     PmlI    PpuMI    PshAI     PstI    SacII
    SanDI     SfcI     SmlI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI