(Linear) (Six-base) MAPSORT of: KIEE3031.seq  Check: 815  from: 1  to: 468



KIEE3031.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AfeI AGC'GCT

Cuts at:      0     250     468 
   Size:        250     218
 
AvaI C'yCGr_G

Cuts at:      0      11     344     442     468 
   Size:         11     333      98      26

  Fragments arranged by size: 

                 333      98      26      11
 
BanI G'GyrC_C

Cuts at:      0      29     438     468 
   Size:         29     409      30

  Fragments arranged by size: 

                 409      30      29
 
BanII G_rGCy'C

Cuts at:      0      59     468 
   Size:         59     409
 
BglI GCCn_nnn'nGGC

Cuts at:      0     178     468 
   Size:        178     290
 
BglII A'GATC_T

Cuts at:      0     419     468 
   Size:        419      49
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     231     468 
   Size:        231     237
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     214     468 
   Size:         43     171     254

  Fragments arranged by size: 

                 254     171      43
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     327     468 
   Size:        327     141
 
BsaWI w'CCGG_w

Cuts at:      0     425     468 
   Size:        425      43
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     468 
   Size:         52     416
 
BsiEI CG_ry'CG

Cuts at:      0     258     468 
   Size:        258     210
 
BsiHKAI G_wGCw'C

Cuts at:      0      12      59     468 
   Size:         12      47     409

  Fragments arranged by size: 

                 409      47      12
 
Bsp1286I G_dGCh'C

Cuts at:      0      12      59     468 
   Size:         12      47     409

  Fragments arranged by size: 

                 409      47      12
 
BsrDI GCAATG_nn'

Cuts at:      0     359     468 
   Size:        359     109
 
BsrFI r'CCGG_y

Cuts at:      0      26     468 
   Size:         26     442
 
BstYI r'GATC_y

Cuts at:      0     419     468 
   Size:        419      49
 
Bsu36I CC'TnA_GG

Cuts at:      0      35     468 
   Size:         35     433
 
BtgI C'CryG_G

Cuts at:      0     182     468 
   Size:        182     286
 
EaeI y'GGCC_r

Cuts at:      0     179     468 
   Size:        179     289
 
EarI CTCTTCn'nnn_

Cuts at:      0     226     468 
   Size:        226     242
 
EcoICRI GAG'CTC

Cuts at:      0      57     468 
   Size:         57     411
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     214     468 
   Size:         43     171     254

  Fragments arranged by size: 

                 254     171      43
 
HaeII r_GCGC'y

Cuts at:      0     190     252     468 
   Size:        190      62     216

  Fragments arranged by size: 

                 216     190      62
 
MspA1I CmG'CkG

Cuts at:      0     184     334     468 
   Size:        184     150     134

  Fragments arranged by size: 

                 184     150     134
 
PvuII CAG'CTG

Cuts at:      0     334     468 
   Size:        334     134
 
SacI G_AGCT'C

Cuts at:      0      59     468 
   Size:         59     409
 
SacII CC_GC'GG

Cuts at:      0     185     468 
   Size:        185     283
 
ScaI AGT'ACT

Cuts at:      0     241     468 
   Size:        241     227
 
SmaI CCC'GGG

Cuts at:      0     346     444     468 
   Size:        346      98      24

  Fragments arranged by size: 

                 346      98      24
 
SmlI C'TyrA_G

Cuts at:      0      11     468 
   Size:         11     457
 
StuI AGG'CCT

Cuts at:      0      82     468 
   Size:         82     386
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     250     272     468 
   Size:        250      22     196

  Fragments arranged by size: 

                 250     196      22
 
TatI w'GTAC_w

Cuts at:      0     239     468 
   Size:        239     229
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     200     232     468 
   Size:        200      32     236

  Fragments arranged by size: 

                 236     200      32
 
XhoI C'TCGA_G

Cuts at:      0      11     468 
   Size:         11     457
 
XmaI C'CCGG_G

Cuts at:      0     344     442     468 
   Size:        344      98      26

  Fragments arranged by size: 

                 344      98      26
 

 Enzymes that do cut:

     AfeI     AvaI     BanI    BanII     BglI    BglII     BmrI     BpmI
     BsaI    BsaWI    BseRI    BsiEI  BsiHKAI Bsp1286I    BsrDI    BsrFI
    BstYI   Bsu36I     BtgI     EaeI     EarI  EcoICRI  Eco57MI    HaeII
   MspA1I    PvuII     SacI    SacII     ScaI     SmaI     SmlI     StuI
    TaqII     TatI     TstI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI  Tth111I     XbaI     XcmI     XmnI     ZraI