Restriction map for
pFN21AE3020
(Linear) (Six-base) MAPSORT of: KIEE3020.seq Check: 8716 from: 1 to: 375
KIEE3020.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AatII G_ACGT'C
Cuts at: 0 269 375
Size: 269 106
AvaI C'yCGr_G
Cuts at: 0 192 375
Size: 192 183
BanI G'GyrC_C
Cuts at: 0 54 205 375
Size: 54 151 170
Fragments arranged by size:
170 151 54
BbeI G_GCGC'C
Cuts at: 0 58 209 375
Size: 58 151 166
Fragments arranged by size:
166 151 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 330 375
Size: 330 45
BglI GCCn_nnn'nGGC
Cuts at: 0 34 375
Size: 34 341
Bme1580I G_kGCm'C
Cuts at: 0 95 375
Size: 95 280
BsaHI Gr'CG_yC
Cuts at: 0 55 206 266 375
Size: 55 151 60 109
Fragments arranged by size:
151 109 60 55
BseYI C'CCAG_C
Cuts at: 0 167 375
Size: 167 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 375
Size: 211 164
BsiEI CG_ry'CG
Cuts at: 0 26 309 375
Size: 26 283 66
Fragments arranged by size:
283 66 26
BsiWI C'GTAC_G
Cuts at: 0 152 375
Size: 152 223
Bsp1286I G_dGCh'C
Cuts at: 0 95 375
Size: 95 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 287 375
Size: 134 153 88
Fragments arranged by size:
153 134 88
BsrBI CCG'CTC
Cuts at: 0 294 375
Size: 294 81
BsrFI r'CCGG_y
Cuts at: 0 202 375
Size: 202 173
BssHII G'CGCG_C
Cuts at: 0 351 375
Size: 351 24
BtgI C'CryG_G
Cuts at: 0 119 309 375
Size: 119 190 66
Fragments arranged by size:
190 119 66
EaeI y'GGCC_r
Cuts at: 0 23 306 375
Size: 23 283 69
Fragments arranged by size:
283 69 23
EagI C'GGCC_G
Cuts at: 0 23 306 375
Size: 23 283 69
Fragments arranged by size:
283 69 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 375
Size: 199 176
EcoO109I rG'GnC_Cy
Cuts at: 0 146 314 375
Size: 146 168 61
Fragments arranged by size:
168 146 61
HaeII r_GCGC'y
Cuts at: 0 40 58 165 209 375
Size: 40 18 107 44 166
Fragments arranged by size:
166 107 44 40 18
KasI G'GCGC_C
Cuts at: 0 54 205 375
Size: 54 151 170
Fragments arranged by size:
170 151 54
MspA1I CmG'CkG
Cuts at: 0 85 121 311 375
Size: 85 36 190 64
Fragments arranged by size:
190 85 64 36
NaeI GCC'GGC
Cuts at: 0 204 375
Size: 204 171
NarI GG'CG_CC
Cuts at: 0 55 206 375
Size: 55 151 169
Fragments arranged by size:
169 151 55
NgoMIV G'CCGG_C
Cuts at: 0 202 375
Size: 202 173
NotI GC'GGCC_GC
Cuts at: 0 23 375
Size: 23 352
PfoI T'CCnGG_A
Cuts at: 0 260 375
Size: 260 115
PpuMI rG'GwC_Cy
Cuts at: 0 146 314 375
Size: 146 168 61
Fragments arranged by size:
168 146 61
PstI C_TGCA'G
Cuts at: 0 284 375
Size: 284 91
RsrII CG'GwC_CG
Cuts at: 0 8 375
Size: 8 367
SacII CC_GC'GG
Cuts at: 0 122 312 375
Size: 122 190 63
Fragments arranged by size:
190 122 63
SfcI C'TryA_G
Cuts at: 0 174 280 375
Size: 174 106 95
Fragments arranged by size:
174 106 95
SfoI GGC'GCC
Cuts at: 0 56 207 375
Size: 56 151 168
Fragments arranged by size:
168 151 56
SmaI CCC'GGG
Cuts at: 0 194 375
Size: 194 181
XmaI C'CCGG_G
Cuts at: 0 192 375
Size: 192 183
XmnI GAAnn'nnTTC
Cuts at: 0 337 375
Size: 337 38
ZraI GAC'GTC
Cuts at: 0 267 375
Size: 267 108
Enzymes that do cut:
AatII AvaI BanI BbeI BbsI BglI Bme1580I BsaHI
BseYI BsgI BsiEI BsiWI Bsp1286I BspMI BsrBI BsrFI
BssHII BtgI EaeI EagI EciI EcoO109I HaeII KasI
MspA1I NaeI NarI NgoMIV NotI PfoI PpuMI PstI
RsrII SacII SfcI SfoI SmaI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BsiHKAI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NcoI NdeI
NheI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI