(Linear) (Six-base) MAPSORT of: KIEE3024.seq  Check: 2048  from: 1  to: 423



KIEE3024.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:03  ..

AflIII A'CryG_T

Cuts at:      0     311     423 
   Size:        311     112
 
BanII G_rGCy'C

Cuts at:      0     223     423 
   Size:        223     200
 
BglII A'GATC_T

Cuts at:      0     241     423 
   Size:        241     182
 
Bme1580I G_kGCm'C

Cuts at:      0     283     423 
   Size:        283     140
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     207     217     423 
   Size:        115      92      10     206

  Fragments arranged by size: 

                 206     115      92      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     377     423 
   Size:        377      46
 
BseYI C'CCAG_C

Cuts at:      0     105     276     423 
   Size:        105     171     147

  Fragments arranged by size: 

                 171     147     105
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     184     196     423 
   Size:        140      44      12     227

  Fragments arranged by size: 

                 227     140      44      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     345     423 
   Size:        345      78
 
BsmI GAATG_Cn'

Cuts at:      0     420     423 
   Size:        420       3
 
Bsp1286I G_dGCh'C

Cuts at:      0     223     283     345     423 
   Size:        223      60      62      78

  Fragments arranged by size: 

                 223      78      62      60
 
BssHII G'CGCG_C

Cuts at:      0     139     423 
   Size:        139     284
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     195     375     423 
   Size:        195     180      48

  Fragments arranged by size: 

                 195     180      48
 
BstYI r'GATC_y

Cuts at:      0     241     423 
   Size:        241     182
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     373     423 
   Size:        373      50
 
EaeI y'GGCC_r

Cuts at:      0     116     423 
   Size:        116     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     207     217     423 
   Size:        115      92      10     206

  Fragments arranged by size: 

                 206     115      92      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      52     423 
   Size:         52     371
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     372     404     423 
   Size:        372      32      19

  Fragments arranged by size: 

                 372      32      19
 
MfeI C'AATT_G

Cuts at:      0     329     423 
   Size:        329      94
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     318     423 
   Size:        318     105
 
NspI r_CATG'y

Cuts at:      0     276     408     423 
   Size:        276     132      15

  Fragments arranged by size: 

                 276     132      15
 
ScaI AGT'ACT

Cuts at:      0     267     423 
   Size:        267     156
 
SphI G_CATG'C

Cuts at:      0     276     408     423 
   Size:        276     132      15

  Fragments arranged by size: 

                 276     132      15
 
TatI w'GTAC_w

Cuts at:      0     265     423 
   Size:        265     158
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     328     423 
   Size:        328      95
 

 Enzymes that do cut:

   AflIII    BanII    BglII Bme1580I     BpmI     BsaI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I   BssHII    BstXI    BstYI    BtgZI     EaeI
  Eco57MI    EcoNI    Hin4I     MfeI     MmeI     NspI     ScaI     SphI
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI