Restriction map for
pFN21AE2780
(Linear) (Six-base) MAPSORT of: KIEE2780.seq Check: 5673 from: 1 to: 2424
KIEE2780.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
Acc65I G'GTAC_C
Cuts at: 0 700 2424
Size: 700 1724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 2050 2105 2183 2347 2424
Size: 153 1897 55 78 164 77
Fragments arranged by size:
1897 164 153 78 77 55
AfeI AGC'GCT
Cuts at: 0 343 1522 2424
Size: 343 1179 902
Fragments arranged by size:
1179 902 343
AleI CACnn'nnGTG
Cuts at: 0 2353 2424
Size: 2353 71
AlwNI CAG_nnn'CTG
Cuts at: 0 2272 2424
Size: 2272 152
ApoI r'AATT_y
Cuts at: 0 1407 1649 2128 2424
Size: 1407 242 479 296
Fragments arranged by size:
1407 479 296 242
AseI AT'TA_AT
Cuts at: 0 509 1279 1939 2424
Size: 509 770 660 485
Fragments arranged by size:
770 660 509 485
BamHI G'GATC_C
Cuts at: 0 388 2424
Size: 388 2036
BanI G'GyrC_C
Cuts at: 0 151 700 1218 2424
Size: 151 549 518 1206
Fragments arranged by size:
1206 549 518 151
BanII G_rGCy'C
Cuts at: 0 1571 2424
Size: 1571 853
BbsI GAAGACnn'nnnn_
Cuts at: 0 1368 2056 2351 2403 2424
Size: 1368 688 295 52 21
Fragments arranged by size:
1368 688 295 52 21
BbvCI CC'TCA_GC
Cuts at: 0 1537 2424
Size: 1537 887
BclI T'GATC_A
Cuts at: 0 1083 1911 2424
Size: 1083 828 513
Fragments arranged by size:
1083 828 513
BfrBI ATG'CAT
Cuts at: 0 1234 1415 2424
Size: 1234 181 1009
Fragments arranged by size:
1234 1009 181
BglII A'GATC_T
Cuts at: 0 80 2424
Size: 80 2344
Bme1580I G_kGCm'C
Cuts at: 0 135 154 2424
Size: 135 19 2270
Fragments arranged by size:
2270 135 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1604 2321 2424
Size: 910 694 717 103
Fragments arranged by size:
910 717 694 103
Bpu10I CC'TnA_GC
Cuts at: 0 1537 2424
Size: 1537 887
BsaHI Gr'CG_yC
Cuts at: 0 353 2424
Size: 353 2071
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 866 896 941 971 1431 1461 2207 2237
Size: 866 30 45 30 460 30 746 30
Cuts at: 2237 2424
Size: 187
Fragments arranged by size:
866 746 460 187 45 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 965 1870 2424
Size: 385 580 905 554
Fragments arranged by size:
905 580 554 385
BseYI C'CCAG_C
Cuts at: 0 276 2424
Size: 276 2148
BsiHKAI G_wGCw'C
Cuts at: 0 627 1571 2424
Size: 627 944 853
Fragments arranged by size:
944 853 627
BsmI GAATG_Cn'
Cuts at: 0 492 2424
Size: 492 1932
BsmBI CGTCTCn'nnnn_
Cuts at: 0 788 958 2424
Size: 788 170 1466
Fragments arranged by size:
1466 788 170
Bsp1286I G_dGCh'C
Cuts at: 0 135 154 627 1571 2424
Size: 135 19 473 944 853
Fragments arranged by size:
944 853 473 135 19
BspHI T'CATG_A
Cuts at: 0 1841 2424
Size: 1841 583
BssSI C'ACGA_G
Cuts at: 0 848 2246 2424
Size: 848 1398 178
Fragments arranged by size:
1398 848 178
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 2031 2424
Size: 304 1727 393
Fragments arranged by size:
1727 393 304
BstYI r'GATC_y
Cuts at: 0 80 388 1155 2389 2424
Size: 80 308 767 1234 35
Fragments arranged by size:
1234 767 308 80 35
BtgI C'CryG_G
Cuts at: 0 636 2172 2424
Size: 636 1536 252
Fragments arranged by size:
1536 636 252
DraI TTT'AAA
Cuts at: 0 1754 2424
Size: 1754 670
DraIII CAC_nnn'GTG
Cuts at: 0 853 2424
Size: 853 1571
EarI CTCTTCn'nnn_
Cuts at: 0 786 1679 2424
Size: 786 893 745
Fragments arranged by size:
893 786 745
EcoICRI GAG'CTC
Cuts at: 0 1569 2424
Size: 1569 855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 910 1604 2050 2105 2183 2321 2347
Size: 153 757 694 446 55 78 138 26
Cuts at: 2347 2424
Size: 77
Fragments arranged by size:
757 694 446 153 138 78 77 55
26
EcoO109I rG'GnC_Cy
Cuts at: 0 783 1089 2089 2269 2424
Size: 783 306 1000 180 155
Fragments arranged by size:
1000 783 306 180 155
EcoRI G'AATT_C
Cuts at: 0 2128 2424
Size: 2128 296
EcoRV GAT'ATC
Cuts at: 0 444 2424
Size: 444 1980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 408 754 1333 2080 2424
Size: 183 225 346 579 747 344
Fragments arranged by size:
747 579 346 344 225 183
HaeII r_GCGC'y
Cuts at: 0 345 1269 1524 2424
Size: 345 924 255 900
Fragments arranged by size:
924 900 345 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 727 759 941 973 2424
Size: 72 32 623 32 182 32 1451
Fragments arranged by size:
1451 623 182 72 32 32 32
HincII GTy'rAC
Cuts at: 0 48 524 2424
Size: 48 476 1900
Fragments arranged by size:
1900 476 48
HpaI GTT'AAC
Cuts at: 0 48 2424
Size: 48 2376
KpnI G_GTAC'C
Cuts at: 0 704 2424
Size: 704 1720
MfeI C'AATT_G
Cuts at: 0 983 2424
Size: 983 1441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1884 2424
Size: 1884 540
MslI CAynn'nnrTG
Cuts at: 0 302 2353 2424
Size: 302 2051 71
Fragments arranged by size:
2051 302 71
MspA1I CmG'CkG
Cuts at: 0 757 1393 2424
Size: 757 636 1031
Fragments arranged by size:
1031 757 636
NcoI C'CATG_G
Cuts at: 0 636 2172 2424
Size: 636 1536 252
Fragments arranged by size:
1536 636 252
NsiI A_TGCA'T
Cuts at: 0 1236 1417 2424
Size: 1236 181 1007
Fragments arranged by size:
1236 1007 181
PpuMI rG'GwC_Cy
Cuts at: 0 1089 2089 2269 2424
Size: 1089 1000 180 155
Fragments arranged by size:
1089 1000 180 155
PsiI TTA'TAA
Cuts at: 0 2020 2424
Size: 2020 404
PstI C_TGCA'G
Cuts at: 0 1289 1557 2424
Size: 1289 268 867
Fragments arranged by size:
1289 867 268
PvuII CAG'CTG
Cuts at: 0 757 1393 2424
Size: 757 636 1031
Fragments arranged by size:
1031 757 636
SacI G_AGCT'C
Cuts at: 0 1571 2424
Size: 1571 853
SapI GCTCTTCn'nnn_
Cuts at: 0 1679 2424
Size: 1679 745
SexAI A'CCwGG_T
Cuts at: 0 1734 2424
Size: 1734 690
SfcI C'TryA_G
Cuts at: 0 1285 1553 2424
Size: 1285 268 871
Fragments arranged by size:
1285 871 268
SpeI A'CTAG_T
Cuts at: 0 1633 2424
Size: 1633 791
StyI C'CwwG_G
Cuts at: 0 114 527 636 786 2172 2424
Size: 114 413 109 150 1386 252
Fragments arranged by size:
1386 413 252 150 114 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 866 871 898 1431 1463 2207 2239
Size: 839 27 5 27 533 32 744 32
Cuts at: 2239 2424
Size: 185
Fragments arranged by size:
839 744 533 185 32 32 27 27
5
XbaI T'CTAG_A
Cuts at: 0 2392 2424
Size: 2392 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 598 2424
Size: 598 1826
XmnI GAAnn'nnTTC
Cuts at: 0 166 2424
Size: 166 2258
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI
BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BssSI BstXI BstYI BtgI DraI
DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HpaI KpnI MfeI MmeI MslI
MspA1I NcoI NsiI PpuMI PsiI PstI PvuII SacI
SapI SexAI SfcI SpeI StyI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DrdI EaeI EagI EciI EcoNI FseI
FspI FspAI HindIII KasI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XhoI XmaI ZraI