(Linear) (Six-base) MAPSORT of: KIEE3016.seq  Check: 2561  from: 1  to: 342



KIEE3016.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:02  ..

AccI GT'mk_AC

Cuts at:      0     188     342 
   Size:        188     154
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      62     342 
   Size:         62     280
 
BanII G_rGCy'C

Cuts at:      0     203     342 
   Size:        203     139
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      15     342 
   Size:         15     327
 
BclI T'GATC_A

Cuts at:      0     227     342 
   Size:        227     115
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55     342 
   Size:         55     287
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     255     342 
   Size:         88     167      87

  Fragments arranged by size: 

                 167      88      87
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     233     342 
   Size:        233     109
 
BseYI C'CCAG_C

Cuts at:      0     329     342 
   Size:        329      13
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     342 
   Size:        203     139
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     279     342 
   Size:        279      63
 
EaeI y'GGCC_r

Cuts at:      0     293     342 
   Size:        293      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     255     342 
   Size:         88     167      87

  Fragments arranged by size: 

                 167      88      87
 
EcoO109I rG'GnC_Cy

Cuts at:      0     181     342 
   Size:        181     161
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     228     260     342 
   Size:        228      32      82

  Fragments arranged by size: 

                 228      82      32
 
MspA1I CmG'CkG

Cuts at:      0      46     279     342 
   Size:         46     233      63

  Fragments arranged by size: 

                 233      63      46
 
NspI r_CATG'y

Cuts at:      0      43     337     342 
   Size:         43     294       5

  Fragments arranged by size: 

                 294      43       5
 
PpuMI rG'GwC_Cy

Cuts at:      0     181     342 
   Size:        181     161
 
PvuII CAG'CTG

Cuts at:      0      46     279     342 
   Size:         46     233      63

  Fragments arranged by size: 

                 233      63      46
 
SfcI C'TryA_G

Cuts at:      0     185     342 
   Size:        185     157
 
SphI G_CATG'C

Cuts at:      0      43     337     342 
   Size:         43     294       5

  Fragments arranged by size: 

                 294      43       5
 

 Enzymes that do cut:

     AccI     AlfI    BanII    BciVI     BclI     BglI     BpmI    BseRI
    BseYI Bsp1286I    BstXI     EaeI  Eco57MI EcoO109I    Hin4I   MspA1I
     NspI    PpuMI    PvuII     SfcI     SphI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI