(Linear) (Six-base) MAPSORT of: KIEE3017.seq  Check: 304  from: 1  to: 348



KIEE3017.orf

 With 182 enzymes: SgfI * 

                        March 5, 2012 16:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     348 
   Size:        230     118
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     281     348 
   Size:        281      67
 
AlwNI CAG_nnn'CTG

Cuts at:      0     102     141     348 
   Size:        102      39     207

  Fragments arranged by size: 

                 207     102      39
 
ApoI r'AATT_y

Cuts at:      0     318     348 
   Size:        318      30
 
AvaI C'yCGr_G

Cuts at:      0     293     348 
   Size:        293      55
 
BanI G'GyrC_C

Cuts at:      0     288     348 
   Size:        288      60
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     337     348 
   Size:        337      11
 
BglII A'GATC_T

Cuts at:      0     213     262     348 
   Size:        213      49      86

  Fragments arranged by size: 

                 213      86      49
 
BlpI GC'TnA_GC

Cuts at:      0     230     348 
   Size:        230     118
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1     348 
   Size:          1     347
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     348 
   Size:        271      77
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      57      87     348 
   Size:         57      30     261

  Fragments arranged by size: 

                 261      57      30
 
BsrFI r'CCGG_y

Cuts at:      0      67     348 
   Size:         67     281
 
BssHII G'CGCG_C

Cuts at:      0      23     348 
   Size:         23     325
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48     348 
   Size:         48     300
 
BstYI r'GATC_y

Cuts at:      0     213     262     348 
   Size:        213      49      86

  Fragments arranged by size: 

                 213      86      49
 
BtgI C'CryG_G

Cuts at:      0     125     348 
   Size:        125     223
 
EaeI y'GGCC_r

Cuts at:      0     143     348 
   Size:        143     205
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1     230     348 
   Size:          1     229     118

  Fragments arranged by size: 

                 229     118       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     171     339     348 
   Size:        171     168       9

  Fragments arranged by size: 

                 171     168       9
 
HaeII r_GCGC'y

Cuts at:      0      74     348 
   Size:         74     274
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     154     186     348 
   Size:        154      32     162

  Fragments arranged by size: 

                 162     154      32
 
MscI TGG'CCA

Cuts at:      0     145     348 
   Size:        145     203
 
MspA1I CmG'CkG

Cuts at:      0     127     348 
   Size:        127     221
 
PasI CC'CwG_GG

Cuts at:      0      54     348 
   Size:         54     294
 
PstI C_TGCA'G

Cuts at:      0     107     260     348 
   Size:        107     153      88

  Fragments arranged by size: 

                 153     107      88
 
SacII CC_GC'GG

Cuts at:      0     128     348 
   Size:        128     220
 
ScaI AGT'ACT

Cuts at:      0     151     348 
   Size:        151     197
 
SfcI C'TryA_G

Cuts at:      0     103     256     348 
   Size:        103     153      92

  Fragments arranged by size: 

                 153     103      92
 
SgrAI Cr'CCGG_yG

Cuts at:      0      67     348 
   Size:         67     281
 
SmlI C'TyrA_G

Cuts at:      0     250     348 
   Size:        250      98
 
TatI w'GTAC_w

Cuts at:      0     149     348 
   Size:        149     199
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      57      89     348 
   Size:         57      32     259

  Fragments arranged by size: 

                 259      57      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      92     348 
   Size:         92     256
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     153     348 
   Size:        153     195
 
XmnI GAAnn'nnTTC

Cuts at:      0     322     334     348 
   Size:        322      12      14

  Fragments arranged by size: 

                 322      14      12
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApoI     AvaI     BanI     BbsI    BglII
     BlpI     BpmI    BpuEI    BsaXI    BsrFI   BssHII    BstXI    BstYI
     BtgI     EaeI  Eco57MI     FalI    HaeII    Hin4I     MscI   MspA1I
     PasI     PstI    SacII     ScaI     SfcI    SgrAI     SmlI     TatI
     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     XbaI     XhoI     XmaI     ZraI