(Linear) (Six-base) MAPSORT of: KIEE2785.seq  Check: 1078  from: 1  to: 594



KIEE2785.orf

 With 182 enzymes: SgfI * 

                        February 19, 2021 20:23  ..

AatII G_ACGT'C

Cuts at:      0     389     594 
   Size:        389     205
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     594 
   Size:        182     412
 
AlwNI CAG_nnn'CTG

Cuts at:      0     551     594 
   Size:        551      43
 
BanII G_rGCy'C

Cuts at:      0     149     594 
   Size:        149     445
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      34     170     493     594 
   Size:         34     136     323     101

  Fragments arranged by size: 

                 323     136     101      34
 
Bme1580I G_kGCm'C

Cuts at:      0     128     594 
   Size:        128     466
 
BmgBI CAC'GTC

Cuts at:      0      57     177     594 
   Size:         57     120     417

  Fragments arranged by size: 

                 417     120      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     594 
   Size:        350     244
 
BsaHI Gr'CG_yC

Cuts at:      0      31     386     594 
   Size:         31     355     208

  Fragments arranged by size: 

                 355     208      31
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      73     103     448     478     594 
   Size:         73      30     345      30     116

  Fragments arranged by size: 

                 345     116      73      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61      94     594 
   Size:         61      33     500

  Fragments arranged by size: 

                 500      61      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     285     594 
   Size:         79     206     309

  Fragments arranged by size: 

                 309     206      79
 
BsiHKAI G_wGCw'C

Cuts at:      0     176     594 
   Size:        176     418
 
BsmI GAATG_Cn'

Cuts at:      0     520     594 
   Size:        520      74
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     149     176     594 
   Size:        128      21      27     418

  Fragments arranged by size: 

                 418     128      27      21
 
BssHII G'CGCG_C

Cuts at:      0      68     594 
   Size:         68     526
 
BstYI r'GATC_y

Cuts at:      0     281     594 
   Size:        281     313
 
BtsI GCAGTG_nn'

Cuts at:      0     273     361     594 
   Size:        273      88     233

  Fragments arranged by size: 

                 273     233      88
 
DraI TTT'AAA

Cuts at:      0     204     594 
   Size:        204     390
 
EarI CTCTTCn'nnn_

Cuts at:      0      58     164     594 
   Size:         58     106     430

  Fragments arranged by size: 

                 430     106      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     294     550     594 
   Size:        294     256      44

  Fragments arranged by size: 

                 294     256      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     350     594 
   Size:        182     168     244

  Fragments arranged by size: 

                 244     182     168
 
FspI TGC'GCA

Cuts at:      0     353     594 
   Size:        353     241
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      73     105     594 
   Size:         73      32     489

  Fragments arranged by size: 

                 489      73      32
 
PstI C_TGCA'G

Cuts at:      0      15     594 
   Size:         15     579
 
SapI GCTCTTCn'nnn_

Cuts at:      0      58     164     594 
   Size:         58     106     430

  Fragments arranged by size: 

                 430     106      58
 
SbfI CC_TGCA'GG

Cuts at:      0      15     594 
   Size:         15     579
 
SfcI C'TryA_G

Cuts at:      0      11     543     594 
   Size:         11     532      51

  Fragments arranged by size: 

                 532      51      11
 
StuI AGG'CCT

Cuts at:      0     367     594 
   Size:        367     227
 
StyI C'CwwG_G

Cuts at:      0      40     594 
   Size:         40     554
 
XmnI GAAnn'nnTTC

Cuts at:      0     340     367     424     594 
   Size:        340      27      57     170

  Fragments arranged by size: 

                 340     170      57      27
 
ZraI GAC'GTC

Cuts at:      0     387     594 
   Size:        387     207
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI    BanII     BbsI Bme1580I    BmgBI     BpmI
    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I   BssHII
    BstYI     BtsI     DraI     EarI     EciI  Eco57MI     FspI    Hin4I
     PstI     SapI     SbfI     SfcI     StuI     StyI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI