(Linear) (Six-base) MAPSORT of: KIEE3034.seq  Check: 4657  from: 1  to: 498



KIEE3034.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:32  ..

AccI GT'mk_AC

Cuts at:      0      10     498 
   Size:         10     488
 
AlwNI CAG_nnn'CTG

Cuts at:      0     456     498 
   Size:        456      42
 
BanII G_rGCy'C

Cuts at:      0     204     498 
   Size:        204     294
 
BglI GCCn_nnn'nGGC

Cuts at:      0     162     498 
   Size:        162     336
 
BglII A'GATC_T

Cuts at:      0     468     498 
   Size:        468      30
 
Bme1580I G_kGCm'C

Cuts at:      0     465     498 
   Size:        465      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     354     369     498 
   Size:        354      15     129

  Fragments arranged by size: 

                 354     129      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     498 
   Size:        493       5
 
BsaBI GATnn'nnATC

Cuts at:      0     145     498 
   Size:        145     353
 
BsmI GAATG_Cn'

Cuts at:      0     248     498 
   Size:        248     250
 
Bsp1286I G_dGCh'C

Cuts at:      0     204     465     498 
   Size:        204     261      33

  Fragments arranged by size: 

                 261     204      33
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     456     498 
   Size:        456      42
 
BstYI r'GATC_y

Cuts at:      0     468     498 
   Size:        468      30
 
BtgI C'CryG_G

Cuts at:      0     160     498 
   Size:        160     338
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27      59     498 
   Size:         27      32     439

  Fragments arranged by size: 

                 439      32      27
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     498 
   Size:        218     280
 
MspA1I CmG'CkG

Cuts at:      0     303     498 
   Size:        303     195
 
NcoI C'CATG_G

Cuts at:      0     160     498 
   Size:        160     338
 
PstI C_TGCA'G

Cuts at:      0     284     498 
   Size:        284     214
 
PvuII CAG'CTG

Cuts at:      0     303     498 
   Size:        303     195
 
SfcI C'TryA_G

Cuts at:      0      11     102     280     498 
   Size:         11      91     178     218

  Fragments arranged by size: 

                 218     178      91      11
 
SmlI C'TyrA_G

Cuts at:      0     472     498 
   Size:        472      26
 
SspI AAT'ATT

Cuts at:      0     392     498 
   Size:        392     106
 
StyI C'CwwG_G

Cuts at:      0     160     498 
   Size:        160     338
 
Tth111I GACn'n_nGTC

Cuts at:      0      27     498 
   Size:         27     471
 

 Enzymes that do cut:

     AccI    AlwNI    BanII     BglI    BglII Bme1580I     BmrI    BpuEI
    BsaBI     BsmI Bsp1286I   BstAPI    BstYI     BtgI    Hin4I     MmeI
   MspA1I     NcoI     PstI    PvuII     SfcI     SmlI     SspI     StyI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI
    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI