Restriction map for
pFN21AE3035
(Linear) (Six-base) MAPSORT of: KIEE3035.seq Check: 3468 from: 1 to: 504
KIEE3035.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 231 504
Size: 231 273
AvaI C'yCGr_G
Cuts at: 0 403 504
Size: 403 101
BanI G'GyrC_C
Cuts at: 0 82 504
Size: 82 422
BanII G_rGCy'C
Cuts at: 0 404 504
Size: 404 100
BbeI G_GCGC'C
Cuts at: 0 86 504
Size: 86 418
BglI GCCn_nnn'nGGC
Cuts at: 0 234 504
Size: 234 270
Bme1580I G_kGCm'C
Cuts at: 0 412 504
Size: 412 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 504
Size: 147 357
Bpu10I CC'TnA_GC
Cuts at: 0 484 504
Size: 484 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 421 504
Size: 187 234 83
Fragments arranged by size:
234 187 83
BsaAI yAC'GTr
Cuts at: 0 327 504
Size: 327 177
BsaHI Gr'CG_yC
Cuts at: 0 83 504
Size: 83 421
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 328 358 391 421 504
Size: 328 30 33 30 83
Fragments arranged by size:
328 83 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 352 355 504
Size: 265 87 3 149
Fragments arranged by size:
265 149 87 3
BseYI C'CCAG_C
Cuts at: 0 374 504
Size: 374 130
BsiEI CG_ry'CG
Cuts at: 0 76 504
Size: 76 428
BsiHKAI G_wGCw'C
Cuts at: 0 404 504
Size: 404 100
Bsp1286I G_dGCh'C
Cuts at: 0 404 412 504
Size: 404 8 92
Fragments arranged by size:
404 92 8
BsrFI r'CCGG_y
Cuts at: 0 71 75 464 504
Size: 71 4 389 40
Fragments arranged by size:
389 71 40 4
BssSI C'ACGA_G
Cuts at: 0 394 504
Size: 394 110
BstAPI GCAn_nnn'nTGC
Cuts at: 0 231 504
Size: 231 273
BstEII G'GTnAC_C
Cuts at: 0 183 504
Size: 183 321
BtgI C'CryG_G
Cuts at: 0 319 504
Size: 319 185
BtsI GCAGTG_nn'
Cuts at: 0 466 504
Size: 466 38
EaeI y'GGCC_r
Cuts at: 0 73 294 504
Size: 73 221 210
Fragments arranged by size:
221 210 73
EagI C'GGCC_G
Cuts at: 0 73 504
Size: 73 431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 504
Size: 70 434
EcoICRI GAG'CTC
Cuts at: 0 402 504
Size: 402 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 504
Size: 147 357
EcoO109I rG'GnC_Cy
Cuts at: 0 174 504
Size: 174 330
HaeII r_GCGC'y
Cuts at: 0 86 504
Size: 86 418
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 504
Size: 328 32 144
Fragments arranged by size:
328 144 32
HindIII A'AGCT_T
Cuts at: 0 10 504
Size: 10 494
KasI G'GCGC_C
Cuts at: 0 82 504
Size: 82 422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 504
Size: 456 48
MslI CAynn'nnrTG
Cuts at: 0 475 504
Size: 475 29
MspA1I CmG'CkG
Cuts at: 0 231 321 504
Size: 231 90 183
Fragments arranged by size:
231 183 90
NaeI GCC'GGC
Cuts at: 0 77 504
Size: 77 427
NarI GG'CG_CC
Cuts at: 0 83 504
Size: 83 421
NgoMIV G'CCGG_C
Cuts at: 0 75 504
Size: 75 429
NspI r_CATG'y
Cuts at: 0 474 504
Size: 474 30
PmlI CAC'GTG
Cuts at: 0 327 504
Size: 327 177
PspXI vC'TCGA_Gb
Cuts at: 0 403 504
Size: 403 101
SacI G_AGCT'C
Cuts at: 0 404 504
Size: 404 100
SacII CC_GC'GG
Cuts at: 0 322 504
Size: 322 182
SfoI GGC'GCC
Cuts at: 0 84 504
Size: 84 420
SmlI C'TyrA_G
Cuts at: 0 202 403 436 504
Size: 202 201 33 68
Fragments arranged by size:
202 201 68 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 504
Size: 391 32 81
Fragments arranged by size:
391 81 32
XhoI C'TCGA_G
Cuts at: 0 403 504
Size: 403 101
Enzymes that do cut:
AlwNI AvaI BanI BanII BbeI BglI Bme1580I BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BsrFI BssSI BstAPI BstEII BtgI BtsI
EaeI EagI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HindIII KasI MmeI MslI MspA1I NaeI NarI NgoMIV
NspI PmlI PspXI SacI SacII SfoI SmlI TstI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI BsaI BsaBI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KpnI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XmaI XmnI ZraI