Restriction map for
pFN21AE2789
(Linear) (Six-base) MAPSORT of: KIEE2789.seq Check: 2836 from: 1 to: 681
KIEE2789.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 681
Size: 491 190
AlwNI CAG_nnn'CTG
Cuts at: 0 469 613 681
Size: 469 144 68
Fragments arranged by size:
469 144 68
ApaI G_GGCC'C
Cuts at: 0 501 604 681
Size: 501 103 77
Fragments arranged by size:
501 103 77
AvaI C'yCGr_G
Cuts at: 0 259 310 681
Size: 259 51 371
Fragments arranged by size:
371 259 51
BanI G'GyrC_C
Cuts at: 0 306 456 681
Size: 306 150 225
Fragments arranged by size:
306 225 150
BanII G_rGCy'C
Cuts at: 0 125 143 260 299 422 501 604 626
Size: 125 18 117 39 123 79 103 22
Cuts at: 626 681
Size: 55
Fragments arranged by size:
125 123 117 103 79 55 39 22
18
BbeI G_GCGC'C
Cuts at: 0 310 460 681
Size: 310 150 221
Fragments arranged by size:
310 221 150
BbsI GAAGACnn'nnnn_
Cuts at: 0 271 681
Size: 271 410
BbvCI CC'TCA_GC
Cuts at: 0 368 681
Size: 368 313
BglI GCCn_nnn'nGGC
Cuts at: 0 250 379 681
Size: 250 129 302
Fragments arranged by size:
302 250 129
BlpI GC'TnA_GC
Cuts at: 0 13 293 681
Size: 13 280 388
Fragments arranged by size:
388 280 13
Bme1580I G_kGCm'C
Cuts at: 0 501 604 681
Size: 501 103 77
Fragments arranged by size:
501 103 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 461 681
Size: 461 220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 28 681
Size: 28 653
Bpu10I CC'TnA_GC
Cuts at: 0 368 681
Size: 368 313
BsaI GGTCTCn'nnnn_
Cuts at: 0 475 547 681
Size: 475 72 134
Fragments arranged by size:
475 134 72
BsaHI Gr'CG_yC
Cuts at: 0 182 307 457 681
Size: 182 125 150 224
Fragments arranged by size:
224 182 150 125
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 214 253 430 681
Size: 112 102 39 177 251
Fragments arranged by size:
251 177 112 102 39
BseYI C'CCAG_C
Cuts at: 0 421 501 681
Size: 421 80 180
Fragments arranged by size:
421 180 80
BsiEI CG_ry'CG
Cuts at: 0 134 389 681
Size: 134 255 292
Fragments arranged by size:
292 255 134
Bsp1286I G_dGCh'C
Cuts at: 0 125 143 260 299 422 501 604 626
Size: 125 18 117 39 123 79 103 22
Cuts at: 626 681
Size: 55
Fragments arranged by size:
125 123 117 103 79 55 39 22
18
BsrFI r'CCGG_y
Cuts at: 0 630 681
Size: 630 51
BstAPI GCAn_nnn'nTGC
Cuts at: 0 469 613 681
Size: 469 144 68
Fragments arranged by size:
469 144 68
BstXI CCAn_nnnn'nTGG
Cuts at: 0 633 681
Size: 633 48
BtgI C'CryG_G
Cuts at: 0 377 566 681
Size: 377 189 115
Fragments arranged by size:
377 189 115
EaeI y'GGCC_r
Cuts at: 0 131 386 681
Size: 131 255 295
Fragments arranged by size:
295 255 131
EagI C'GGCC_G
Cuts at: 0 131 386 681
Size: 131 255 295
Fragments arranged by size:
295 255 131
EarI CTCTTCn'nnn_
Cuts at: 0 89 549 614 681
Size: 89 460 65 67
Fragments arranged by size:
460 89 67 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 268 681
Size: 268 413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 681
Size: 491 190
EcoO109I rG'GnC_Cy
Cuts at: 0 104 243 354 497 498 600 601 639
Size: 104 139 111 143 1 102 1 38
Cuts at: 639 681
Size: 42
Fragments arranged by size:
143 139 111 104 102 42 38 1
1
HaeII r_GCGC'y
Cuts at: 0 310 460 577 681
Size: 310 150 117 104
Fragments arranged by size:
310 150 117 104
KasI G'GCGC_C
Cuts at: 0 306 456 681
Size: 306 150 225
Fragments arranged by size:
306 225 150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 681
Size: 680 1
MspA1I CmG'CkG
Cuts at: 0 340 379 681
Size: 340 39 302
Fragments arranged by size:
340 302 39
NarI GG'CG_CC
Cuts at: 0 307 457 681
Size: 307 150 224
Fragments arranged by size:
307 224 150
NotI GC'GGCC_GC
Cuts at: 0 131 681
Size: 131 550
PasI CC'CwG_GG
Cuts at: 0 597 681
Size: 597 84
PpuMI rG'GwC_Cy
Cuts at: 0 104 681
Size: 104 577
PspOMI G'GGCC_C
Cuts at: 0 497 600 681
Size: 497 103 81
Fragments arranged by size:
497 103 81
PstI C_TGCA'G
Cuts at: 0 618 681
Size: 618 63
SacII CC_GC'GG
Cuts at: 0 380 681
Size: 380 301
SanDI GG'GwC_CC
Cuts at: 0 104 681
Size: 104 577
SapI GCTCTTCn'nnn_
Cuts at: 0 614 681
Size: 614 67
SfcI C'TryA_G
Cuts at: 0 614 681
Size: 614 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 379 681
Size: 379 302
SfoI GGC'GCC
Cuts at: 0 308 458 681
Size: 308 150 223
Fragments arranged by size:
308 223 150
StyI C'CwwG_G
Cuts at: 0 56 152 233 287 548 590 681
Size: 56 96 81 54 261 42 91
Fragments arranged by size:
261 96 91 81 56 54 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 603 681
Size: 603 78
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BmrI BplI Bpu10I BsaI
BsaHI BseRI BseYI BsiEI Bsp1286I BsrFI BstAPI BstXI
BtgI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII
KasI MmeI MspA1I NarI NotI PasI PpuMI PspOMI
PstI SacII SanDI SapI SfcI SfiI SfoI StyI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI BpmI BpuEI BsaAI BsaBI
BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SbfI ScaI SexAI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI