Restriction map for
pFN21AE2796
(Linear) (Six-base) MAPSORT of: KIEE2796.seq Check: 9309 from: 1 to: 1212
KIEE2796.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 479 1212
Size: 479 733
AflIII A'CryG_T
Cuts at: 0 520 561 1212
Size: 520 41 651
Fragments arranged by size:
651 520 41
AleI CACnn'nnGTG
Cuts at: 0 562 1212
Size: 562 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1208 1212
Size: 1208 4
AlwNI CAG_nnn'CTG
Cuts at: 0 390 1212
Size: 390 822
ApoI r'AATT_y
Cuts at: 0 637 1033 1212
Size: 637 396 179
Fragments arranged by size:
637 396 179
AvaI C'yCGr_G
Cuts at: 0 397 1212
Size: 397 815
BanI G'GyrC_C
Cuts at: 0 460 539 1212
Size: 460 79 673
Fragments arranged by size:
673 460 79
BanII G_rGCy'C
Cuts at: 0 89 1212
Size: 89 1123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 984 1018 1212
Size: 984 34 194
Fragments arranged by size:
984 194 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 641 1212
Size: 641 571
BclI T'GATC_A
Cuts at: 0 1055 1212
Size: 1055 157
BglI GCCn_nnn'nGGC
Cuts at: 0 22 1212
Size: 22 1190
Bme1580I G_kGCm'C
Cuts at: 0 397 544 1212
Size: 397 147 668
Fragments arranged by size:
668 397 147
BmgBI CAC'GTC
Cuts at: 0 521 1212
Size: 521 691
BmrI ACTGGGnnnn_n'
Cuts at: 0 831 1212
Size: 831 381
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 354 1212
Size: 354 858
Bpu10I CC'TnA_GC
Cuts at: 0 326 1212
Size: 326 886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1212
Size: 673 539
BsaI GGTCTCn'nnnn_
Cuts at: 0 944 1212
Size: 944 268
BsaAI yAC'GTr
Cuts at: 0 564 1212
Size: 564 648
BsaBI GATnn'nnATC
Cuts at: 0 1060 1212
Size: 1060 152
BsaWI w'CCGG_w
Cuts at: 0 290 1212
Size: 290 922
BsiEI CG_ry'CG
Cuts at: 0 1097 1212
Size: 1097 115
BsiHKAI G_wGCw'C
Cuts at: 0 65 905 1212
Size: 65 840 307
Fragments arranged by size:
840 307 65
Bsp1286I G_dGCh'C
Cuts at: 0 65 89 397 544 905 1212
Size: 65 24 308 147 361 307
Fragments arranged by size:
361 308 307 147 65 24
BspEI T'CCGG_A
Cuts at: 0 290 1212
Size: 290 922
BspHI T'CATG_A
Cuts at: 0 89 350 1212
Size: 89 261 862
Fragments arranged by size:
862 261 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 479 582 1212
Size: 479 103 630
Fragments arranged by size:
630 479 103
BsrFI r'CCGG_y
Cuts at: 0 448 457 1212
Size: 448 9 755
Fragments arranged by size:
755 448 9
BstYI r'GATC_y
Cuts at: 0 293 717 1212
Size: 293 424 495
Fragments arranged by size:
495 424 293
BtgI C'CryG_G
Cuts at: 0 224 788 1212
Size: 224 564 424
Fragments arranged by size:
564 424 224
BtsI GCAGTG_nn'
Cuts at: 0 1078 1212
Size: 1078 134
DraI TTT'AAA
Cuts at: 0 584 1212
Size: 584 628
DraIII CAC_nnn'GTG
Cuts at: 0 422 1212
Size: 422 790
DrdI GACnn_nn'nnGTC
Cuts at: 0 226 1114 1212
Size: 226 888 98
Fragments arranged by size:
888 226 98
EaeI y'GGCC_r
Cuts at: 0 785 1212
Size: 785 427
EarI CTCTTCn'nnn_
Cuts at: 0 349 1212
Size: 349 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 322 865 1212
Size: 322 543 347
Fragments arranged by size:
543 347 322
EcoO109I rG'GnC_Cy
Cuts at: 0 387 1212
Size: 387 825
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 309 310 341 1212
Size: 278 31 1 31 871
Fragments arranged by size:
871 278 31 31 1
HincII GTy'rAC
Cuts at: 0 819 1212
Size: 819 393
MslI CAynn'nnrTG
Cuts at: 0 562 787 1012 1212
Size: 562 225 225 200
Fragments arranged by size:
562 225 225 200
MspA1I CmG'CkG
Cuts at: 0 440 1212
Size: 440 772
NaeI GCC'GGC
Cuts at: 0 450 459 1212
Size: 450 9 753
Fragments arranged by size:
753 450 9
NgoMIV G'CCGG_C
Cuts at: 0 448 457 1212
Size: 448 9 755
Fragments arranged by size:
755 448 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 783 1212
Size: 783 429
PfoI T'CCnGG_A
Cuts at: 0 776 1212
Size: 776 436
PmlI CAC'GTG
Cuts at: 0 564 1212
Size: 564 648
PshAI GACnn'nnGTC
Cuts at: 0 247 1212
Size: 247 965
PstI C_TGCA'G
Cuts at: 0 594 1212
Size: 594 618
PvuII CAG'CTG
Cuts at: 0 440 1212
Size: 440 772
ScaI AGT'ACT
Cuts at: 0 943 1212
Size: 943 269
SfcI C'TryA_G
Cuts at: 0 590 945 1212
Size: 590 355 267
Fragments arranged by size:
590 355 267
SmlI C'TyrA_G
Cuts at: 0 688 1212
Size: 688 524
StuI AGG'CCT
Cuts at: 0 988 1212
Size: 988 224
StyI C'CwwG_G
Cuts at: 0 245 1212
Size: 245 967
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 478 1212
Size: 174 304 734
Fragments arranged by size:
734 304 174
TatI w'GTAC_w
Cuts at: 0 442 941 1212
Size: 442 499 271
Fragments arranged by size:
499 442 271
Enzymes that do cut:
AarI AflIII AleI AlfI AlwNI ApoI AvaI BanI
BanII BcgI BciVI BclI BglI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrFI BstYI BtgI
BtsI DraI DraIII DrdI EaeI EarI EciI EcoO109I
Hin4I HincII MslI MspA1I NaeI NgoMIV PflMI PfoI
PmlI PshAI PstI PvuII ScaI SfcI SmlI StuI
StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BfrBI BglII BlpI
BmtI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiWI
BsmI BsmBI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
EagI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI