Restriction map for
pFN21AE2798
(Linear) (Six-base) MAPSORT of: KIEE2798.seq Check: 9627 from: 1 to: 1770
KIEE2798.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1176 1770
Size: 1176 594
AccI GT'mk_AC
Cuts at: 0 467 1770
Size: 467 1303
AlwNI CAG_nnn'CTG
Cuts at: 0 1632 1770
Size: 1632 138
ApaI G_GGCC'C
Cuts at: 0 54 630 738 900 954 1770
Size: 54 576 108 162 54 816
Fragments arranged by size:
816 576 162 108 54 54
BamHI G'GATC_C
Cuts at: 0 680 1770
Size: 680 1090
BanI G'GyrC_C
Cuts at: 0 1543 1770
Size: 1543 227
BanII G_rGCy'C
Cuts at: 0 54 269 630 738 900 954 1770
Size: 54 215 361 108 162 54 816
Fragments arranged by size:
816 361 215 162 108 54 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 1208 1770
Size: 1208 562
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 457 491 1770
Size: 457 34 1279
Fragments arranged by size:
1279 457 34
BfrBI ATG'CAT
Cuts at: 0 3 1770
Size: 3 1767
BglI GCCn_nnn'nGGC
Cuts at: 0 236 430 1702 1770
Size: 236 194 1272 68
Fragments arranged by size:
1272 236 194 68
BlpI GC'TnA_GC
Cuts at: 0 118 1770
Size: 118 1652
Bme1580I G_kGCm'C
Cuts at: 0 54 630 738 900 954 1548 1770
Size: 54 576 108 162 54 594 222
Fragments arranged by size:
594 576 222 162 108 54 54
BmgBI CAC'GTC
Cuts at: 0 13 1770
Size: 13 1757
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 1454 1770
Size: 55 1399 316
Fragments arranged by size:
1399 316 55
BsaI GGTCTCn'nnnn_
Cuts at: 0 643 805 1021 1770
Size: 643 162 216 749
Fragments arranged by size:
749 643 216 162
BsaHI Gr'CG_yC
Cuts at: 0 257 1770
Size: 257 1513
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1761 1770
Size: 1761 9
BseYI C'CCAG_C
Cuts at: 0 499 553 607 985 1297 1459 1770
Size: 499 54 54 378 312 162 311
Fragments arranged by size:
499 378 312 311 162 54 54
BsmI GAATG_Cn'
Cuts at: 0 1590 1770
Size: 1590 180
Bsp1286I G_dGCh'C
Cuts at: 0 54 269 630 738 900 954 1548 1770
Size: 54 215 361 108 162 54 594 222
Fragments arranged by size:
594 361 222 215 162 108 54 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1176 1770
Size: 1176 594
BsrDI GCAATG_nn'
Cuts at: 0 129 1770
Size: 129 1641
BsrFI r'CCGG_y
Cuts at: 0 235 694 1770
Size: 235 459 1076
Fragments arranged by size:
1076 459 235
BssSI C'ACGA_G
Cuts at: 0 1345 1770
Size: 1345 425
BstXI CCAn_nnnn'nTGG
Cuts at: 0 313 856 1459 1558 1770
Size: 313 543 603 99 212
Fragments arranged by size:
603 543 313 212 99
BstYI r'GATC_y
Cuts at: 0 680 1654 1770
Size: 680 974 116
Fragments arranged by size:
974 680 116
BtgI C'CryG_G
Cuts at: 0 273 306 348 633 795 1011 1107 1119
Size: 273 33 42 285 162 216 96 12
Cuts at: 1119 1770
Size: 651
Fragments arranged by size:
651 285 273 216 162 96 42 33
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 1770
Size: 89 1681
DrdI GACnn_nn'nnGTC
Cuts at: 0 256 1210 1426 1770
Size: 256 954 216 344
Fragments arranged by size:
954 344 256 216
EaeI y'GGCC_r
Cuts at: 0 68 345 456 510 564 618 672 726
Size: 68 277 111 54 54 54 54 54
Cuts at: 726 780 834 888 942 996 1050 1104 1158
Size: 54 54 54 54 54 54 54 54
Cuts at: 1158 1266 1374 1482 1536 1560 1770
Size: 108 108 108 54 24 210
Fragments arranged by size:
277 210 111 108 108 108 68 54
54 54 54 54 54 54 54 54
54 54 54 54 54 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1399 1770
Size: 1399 371
EcoO109I rG'GnC_Cy
Cuts at: 0 50 51 147 626 734 896 950 1703
Size: 50 1 96 479 108 162 54 753
Cuts at: 1703 1770
Size: 67
Fragments arranged by size:
753 479 162 108 96 67 54 50
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1673 1770
Size: 1673 97
HincII GTy'rAC
Cuts at: 0 357 468 1494 1602 1713 1770
Size: 357 111 1026 108 111 57
Fragments arranged by size:
1026 357 111 111 108 57
HindIII A'AGCT_T
Cuts at: 0 340 1531 1770
Size: 340 1191 239
Fragments arranged by size:
1191 340 239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1356 1770
Size: 227 1129 414
Fragments arranged by size:
1129 414 227
MscI TGG'CCA
Cuts at: 0 347 458 512 566 620 674 728 782
Size: 347 111 54 54 54 54 54 54
Cuts at: 782 836 890 944 998 1052 1160 1268 1376
Size: 54 54 54 54 54 108 108 108
Cuts at: 1376 1484 1538 1562 1770
Size: 108 54 24 208
Fragments arranged by size:
347 208 111 108 108 108 108 54
54 54 54 54 54 54 54 54
54 54 54 24
MspA1I CmG'CkG
Cuts at: 0 1629 1770
Size: 1629 141
NcoI C'CATG_G
Cuts at: 0 306 348 633 795 1011 1107 1119 1770
Size: 306 42 285 162 216 96 12 651
Fragments arranged by size:
651 306 285 216 162 96 42 12
NsiI A_TGCA'T
Cuts at: 0 5 1770
Size: 5 1765
NspI r_CATG'y
Cuts at: 0 181 1770
Size: 181 1589
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 1770
Size: 279 1491
PspOMI G'GGCC_C
Cuts at: 0 50 626 734 896 950 1770
Size: 50 576 108 162 54 820
Fragments arranged by size:
820 576 162 108 54 50
PstI C_TGCA'G
Cuts at: 0 480 534 588 1583 1770
Size: 480 54 54 995 187
Fragments arranged by size:
995 480 187 54 54
PvuII CAG'CTG
Cuts at: 0 1629 1770
Size: 1629 141
SalI G'TCGA_C
Cuts at: 0 466 1770
Size: 466 1304
SfcI C'TryA_G
Cuts at: 0 476 530 584 1579 1770
Size: 476 54 54 995 191
Fragments arranged by size:
995 476 191 54 54
SphI G_CATG'C
Cuts at: 0 181 1770
Size: 181 1589
StuI AGG'CCT
Cuts at: 0 1135 1220 1770
Size: 1135 85 550
Fragments arranged by size:
1135 550 85
StyI C'CwwG_G
Cuts at: 0 306 348 633 795 1011 1107 1119 1215
Size: 306 42 285 162 216 96 12 96
Cuts at: 1215 1431 1485 1539 1770
Size: 216 54 54 231
Fragments arranged by size:
306 285 231 216 216 162 96 96
54 54 42 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1698 1770
Size: 1698 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 176 208 1508 1540 1770
Size: 176 32 1300 32 230
Fragments arranged by size:
1300 230 176 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 853 1555 1633 1770
Size: 853 702 78 137
Fragments arranged by size:
853 702 137 78
XmnI GAAnn'nnTTC
Cuts at: 0 202 1770
Size: 202 1568
Enzymes that do cut:
AarI AccI AlwNI ApaI BamHI BanI BanII BbsI
BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BsaI
BsaHI BseRI BseYI BsmI Bsp1286I BspMI BsrDI BsrFI
BssSI BstXI BstYI BtgI BtgZI DrdI EaeI EcoNI
EcoO109I FalI HincII HindIII MmeI MscI MspA1I NcoI
NsiI NspI PflMI PspOMI PstI PvuII SalI SfcI
SphI StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BciVI BclI
BglII BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI ZraI