(Linear) (Six-base) MAPSORT of: KIEE3028.seq  Check: 3579  from: 1  to: 453



KIEE3028.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:03  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     142     453 
   Size:        142     311
 
AvaI C'yCGr_G

Cuts at:      0      25     453 
   Size:         25     428
 
BanI G'GyrC_C

Cuts at:      0     148     309     453 
   Size:        148     161     144

  Fragments arranged by size: 

                 161     148     144
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131     453 
   Size:        131     322
 
BfrBI ATG'CAT

Cuts at:      0     317     453 
   Size:        317     136
 
BglII A'GATC_T

Cuts at:      0      21     453 
   Size:         21     432
 
Bme1580I G_kGCm'C

Cuts at:      0     151     453 
   Size:        151     302
 
BmgBI CAC'GTC

Cuts at:      0     129     453 
   Size:        129     324
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      74     453 
   Size:         74     379
 
BsaBI GATnn'nnATC

Cuts at:      0     337     453 
   Size:        337     116
 
BsaHI Gr'CG_yC

Cuts at:      0     242     453 
   Size:        242     211
 
BseYI C'CCAG_C

Cuts at:      0     237     453 
   Size:        237     216
 
BsiHKAI G_wGCw'C

Cuts at:      0      17     453 
   Size:         17     436
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     151     453 
   Size:         17     134     302

  Fragments arranged by size: 

                 302     134      17
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     383     453 
   Size:        383      70
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237     453 
   Size:        237     216
 
BstYI r'GATC_y

Cuts at:      0      21     338     453 
   Size:         21     317     115

  Fragments arranged by size: 

                 317     115      21
 
BtsI GCAGTG_nn'

Cuts at:      0      51     453 
   Size:         51     402
 
EarI CTCTTCn'nnn_

Cuts at:      0       5     400     453 
   Size:          5     395      53

  Fragments arranged by size: 

                 395      53       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     409     453 
   Size:        409      44
 
EcoRV GAT'ATC

Cuts at:      0      48     453 
   Size:         48     405
 
MslI CAynn'nnrTG

Cuts at:      0     430     453 
   Size:        430      23
 
NsiI A_TGCA'T

Cuts at:      0     319     453 
   Size:        319     134
 
PasI CC'CwG_GG

Cuts at:      0     405     453 
   Size:        405      48
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     209     453 
   Size:        209     244
 
PstI C_TGCA'G

Cuts at:      0     421     453 
   Size:        421      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0       5     453 
   Size:          5     448
 
ScaI AGT'ACT

Cuts at:      0     208     453 
   Size:        208     245
 
SexAI A'CCwGG_T

Cuts at:      0      68     453 
   Size:         68     385
 
SfcI C'TryA_G

Cuts at:      0     153     417     453 
   Size:        153     264      36

  Fragments arranged by size: 

                 264     153      36
 
SmlI C'TyrA_G

Cuts at:      0      25     453 
   Size:         25     428
 
TatI w'GTAC_w

Cuts at:      0     206     453 
   Size:        206     247
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     356     388     453 
   Size:        356      32      65

  Fragments arranged by size: 

                 356      65      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     106     453 
   Size:        106     347
 
XhoI C'TCGA_G

Cuts at:      0      25     453 
   Size:         25     428
 

 Enzymes that do cut:

    AlwNI     AvaI     BanI     BbsI    BfrBI    BglII Bme1580I    BmgBI
     BsaI    BsaBI    BsaHI    BseYI  BsiHKAI Bsp1286I   BstAPI    BstXI
    BstYI     BtsI     EarI    EcoNI    EcoRV     MslI     NsiI     PasI
    PflMI     PstI     SapI     ScaI    SexAI     SfcI     SmlI     TatI
     TstI  Tth111I     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     XbaI     XcmI     XmaI     XmnI
     ZraI