(Linear) (Six-base) MAPSORT of: KIEE3003.seq  Check: 4889  from: 1  to: 210



KIEE3003.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     210 
   Size:         73     137
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     140     173     210 
   Size:        140      33      37

  Fragments arranged by size: 

                 140      37      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     109     143     210 
   Size:        109      34      67

  Fragments arranged by size: 

                 109      67      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     210 
   Size:         79     131
 
BsaHI Gr'CG_yC

Cuts at:      0     151     210 
   Size:        151      59
 
BsiEI CG_ry'CG

Cuts at:      0      75     210 
   Size:         75     135
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     115     210 
   Size:        115      95
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12     210 
   Size:         12     198
 
BtgI C'CryG_G

Cuts at:      0     164     210 
   Size:        164      46
 
EaeI y'GGCC_r

Cuts at:      0      14     210 
   Size:         14     196
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73      79     210 
   Size:         73       6     131

  Fragments arranged by size: 

                 131      73       6
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210 
   Size:        210
 
HaeII r_GCGC'y

Cuts at:      0     192     210 
   Size:        192      18
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     210 
   Size:         29     181
 
MscI TGG'CCA

Cuts at:      0      16     210 
   Size:         16     194
 
MslI CAynn'nnrTG

Cuts at:      0      82     210 
   Size:         82     128
 
MspA1I CmG'CkG

Cuts at:      0      51     100     144     166     210 
   Size:         51      49      44      22      44

  Fragments arranged by size: 

                  51      49      44      44      22
 
NspI r_CATG'y

Cuts at:      0     130     210 
   Size:        130      80
 
PvuII CAG'CTG

Cuts at:      0      51     210 
   Size:         51     159
 
SacII CC_GC'GG

Cuts at:      0     167     210 
   Size:        167      43
 
SphI G_CATG'C

Cuts at:      0     130     210 
   Size:        130      80
 
XmnI GAAnn'nnTTC

Cuts at:      0     175     210 
   Size:        175      35
 

 Enzymes that do cut:

     AcuI     BaeI     BcgI     BpmI    BsaHI    BsiEI    BsmBI    BstXI
     BtgI     EaeI  Eco57MI     FalI    HaeII     MmeI     MscI     MslI
   MspA1I     NspI    PvuII    SacII     SphI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI