Restriction map for
pFN21AE2784
(Linear) (Six-base) MAPSORT of: KIEE2784.seq Check: 4480 from: 1 to: 975
KIEE2784.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 596 640 975
Size: 188 408 44 335
Fragments arranged by size:
408 335 188 44
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 63 95 165 197 975
Size: 63 32 70 32 778
Fragments arranged by size:
778 70 63 32 32
ApoI r'AATT_y
Cuts at: 0 256 414 457 531 723 892 937 975
Size: 256 158 43 74 192 169 45 38
Fragments arranged by size:
256 192 169 158 74 45 43 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 124 145 617 975
Size: 124 21 472 358
Fragments arranged by size:
472 358 124 21
BclI T'GATC_A
Cuts at: 0 291 975
Size: 291 684
BsaAI yAC'GTr
Cuts at: 0 439 975
Size: 439 536
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 44 651 681 695 725 975
Size: 14 30 607 30 14 30 250
Fragments arranged by size:
607 250 30 30 30 14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 314 317 320 393 975
Size: 314 3 3 73 582
Fragments arranged by size:
582 314 73 3 3
BsmI GAATG_Cn'
Cuts at: 0 956 975
Size: 956 19
BsrDI GCAATG_nn'
Cuts at: 0 260 975
Size: 260 715
BstBI TT'CG_AA
Cuts at: 0 960 975
Size: 960 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 236 907 975
Size: 236 671 68
Fragments arranged by size:
671 236 68
BstYI r'GATC_y
Cuts at: 0 556 975
Size: 556 419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 45 80 291 326 722 757 975
Size: 45 35 211 35 396 35 218
Fragments arranged by size:
396 218 211 45 35 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 8 975
Size: 8 967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 596 640 975
Size: 188 408 44 335
Fragments arranged by size:
408 335 188 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 292 304 698 758 975
Size: 292 12 394 60 217
Fragments arranged by size:
394 292 217 60 12
EcoO109I rG'GnC_Cy
Cuts at: 0 297 975
Size: 297 678
EcoRI G'AATT_C
Cuts at: 0 256 975
Size: 256 719
HaeII r_GCGC'y
Cuts at: 0 587 975
Size: 587 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 651 683 695 727 814 846
Size: 14 32 605 32 12 32 87 32
Cuts at: 846 975
Size: 129
Fragments arranged by size:
605 129 87 32 32 32 32 14
12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 208 975
Size: 18 190 767
Fragments arranged by size:
767 190 18
MslI CAynn'nnrTG
Cuts at: 0 905 975
Size: 905 70
MspA1I CmG'CkG
Cuts at: 0 400 975
Size: 400 575
PfoI T'CCnGG_A
Cuts at: 0 537 975
Size: 537 438
PvuII CAG'CTG
Cuts at: 0 400 975
Size: 400 575
SfcI C'TryA_G
Cuts at: 0 464 923 975
Size: 464 459 52
Fragments arranged by size:
464 459 52
SspI AAT'ATT
Cuts at: 0 852 975
Size: 852 123
StuI AGG'CCT
Cuts at: 0 287 975
Size: 287 688
Tth111I GACn'n_nGTC
Cuts at: 0 663 975
Size: 663 312
Enzymes that do cut:
AcuI AloI ApoI BbsI BclI BsaAI BsaXI BseRI
BsmI BsrDI BstBI BstXI BstYI CspCI EarI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I MmeI MslI MspA1I
PfoI PvuII SfcI SspI StuI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StyI SwaI TaqII TatI TstI
XbaI XcmI XhoI XmaI XmnI ZraI