(Linear) (Six-base) MAPSORT of: KIEE3007.seq  Check: 994  from: 1  to: 261



KIEE3007.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:56  ..

AatII G_ACGT'C

Cuts at:      0     188     261 
   Size:        188      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     114     147     261 
   Size:        114      33     114

  Fragments arranged by size: 

                 114     114      33
 
BbvCI CC'TCA_GC

Cuts at:      0      10     261 
   Size:         10     251
 
Bpu10I CC'TnA_GC

Cuts at:      0      10     261 
   Size:         10     251
 
BsaHI Gr'CG_yC

Cuts at:      0     185     261 
   Size:        185      76
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     180     261 
   Size:        180      81
 
BseYI C'CCAG_C

Cuts at:      0      39     261 
   Size:         39     222
 
BsiEI CG_ry'CG

Cuts at:      0     162     261 
   Size:        162      99
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     226     261 
   Size:        226      35
 
BstYI r'GATC_y

Cuts at:      0      92     261 
   Size:         92     169
 
BtgI C'CryG_G

Cuts at:      0      86     261 
   Size:         86     175
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     261 
   Size:         14     247
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     261 
   Size:        142     119
 
NcoI C'CATG_G

Cuts at:      0      86     261 
   Size:         86     175
 
PasI CC'CwG_GG

Cuts at:      0     177     261 
   Size:        177      84
 
StuI AGG'CCT

Cuts at:      0     144     261 
   Size:        144     117
 
StyI C'CwwG_G

Cuts at:      0      86     261 
   Size:         86     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     148     261 
   Size:        148     113
 
ZraI GAC'GTC

Cuts at:      0     186     261 
   Size:        186      75
 

 Enzymes that do cut:

    AatII     BaeI    BbvCI   Bpu10I    BsaHI    BseRI    BseYI    BsiEI
    BspMI    BstYI     BtgI EcoO109I     MmeI     NcoI     PasI     StuI
     StyI    TaqII     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI