(Linear) (Six-base) MAPSORT of: KIEE2781.seq  Check: 2487  from: 1  to: 378



KIEE2781.orf

 With 182 enzymes: SgfI * 

                        February 19, 2021 20:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     132     378 
   Size:        132     246
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     378 
   Size:        157     221
 
AfeI AGC'GCT

Cuts at:      0     259     378 
   Size:        259     119
 
AflIII A'CryG_T

Cuts at:      0     122     378 
   Size:        122     256
 
ApaLI G'TGCA_C

Cuts at:      0     145     378 
   Size:        145     233
 
BanI G'GyrC_C

Cuts at:      0     343     378 
   Size:        343      35
 
BglI GCCn_nnn'nGGC

Cuts at:      0     229     378 
   Size:        229     149
 
Bme1580I G_kGCm'C

Cuts at:      0     149     346     378 
   Size:        149     197      32

  Fragments arranged by size: 

                 197     149      32
 
BmgBI CAC'GTC

Cuts at:      0     272     378 
   Size:        272     106
 
BsaAI yAC'GTr

Cuts at:      0     123     378 
   Size:        123     255
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     244     274     355     378 
   Size:        244      30      81      23

  Fragments arranged by size: 

                 244      81      30      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     378 
   Size:        304      74
 
BsiEI CG_ry'CG

Cuts at:      0      99     293     378 
   Size:         99     194      85

  Fragments arranged by size: 

                 194      99      85
 
BsiHKAI G_wGCw'C

Cuts at:      0     149     378 
   Size:        149     229
 
Bsp1286I G_dGCh'C

Cuts at:      0     149     346     378 
   Size:        149     197      32

  Fragments arranged by size: 

                 197     149      32
 
BspHI T'CATG_A

Cuts at:      0     185     378 
   Size:        185     193
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     132     378 
   Size:        132     246
 
BsrFI r'CCGG_y

Cuts at:      0     157     378 
   Size:        157     221
 
BsrGI T'GTAC_A

Cuts at:      0     125     378 
   Size:        125     253
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177     378 
   Size:        177     201
 
BtgI C'CryG_G

Cuts at:      0     176     378 
   Size:        176     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     204     378 
   Size:        204     174
 
DraIII CAC_nnn'GTG

Cuts at:      0     333     378 
   Size:        333      45
 
EaeI y'GGCC_r

Cuts at:      0      96     290     320     378 
   Size:         96     194      30      58

  Fragments arranged by size: 

                 194      96      58      30
 
EagI C'GGCC_G

Cuts at:      0      96     290     378 
   Size:         96     194      88

  Fragments arranged by size: 

                 194      96      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     378 
   Size:        157     221
 
HaeII r_GCGC'y

Cuts at:      0     261     378 
   Size:        261     117
 
MscI TGG'CCA

Cuts at:      0     322     378 
   Size:        322      56
 
NcoI C'CATG_G

Cuts at:      0     176     378 
   Size:        176     202
 
NotI GC'GGCC_GC

Cuts at:      0      96     378 
   Size:         96     282
 
StuI AGG'CCT

Cuts at:      0     232     378 
   Size:        232     146
 
StyI C'CwwG_G

Cuts at:      0      59     170     176     347     378 
   Size:         59     111       6     171      31

  Fragments arranged by size: 

                 171     111      59      31       6
 
TatI w'GTAC_w

Cuts at:      0     125     362     378 
   Size:        125     237      16

  Fragments arranged by size: 

                 237     125      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     319     351     378 
   Size:        319      32      27

  Fragments arranged by size: 

                 319      32      27
 
XmnI GAAnn'nnTTC

Cuts at:      0     193     378 
   Size:        193     185
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII    ApaLI     BanI     BglI Bme1580I
    BmgBI    BsaAI    BsaXI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrFI    BsrGI    BstXI     BtgI    BtgZI   DraIII     EaeI
     EagI  Eco57MI    HaeII     MscI     NcoI     NotI     StuI     StyI
     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI     DrdI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI