(Linear) (Six-base) MAPSORT of: KIEE3005.seq  Check: 4300  from: 1  to: 249



KIEE3005.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

BanII G_rGCy'C

Cuts at:      0     230     249 
   Size:        230      19
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     107     128     249 
   Size:        107      21     121

  Fragments arranged by size: 

                 121     107      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     249 
   Size:         11     238
 
BsiHKAI G_wGCw'C

Cuts at:      0     230     249 
   Size:        230      19
 
BsmI GAATG_Cn'

Cuts at:      0     202     249 
   Size:        202      47
 
Bsp1286I G_dGCh'C

Cuts at:      0     230     249 
   Size:        230      19
 
BtgI C'CryG_G

Cuts at:      0     193     249 
   Size:        193      56
 
EcoICRI GAG'CTC

Cuts at:      0     228     249 
   Size:        228      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     249 
   Size:         11     238
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43      64     249 
   Size:         43      21     185

  Fragments arranged by size: 

                 185      43      21
 
EcoRV GAT'ATC

Cuts at:      0     177     249 
   Size:        177      72
 
NcoI C'CATG_G

Cuts at:      0     193     249 
   Size:        193      56
 
PfoI T'CCnGG_A

Cuts at:      0     150     249 
   Size:        150      99
 
PpuMI rG'GwC_Cy

Cuts at:      0      43     249 
   Size:         43     206
 
SacI G_AGCT'C

Cuts at:      0     230     249 
   Size:        230      19
 
StyI C'CwwG_G

Cuts at:      0     193     249 
   Size:        193      56
 

 Enzymes that do cut:

    BanII     BplI     BpmI  BsiHKAI     BsmI Bsp1286I     BtgI  EcoICRI
  Eco57MI EcoO109I    EcoRV     NcoI     PfoI    PpuMI     SacI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI