(Linear) (Six-base) MAPSORT of: KIEE3021.seq  Check: 1432  from: 1  to: 324



KIEE3021.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     149     324 
   Size:        149     175
 
BclI T'GATC_A

Cuts at:      0      86     324 
   Size:         86     238
 
BfrBI ATG'CAT

Cuts at:      0     246     324 
   Size:        246      78
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     199     324 
   Size:        199     125
 
BsaHI Gr'CG_yC

Cuts at:      0      77     324 
   Size:         77     247
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      14      44      94     124     324 
   Size:         14      30      50      30     200

  Fragments arranged by size: 

                 200      50      30      30      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18      22     324 
   Size:         18       4     302

  Fragments arranged by size: 

                 302      18       4
 
BseYI C'CCAG_C

Cuts at:      0      70     324 
   Size:         70     254
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     121     324 
   Size:        121     203
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     252     324 
   Size:        252      72
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      94     126     231     263     324 
   Size:         94      32     105      32      61

  Fragments arranged by size: 

                 105      94      61      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     178     324 
   Size:        178     146
 
MspA1I CmG'CkG

Cuts at:      0      51     200     324 
   Size:         51     149     124

  Fragments arranged by size: 

                 149     124      51
 
NsiI A_TGCA'T

Cuts at:      0     248     324 
   Size:        248      76
 
NspI r_CATG'y

Cuts at:      0     132     324 
   Size:        132     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     232     324 
   Size:        232      92
 
PvuII CAG'CTG

Cuts at:      0     200     324 
   Size:        200     124
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      14      46     324 
   Size:         14      32     278

  Fragments arranged by size: 

                 278      32      14
 

 Enzymes that do cut:

    AlwNI     BclI    BfrBI     BmrI    BsaHI    BsaXI    BseRI    BseYI
    BstXI     EciI    Hin4I     MslI   MspA1I     NsiI     NspI    PflMI
    PvuII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI