Restriction map for
pFN21AE2782
(Linear) (Six-base) MAPSORT of: KIEE2782.seq Check: 1796 from: 1 to: 1350
KIEE2782.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AccI GT'mk_AC
Cuts at: 0 344 1350
Size: 344 1006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 172 708 978 1350
Size: 168 4 536 270 372
Fragments arranged by size:
536 372 270 168 4
AleI CACnn'nnGTG
Cuts at: 0 625 1042 1350
Size: 625 417 308
Fragments arranged by size:
625 417 308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 706 1350
Size: 706 644
AlwNI CAG_nnn'CTG
Cuts at: 0 39 1088 1307 1350
Size: 39 1049 219 43
Fragments arranged by size:
1049 219 43 39
ApaI G_GGCC'C
Cuts at: 0 506 634 1350
Size: 506 128 716
Fragments arranged by size:
716 506 128
ApoI r'AATT_y
Cuts at: 0 22 1350
Size: 22 1328
AvaI C'yCGr_G
Cuts at: 0 133 1350
Size: 133 1217
BamHI G'GATC_C
Cuts at: 0 1076 1350
Size: 1076 274
BanI G'GyrC_C
Cuts at: 0 444 487 1106 1350
Size: 444 43 619 244
Fragments arranged by size:
619 444 244 43
BanII G_rGCy'C
Cuts at: 0 506 634 1179 1210 1292 1350
Size: 506 128 545 31 82 58
Fragments arranged by size:
545 506 128 82 58 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 282 489 1350
Size: 282 207 861
Fragments arranged by size:
861 282 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1114 1148 1350
Size: 1114 34 202
Fragments arranged by size:
1114 202 34
BglI GCCn_nnn'nGGC
Cuts at: 0 397 769 1350
Size: 397 372 581
Fragments arranged by size:
581 397 372
BglII A'GATC_T
Cuts at: 0 83 1350
Size: 83 1267
BlpI GC'TnA_GC
Cuts at: 0 232 1291 1350
Size: 232 1059 59
Fragments arranged by size:
1059 232 59
Bme1580I G_kGCm'C
Cuts at: 0 378 449 490 506 634 1111 1350
Size: 378 71 41 16 128 477 239
Fragments arranged by size:
477 378 239 128 71 41 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1317 1350
Size: 1317 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1350
Size: 147 1203
BsaAI yAC'GTr
Cuts at: 0 1044 1350
Size: 1044 306
BsaHI Gr'CG_yC
Cuts at: 0 287 1350
Size: 287 1063
BsaWI w'CCGG_w
Cuts at: 0 1058 1350
Size: 1058 292
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1192 1222 1350
Size: 1192 30 128
Fragments arranged by size:
1192 128 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 805 1258 1350
Size: 112 693 453 92
Fragments arranged by size:
693 453 112 92
BseYI C'CCAG_C
Cuts at: 0 65 1295 1350
Size: 65 1230 55
Fragments arranged by size:
1230 65 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1350
Size: 193 1157
BsiHKAI G_wGCw'C
Cuts at: 0 1179 1292 1350
Size: 1179 113 58
Fragments arranged by size:
1179 113 58
BsmI GAATG_Cn'
Cuts at: 0 930 1350
Size: 930 420
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1345 1350
Size: 1345 5
Bsp1286I G_dGCh'C
Cuts at: 0 378 449 490 506 634 1111 1179 1210
Size: 378 71 41 16 128 477 68 31
Cuts at: 1210 1292 1350
Size: 82 58
Fragments arranged by size:
477 378 128 82 71 68 58 41
31 16
BspEI T'CCGG_A
Cuts at: 0 1058 1350
Size: 1058 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 893 1350
Size: 893 457
BsrFI r'CCGG_y
Cuts at: 0 560 1350
Size: 560 790
BssSI C'ACGA_G
Cuts at: 0 250 1350
Size: 250 1100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 39 366 1350
Size: 39 327 984
Fragments arranged by size:
984 327 39
BstEII G'GTnAC_C
Cuts at: 0 667 960 1350
Size: 667 293 390
Fragments arranged by size:
667 390 293
BstXI CCAn_nnnn'nTGG
Cuts at: 0 627 1350
Size: 627 723
BstYI r'GATC_y
Cuts at: 0 83 1076 1328 1350
Size: 83 993 252 22
Fragments arranged by size:
993 252 83 22
BtgI C'CryG_G
Cuts at: 0 167 758 1350
Size: 167 591 592
Fragments arranged by size:
592 591 167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1117 1350
Size: 1117 233
BtsI GCAGTG_nn'
Cuts at: 0 407 1350
Size: 407 943
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 149 184 1350
Size: 149 35 1166
Fragments arranged by size:
1166 149 35
EaeI y'GGCC_r
Cuts at: 0 2 389 761 1181 1350
Size: 2 387 372 420 169
Fragments arranged by size:
420 387 372 169 2
EarI CTCTTCn'nnn_
Cuts at: 0 646 1350
Size: 646 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 922 1350
Size: 922 428
EcoICRI GAG'CTC
Cuts at: 0 1177 1290 1350
Size: 1177 113 60
Fragments arranged by size:
1177 113 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 168 172 708 978 1350
Size: 147 21 4 536 270 372
Fragments arranged by size:
536 372 270 147 21 4
EcoO109I rG'GnC_Cy
Cuts at: 0 72 503 631 813 941 1350
Size: 72 431 128 182 128 409
Fragments arranged by size:
431 409 182 128 128 72
EcoRV GAT'ATC
Cuts at: 0 512 1350
Size: 512 838
HaeII r_GCGC'y
Cuts at: 0 980 1068 1350
Size: 980 88 282
Fragments arranged by size:
980 282 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1313 1345 1350
Size: 1313 32 5
Fragments arranged by size:
1313 32 5
MscI TGG'CCA
Cuts at: 0 4 391 1183 1350
Size: 4 387 792 167
Fragments arranged by size:
792 387 167 4
MslI CAynn'nnrTG
Cuts at: 0 625 1042 1350
Size: 625 417 308
Fragments arranged by size:
625 417 308
MspA1I CmG'CkG
Cuts at: 0 207 1295 1350
Size: 207 1088 55
Fragments arranged by size:
1088 207 55
NspI r_CATG'y
Cuts at: 0 160 1054 1350
Size: 160 894 296
Fragments arranged by size:
894 296 160
PflMI CCAn_nnn'nTGG
Cuts at: 0 1307 1350
Size: 1307 43
PpuMI rG'GwC_Cy
Cuts at: 0 813 941 1350
Size: 813 128 409
Fragments arranged by size:
813 409 128
PspOMI G'GGCC_C
Cuts at: 0 502 630 1350
Size: 502 128 720
Fragments arranged by size:
720 502 128
PstI C_TGCA'G
Cuts at: 0 572 1350
Size: 572 778
PvuII CAG'CTG
Cuts at: 0 207 1295 1350
Size: 207 1088 55
Fragments arranged by size:
1088 207 55
SacI G_AGCT'C
Cuts at: 0 1179 1292 1350
Size: 1179 113 58
Fragments arranged by size:
1179 113 58
SfcI C'TryA_G
Cuts at: 0 568 1350
Size: 568 782
SphI G_CATG'C
Cuts at: 0 160 1350
Size: 160 1190
StyI C'CwwG_G
Cuts at: 0 540 1350
Size: 540 810
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 842 1350
Size: 316 526 508
Fragments arranged by size:
526 508 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1350
Size: 1068 32 250
Fragments arranged by size:
1068 250 32
Tth111I GACn'n_nGTC
Cuts at: 0 822 1350
Size: 822 528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 1191 1350
Size: 765 426 159
Fragments arranged by size:
765 426 159
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BcgI BglI BglII BlpI
Bme1580I BmrI BpmI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV HaeII Hin4I MscI MslI MspA1I NspI PflMI
PpuMI PspOMI PstI PvuII SacI SfcI SphI StyI
TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BclI BfrBI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BspHI BsrBI
BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI DraI
DraIII DrdI EagI EcoNI EcoRI FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TatI XbaI XhoI XmaI XmnI ZraI