Restriction map for
pFN21AE3019
(Linear) (Six-base) MAPSORT of: KIEE3019.seq Check: 4796 from: 1 to: 372
KIEE3019.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 290 372
Size: 290 82
AfeI AGC'GCT
Cuts at: 0 23 181 372
Size: 23 158 191
Fragments arranged by size:
191 158 23
ApaI G_GGCC'C
Cuts at: 0 153 203 372
Size: 153 50 169
Fragments arranged by size:
169 153 50
AvaI C'yCGr_G
Cuts at: 0 82 105 124 217 372
Size: 82 23 19 93 155
Fragments arranged by size:
155 93 82 23 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 40 73 372
Size: 40 33 299
Fragments arranged by size:
299 40 33
BanII G_rGCy'C
Cuts at: 0 82 124 153 203 372
Size: 82 42 29 50 169
Fragments arranged by size:
169 82 50 42 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 336 370 372
Size: 336 34 2
Fragments arranged by size:
336 34 2
Bme1580I G_kGCm'C
Cuts at: 0 61 132 153 203 372
Size: 61 71 21 50 169
Fragments arranged by size:
169 71 61 50 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 319 372
Size: 138 181 53
Fragments arranged by size:
181 138 53
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 151 372
Size: 90 61 221
Fragments arranged by size:
221 90 61
BsiEI CG_ry'CG
Cuts at: 0 92 207 238 372
Size: 92 115 31 134
Fragments arranged by size:
134 115 92 31
Bsp1286I G_dGCh'C
Cuts at: 0 61 82 124 132 153 203 372
Size: 61 21 42 8 21 50 169
Fragments arranged by size:
169 61 50 42 21 21 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 372
Size: 290 82
BsrBI CCG'CTC
Cuts at: 0 234 372
Size: 234 138
BssHII G'CGCG_C
Cuts at: 0 155 330 372
Size: 155 175 42
Fragments arranged by size:
175 155 42
BtgI C'CryG_G
Cuts at: 0 238 372
Size: 238 134
EaeI y'GGCC_r
Cuts at: 0 29 89 204 235 372
Size: 29 60 115 31 137
Fragments arranged by size:
137 115 60 31 29
EagI C'GGCC_G
Cuts at: 0 89 204 235 372
Size: 89 115 31 137
Fragments arranged by size:
137 115 89 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 190 372
Size: 190 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 319 372
Size: 138 181 53
Fragments arranged by size:
181 138 53
EcoO109I rG'GnC_Cy
Cuts at: 0 102 149 199 372
Size: 102 47 50 173
Fragments arranged by size:
173 102 50 47
HaeII r_GCGC'y
Cuts at: 0 25 183 372
Size: 25 158 189
Fragments arranged by size:
189 158 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 200 232 372
Size: 200 32 140
Fragments arranged by size:
200 140 32
MspA1I CmG'CkG
Cuts at: 0 54 240 324 372
Size: 54 186 84 48
Fragments arranged by size:
186 84 54 48
NotI GC'GGCC_GC
Cuts at: 0 235 372
Size: 235 137
PasI CC'CwG_GG
Cuts at: 0 61 372
Size: 61 311
PfoI T'CCnGG_A
Cuts at: 0 98 372
Size: 98 274
PpuMI rG'GwC_Cy
Cuts at: 0 102 372
Size: 102 270
PshAI GACnn'nnGTC
Cuts at: 0 107 372
Size: 107 265
PspOMI G'GGCC_C
Cuts at: 0 149 199 372
Size: 149 50 173
Fragments arranged by size:
173 149 50
PvuII CAG'CTG
Cuts at: 0 54 372
Size: 54 318
SacII CC_GC'GG
Cuts at: 0 241 372
Size: 241 131
SmaI CCC'GGG
Cuts at: 0 126 372
Size: 126 246
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 372
Size: 126 32 214
Fragments arranged by size:
214 126 32
XmaI C'CCGG_G
Cuts at: 0 124 372
Size: 124 248
Enzymes that do cut:
AarI AfeI ApaI AvaI BaeI BanII BcgI Bme1580I
BpmI BseRI BsiEI Bsp1286I BspMI BsrBI BssHII BtgI
EaeI EagI EciI Eco57MI EcoO109I HaeII Hin4I MspA1I
NotI PasI PfoI PpuMI PshAI PspOMI PvuII SacII
SmaI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BamHI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI
ZraI