(Linear) (Six-base) MAPSORT of: KIEE3010.seq  Check: 2675  from: 1  to: 291



KIEE3010.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AccI GT'mk_AC

Cuts at:      0      77     291 
   Size:         77     214
 
AclI AA'CG_TT

Cuts at:      0     229     291 
   Size:        229      62
 
ApaI G_GGCC'C

Cuts at:      0     280     291 
   Size:        280      11
 
AvaI C'yCGr_G

Cuts at:      0      97     291 
   Size:         97     194
 
AvrII C'CTAG_G

Cuts at:      0      63     291 
   Size:         63     228
 
BanII G_rGCy'C

Cuts at:      0     280     291 
   Size:        280      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     119     291 
   Size:        119     172
 
Bme1580I G_kGCm'C

Cuts at:      0     280     291 
   Size:        280      11
 
BsaHI Gr'CG_yC

Cuts at:      0      80     116     291 
   Size:         80      36     175

  Fragments arranged by size: 

                 175      80      36
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     133     163     291 
   Size:        133      30     128

  Fragments arranged by size: 

                 133     128      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     142     291 
   Size:        142     149
 
BsiEI CG_ry'CG

Cuts at:      0     271     291 
   Size:        271      20
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     194     291 
   Size:        194      97
 
Bsp1286I G_dGCh'C

Cuts at:      0     280     291 
   Size:        280      11
 
BstEII G'GTnAC_C

Cuts at:      0     174     291 
   Size:        174     117
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     287     291 
   Size:        287       4
 
BtgI C'CryG_G

Cuts at:      0      19     291 
   Size:         19     272
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     256     291 
   Size:        256      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     276     291 
   Size:        276      15
 
FspI TGC'GCA

Cuts at:      0      13     291 
   Size:         13     278
 
HaeII r_GCGC'y

Cuts at:      0      73     291 
   Size:         73     218
 
HincII GTy'rAC

Cuts at:      0      78     291 
   Size:         78     213
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     291 
   Size:        251      40
 
MspA1I CmG'CkG

Cuts at:      0     186     291 
   Size:        186     105
 
PspOMI G'GGCC_C

Cuts at:      0     276     291 
   Size:        276      15
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     127     159     291 
   Size:        127      32     132

  Fragments arranged by size: 

                 132     127      32
 
SalI G'TCGA_C

Cuts at:      0      76     291 
   Size:         76     215
 
SexAI A'CCwGG_T

Cuts at:      0     170     291 
   Size:        170     121
 
StyI C'CwwG_G

Cuts at:      0      63     291 
   Size:         63     228
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     291 
   Size:         42     249
 

 Enzymes that do cut:

     AccI     AclI     ApaI     AvaI    AvrII    BanII     BbsI Bme1580I
    BsaHI    BsaXI    BseRI    BsiEI    BsmBI Bsp1286I   BstEII    BstXI
     BtgI     DrdI EcoO109I     FspI    HaeII   HincII     MmeI   MspA1I
   PspOMI     PsrI     SalI    SexAI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI