(Linear) (Six-base) MAPSORT of: KIEE3009.seq  Check: 2101  from: 1  to: 276



KIEE3009.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     276 
   Size:        141     135
 
ApoI r'AATT_y

Cuts at:      0      76     196     276 
   Size:         76     120      80

  Fragments arranged by size: 

                 120      80      76
 
AvaI C'yCGr_G

Cuts at:      0      37     276 
   Size:         37     239
 
BamHI G'GATC_C

Cuts at:      0     219     276 
   Size:        219      57
 
BlpI GC'TnA_GC

Cuts at:      0      46     276 
   Size:         46     230
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     276 
   Size:         82     194
 
BstYI r'GATC_y

Cuts at:      0     219     276 
   Size:        219      57
 
Bsu36I CC'TnA_GG

Cuts at:      0     115     276 
   Size:        115     161
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     141     276 
   Size:         82      59     135

  Fragments arranged by size: 

                 135      82      59
 
EcoO109I rG'GnC_Cy

Cuts at:      0     119     276 
   Size:        119     157
 
HaeII r_GCGC'y

Cuts at:      0      46     276 
   Size:         46     230
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      75     107     276 
   Size:         75      32     169

  Fragments arranged by size: 

                 169      75      32
 
MspA1I CmG'CkG

Cuts at:      0      27      60      69     276 
   Size:         27      33       9     207

  Fragments arranged by size: 

                 207      33      27       9
 
NspI r_CATG'y

Cuts at:      0     249     276 
   Size:        249      27
 
PpuMI rG'GwC_Cy

Cuts at:      0     119     276 
   Size:        119     157
 
PstI C_TGCA'G

Cuts at:      0      26     207     276 
   Size:         26     181      69

  Fragments arranged by size: 

                 181      69      26
 
PvuII CAG'CTG

Cuts at:      0      60      69     276 
   Size:         60       9     207

  Fragments arranged by size: 

                 207      60       9
 
SfcI C'TryA_G

Cuts at:      0      22     203     276 
   Size:         22     181      73

  Fragments arranged by size: 

                 181      73      22
 
SmaI CCC'GGG

Cuts at:      0      39     276 
   Size:         39     237
 
SspI AAT'ATT

Cuts at:      0     167     276 
   Size:        167     109
 
XmaI C'CCGG_G

Cuts at:      0      37     276 
   Size:         37     239
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BamHI     BlpI     BpmI    BstYI   Bsu36I
  Eco57MI EcoO109I    HaeII    Hin4I   MspA1I     NspI    PpuMI     PstI
    PvuII     SfcI     SmaI     SspI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI