(Linear) (Six-base) MAPSORT of: KIEE3027.seq  Check: 9752  from: 1  to: 453



KIEE3027.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

ApoI r'AATT_y

Cuts at:      0      32     453 
   Size:         32     421
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     200     415     453 
   Size:        200     215      38

  Fragments arranged by size: 

                 215     200      38
 
BfrBI ATG'CAT

Cuts at:      0     217     453 
   Size:        217     236
 
BsaWI w'CCGG_w

Cuts at:      0      93     453 
   Size:         93     360
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238     409     453 
   Size:        238     171      44

  Fragments arranged by size: 

                 238     171      44
 
BsmI GAATG_Cn'

Cuts at:      0     217     453 
   Size:        217     236
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     176     453 
   Size:        176     277
 
EarI CTCTTCn'nnn_

Cuts at:      0     257     284     321     335     453 
   Size:        257      27      37      14     118

  Fragments arranged by size: 

                 257     118      37      27      14
 
EcoRI G'AATT_C

Cuts at:      0      32     453 
   Size:         32     421
 
EcoRV GAT'ATC

Cuts at:      0     275     453 
   Size:        275     178
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     453 
   Size:         41     412
 
NsiI A_TGCA'T

Cuts at:      0     219     453 
   Size:        219     234
 
PshAI GACnn'nnGTC

Cuts at:      0     298     453 
   Size:        298     155
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      37     453 
   Size:         37     416
 
XbaI T'CTAG_A

Cuts at:      0     138     453 
   Size:        138     315
 

 Enzymes that do cut:

     ApoI     BbsI    BfrBI    BsaWI    BseRI     BsmI    BstXI     EarI
    EcoRI    EcoRV     MmeI     NsiI    PshAI    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI