(Linear) (Six-base) MAPSORT of: KIEE3018.seq  Check: 5661  from: 1  to: 348



KIEE3018.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:03  ..

Acc65I G'GTAC_C

Cuts at:      0      70     348 
   Size:         70     278
 
AfeI AGC'GCT

Cuts at:      0     167     348 
   Size:        167     181
 
AflIII A'CryG_T

Cuts at:      0     201     348 
   Size:        201     147
 
AvaI C'yCGr_G

Cuts at:      0     104     308     348 
   Size:        104     204      40

  Fragments arranged by size: 

                 204     104      40
 
BanI G'GyrC_C

Cuts at:      0      37      70     348 
   Size:         37      33     278

  Fragments arranged by size: 

                 278      37      33
 
BanII G_rGCy'C

Cuts at:      0      10      98     111     348 
   Size:         10      88      13     237

  Fragments arranged by size: 

                 237      88      13      10
 
BbeI G_GCGC'C

Cuts at:      0      41     348 
   Size:         41     307
 
Bme1580I G_kGCm'C

Cuts at:      0      81     348 
   Size:         81     267
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     348 
   Size:         82     266
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     348 
   Size:        175     173
 
BsaHI Gr'CG_yC

Cuts at:      0      38      89     248     348 
   Size:         38      51     159     100

  Fragments arranged by size: 

                 159     100      51      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     348 
   Size:        284      64
 
BsiHKAI G_wGCw'C

Cuts at:      0      10      98     348 
   Size:         10      88     250

  Fragments arranged by size: 

                 250      88      10
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      81      98     111     348 
   Size:         10      71      17      13     237

  Fragments arranged by size: 

                 237      71      17      13      10
 
BsrFI r'CCGG_y

Cuts at:      0     250     348 
   Size:        250      98
 
BssSI C'ACGA_G

Cuts at:      0     145     348 
   Size:        145     203
 
BtgI C'CryG_G

Cuts at:      0     128     348 
   Size:        128     220
 
BtsI GCAGTG_nn'

Cuts at:      0     265     272     348 
   Size:        265       7      76

  Fragments arranged by size: 

                 265      76       7
 
EaeI y'GGCC_r

Cuts at:      0     228     348 
   Size:        228     120
 
EcoICRI GAG'CTC

Cuts at:      0       8      96     348 
   Size:          8      88     252

  Fragments arranged by size: 

                 252      88       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     348 
   Size:         82     266
 
HaeII r_GCGC'y

Cuts at:      0      41     169     348 
   Size:         41     128     179

  Fragments arranged by size: 

                 179     128      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      45      77     106     124     138     156     348 
   Size:         45      32      29      18      14      18     192

  Fragments arranged by size: 

                 192      45      32      29      18      18      14
 
KasI G'GCGC_C

Cuts at:      0      37     348 
   Size:         37     311
 
KpnI G_GTAC'C

Cuts at:      0      74     348 
   Size:         74     274
 
MscI TGG'CCA

Cuts at:      0     230     348 
   Size:        230     118
 
NarI GG'CG_CC

Cuts at:      0      38     348 
   Size:         38     310
 
SacI G_AGCT'C

Cuts at:      0      10      98     348 
   Size:         10      88     250

  Fragments arranged by size: 

                 250      88      10
 
SfoI GGC'GCC

Cuts at:      0      39     348 
   Size:         39     309
 
SgrAI Cr'CCGG_yG

Cuts at:      0     250     348 
   Size:        250      98
 
SmlI C'TyrA_G

Cuts at:      0     190     348 
   Size:        190     158
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     282     314     348 
   Size:        282      32      34

  Fragments arranged by size: 

                 282      34      32
 

 Enzymes that do cut:

   Acc65I     AfeI   AflIII     AvaI     BanI    BanII     BbeI Bme1580I
     BpmI    BpuEI    BsaHI     BsgI  BsiHKAI Bsp1286I    BsrFI    BssSI
     BtgI     BtsI     EaeI  EcoICRI  Eco57MI    HaeII    Hin4I     KasI
     KpnI     MscI     NarI     SacI     SfoI    SgrAI     SmlI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI