(Linear) (Six-base) MAPSORT of: KIEE3029.seq  Check: 1944  from: 1  to: 459



KIEE3029.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     459 
   Size:         86     373
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     308     459 
   Size:        308     151
 
ApoI r'AATT_y

Cuts at:      0     323     459 
   Size:        323     136
 
AvaI C'yCGr_G

Cuts at:      0     339     459 
   Size:        339     120
 
BanI G'GyrC_C

Cuts at:      0     159     459 
   Size:        159     300
 
Bme1580I G_kGCm'C

Cuts at:      0     162     459 
   Size:        162     297
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      12     266     459 
   Size:         12     254     193

  Fragments arranged by size: 

                 254     193      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     251     459 
   Size:        207      44     208

  Fragments arranged by size: 

                 208     207      44
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     459 
   Size:        148     311
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      60     459 
   Size:         60     399
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     459 
   Size:        142     317
 
Bsp1286I G_dGCh'C

Cuts at:      0     162     459 
   Size:        162     297
 
Bsu36I CC'TnA_GG

Cuts at:      0      72     179     459 
   Size:         72     107     280

  Fragments arranged by size: 

                 280     107      72
 
BtsI GCAGTG_nn'

Cuts at:      0     359     377     459 
   Size:        359      18      82

  Fragments arranged by size: 

                 359      82      18
 
EaeI y'GGCC_r

Cuts at:      0      35     459 
   Size:         35     424
 
EarI CTCTTCn'nnn_

Cuts at:      0     313     459 
   Size:        313     146
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     327     459 
   Size:        327     132
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     207     251     459 
   Size:         86     121      44     208

  Fragments arranged by size: 

                 208     121      86      44
 
EcoRI G'AATT_C

Cuts at:      0     323     459 
   Size:        323     136
 
MscI TGG'CCA

Cuts at:      0      37     459 
   Size:         37     422
 
MspA1I CmG'CkG

Cuts at:      0     135     459 
   Size:        135     324
 
NspI r_CATG'y

Cuts at:      0      57     459 
   Size:         57     402
 
PstI C_TGCA'G

Cuts at:      0     332     354     459 
   Size:        332      22     105

  Fragments arranged by size: 

                 332     105      22
 
PvuII CAG'CTG

Cuts at:      0     135     459 
   Size:        135     324
 
SfcI C'TryA_G

Cuts at:      0      58     328     350     459 
   Size:         58     270      22     109

  Fragments arranged by size: 

                 270     109      58      22
 
SmlI C'TyrA_G

Cuts at:      0     127     459 
   Size:        127     332
 
SphI G_CATG'C

Cuts at:      0      57     459 
   Size:         57     402
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     AvaI     BanI Bme1580I     BmrI     BpmI
    BpuEI    BseRI     BsgI Bsp1286I   Bsu36I     BtsI     EaeI     EarI
     EciI  Eco57MI    EcoRI     MscI   MspA1I     NspI     PstI    PvuII
     SfcI     SmlI     SphI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI