Restriction map for
pFN21AE2795
(Linear) (Six-base) MAPSORT of: KIEE2795.seq Check: 247 from: 1 to: 1191
KIEE2795.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AccI GT'mk_AC
Cuts at: 0 926 1191
Size: 926 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 913 1191
Size: 822 91 278
Fragments arranged by size:
822 278 91
AfeI AGC'GCT
Cuts at: 0 376 1191
Size: 376 815
AleI CACnn'nnGTG
Cuts at: 0 466 1191
Size: 466 725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 701 1191
Size: 701 490
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1191
Size: 27 1164
AlwNI CAG_nnn'CTG
Cuts at: 0 96 1191
Size: 96 1095
BamHI G'GATC_C
Cuts at: 0 392 1191
Size: 392 799
BanI G'GyrC_C
Cuts at: 0 256 455 1191
Size: 256 199 736
Fragments arranged by size:
736 256 199
BanII G_rGCy'C
Cuts at: 0 199 1191
Size: 199 992
BbsI GAAGACnn'nnnn_
Cuts at: 0 607 1191
Size: 607 584
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 708 742 894 928 1191
Size: 708 34 152 34 263
Fragments arranged by size:
708 263 152 34 34
BclI T'GATC_A
Cuts at: 0 98 1191
Size: 98 1093
BglI GCCn_nnn'nGGC
Cuts at: 0 694 1191
Size: 694 497
Bme1580I G_kGCm'C
Cuts at: 0 259 460 1191
Size: 259 201 731
Fragments arranged by size:
731 259 201
BmgBI CAC'GTC
Cuts at: 0 660 918 1191
Size: 660 258 273
Fragments arranged by size:
660 273 258
BmrI ACTGGGnnnn_n'
Cuts at: 0 1114 1191
Size: 1114 77
Bpu10I CC'TnA_GC
Cuts at: 0 371 1191
Size: 371 820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 1191
Size: 1024 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 1191
Size: 25 1166
BsaAI yAC'GTr
Cuts at: 0 240 1191
Size: 240 951
BsaHI Gr'CG_yC
Cuts at: 0 244 1191
Size: 244 947
BsaWI w'CCGG_w
Cuts at: 0 199 431 1191
Size: 199 232 760
Fragments arranged by size:
760 232 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1141 1171 1191
Size: 1141 30 20
Fragments arranged by size:
1141 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 1090 1191
Size: 346 744 101
Fragments arranged by size:
744 346 101
BseYI C'CCAG_C
Cuts at: 0 731 1129 1191
Size: 731 398 62
Fragments arranged by size:
731 398 62
BsiHKAI G_wGCw'C
Cuts at: 0 431 1191
Size: 431 760
BsmI GAATG_Cn'
Cuts at: 0 939 1191
Size: 939 252
Bsp1286I G_dGCh'C
Cuts at: 0 199 259 431 460 1191
Size: 199 60 172 29 731
Fragments arranged by size:
731 199 172 60 29
BspEI T'CCGG_A
Cuts at: 0 199 431 1191
Size: 199 232 760
Fragments arranged by size:
760 232 199
BspHI T'CATG_A
Cuts at: 0 491 1191
Size: 491 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 613 1191
Size: 613 578
BssHII G'CGCG_C
Cuts at: 0 697 1191
Size: 697 494
BssSI C'ACGA_G
Cuts at: 0 133 1191
Size: 133 1058
BstEII G'GTnAC_C
Cuts at: 0 180 1191
Size: 180 1011
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 468 495 1191
Size: 45 423 27 696
Fragments arranged by size:
696 423 45 27
BstYI r'GATC_y
Cuts at: 0 392 443 1191
Size: 392 51 748
Fragments arranged by size:
748 392 51
BstZ17I GTA'TAC
Cuts at: 0 927 1191
Size: 927 264
Bsu36I CC'TnA_GG
Cuts at: 0 251 1191
Size: 251 940
BtgI C'CryG_G
Cuts at: 0 461 1191
Size: 461 730
BtsI GCAGTG_nn'
Cuts at: 0 879 1191
Size: 879 312
DrdI GACnn_nn'nnGTC
Cuts at: 0 39 1191
Size: 39 1152
EaeI y'GGCC_r
Cuts at: 0 402 1191
Size: 402 789
EarI CTCTTCn'nnn_
Cuts at: 0 49 553 1191
Size: 49 504 638
Fragments arranged by size:
638 504 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 913 1191
Size: 822 91 278
Fragments arranged by size:
822 278 91
EcoNI CCTnn'n_nnAGG
Cuts at: 0 892 1191
Size: 892 299
EcoO109I rG'GnC_Cy
Cuts at: 0 627 1172 1191
Size: 627 545 19
Fragments arranged by size:
627 545 19
HaeII r_GCGC'y
Cuts at: 0 378 1191
Size: 378 813
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 1141 1173 1191
Size: 234 32 875 32 18
Fragments arranged by size:
875 234 32 32 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 1191
Size: 38 1153
MslI CAynn'nnrTG
Cuts at: 0 226 283 466 493 1191
Size: 226 57 183 27 698
Fragments arranged by size:
698 226 183 57 27
MspA1I CmG'CkG
Cuts at: 0 344 407 1147 1191
Size: 344 63 740 44
Fragments arranged by size:
740 344 63 44
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 1191
Size: 406 32 753
Fragments arranged by size:
753 406 32
PpuMI rG'GwC_Cy
Cuts at: 0 627 1191
Size: 627 564
SapI GCTCTTCn'nnn_
Cuts at: 0 49 1191
Size: 49 1142
ScaI AGT'ACT
Cuts at: 0 853 1191
Size: 853 338
SexAI A'CCwGG_T
Cuts at: 0 146 1191
Size: 146 1045
SfcI C'TryA_G
Cuts at: 0 417 1191
Size: 417 774
SmlI C'TyrA_G
Cuts at: 0 1039 1191
Size: 1039 152
StuI AGG'CCT
Cuts at: 0 754 1191
Size: 754 437
StyI C'CwwG_G
Cuts at: 0 185 1191
Size: 185 1006
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 252 723 1191
Size: 252 471 468
Fragments arranged by size:
471 468 252
TatI w'GTAC_w
Cuts at: 0 851 1055 1191
Size: 851 204 136
Fragments arranged by size:
851 204 136
Tth111I GACn'n_nGTC
Cuts at: 0 661 1191
Size: 661 530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 219 1191
Size: 42 177 972
Fragments arranged by size:
972 177 42
Enzymes that do cut:
AccI AcuI AfeI AleI AlfI AloI AlwNI BamHI
BanI BanII BbsI BcgI BclI BglI Bme1580I BmgBI
BmrI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BssHII BssSI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I MmeI MslI MspA1I PpiI PpuMI SapI ScaI
SexAI SfcI SmlI StuI StyI TaqII TatI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BciVI BfrBI BglII BlpI BmtI
BplI BpmI BsaBI BsgI BsiEI BsiWI BsmBI BsrBI
BsrDI BsrFI BsrGI BstAPI BstBI BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XhoI
XmaI XmnI ZraI