(Linear) (Six-base) MAPSORT of: KIEE2771.seq  Check: 4612  from: 1  to: 1527



KIEE2771.orf

 With 182 enzymes: SgfI * 

                        July 3, 2020 20:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234    1527 
   Size:        234    1293
 
AfeI AGC'GCT

Cuts at:      0     416    1527 
   Size:        416    1111
 
AlwNI CAG_nnn'CTG

Cuts at:      0     212    1527 
   Size:        212    1315
 
AseI AT'TA_AT

Cuts at:      0    1106    1303    1527 
   Size:       1106     197     224

  Fragments arranged by size: 

                1106     224     197
 
AvrII C'CTAG_G

Cuts at:      0    1212    1332    1527 
   Size:       1212     120     195

  Fragments arranged by size: 

                1212     195     120
 
BamHI G'GATC_C

Cuts at:      0      82     163    1208    1527 
   Size:         82      81    1045     319

  Fragments arranged by size: 

                1045     319      82      81
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1267    1527 
   Size:       1267     260
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1076    1452    1527 
   Size:       1076     376      75

  Fragments arranged by size: 

                1076     376      75
 
BglII A'GATC_T

Cuts at:      0     615    1527 
   Size:        615     912
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     593    1527 
   Size:        290     303     934

  Fragments arranged by size: 

                 934     303     290
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     558    1527 
   Size:        558     969
 
BsaAI yAC'GTr

Cuts at:      0     383    1527 
   Size:        383    1144
 
BseYI C'CCAG_C

Cuts at:      0      35    1416    1527 
   Size:         35    1381     111

  Fragments arranged by size: 

                1381     111      35
 
BsiEI CG_ry'CG

Cuts at:      0     402    1527 
   Size:        402    1125
 
BsrDI GCAATG_nn'

Cuts at:      0     929    1527 
   Size:        929     598
 
BsrGI T'GTAC_A

Cuts at:      0     107    1505    1527 
   Size:        107    1398      22

  Fragments arranged by size: 

                1398     107      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     477    1527 
   Size:        477    1050
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1294    1527 
   Size:       1294     233
 
BstYI r'GATC_y

Cuts at:      0      82     163     615    1208    1527 
   Size:         82      81     452     593     319

  Fragments arranged by size: 

                 593     452     319      82      81
 
BtsI GCAGTG_nn'

Cuts at:      0     362    1527 
   Size:        362    1165
 
DraI TTT'AAA

Cuts at:      0     536     903    1527 
   Size:        536     367     624

  Fragments arranged by size: 

                 624     536     367
 
EarI CTCTTCn'nnn_

Cuts at:      0    1010    1527 
   Size:       1010     517
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     290     593    1527 
   Size:        234      56     303     934

  Fragments arranged by size: 

                 934     303     234      56
 
HaeII r_GCGC'y

Cuts at:      0     418    1527 
   Size:        418    1109
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     616     648    1328    1360    1527 
   Size:        616      32     680      32     167

  Fragments arranged by size: 

                 680     616     167      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     141    1516    1527 
   Size:         78      63    1375      11

  Fragments arranged by size: 

                1375      78      63      11
 
MslI CAynn'nnrTG

Cuts at:      0    1292    1527 
   Size:       1292     235
 
NdeI CA'TA_TG

Cuts at:      0    1248    1527 
   Size:       1248     279
 
NspI r_CATG'y

Cuts at:      0    1466    1527 
   Size:       1466      61
 
PstI C_TGCA'G

Cuts at:      0     208     473    1527 
   Size:        208     265    1054

  Fragments arranged by size: 

                1054     265     208
 
PvuI CG_AT'CG

Cuts at:      0     402    1527 
   Size:        402    1125
 
SfcI C'TryA_G

Cuts at:      0     204     469     693    1527 
   Size:        204     265     224     834

  Fragments arranged by size: 

                 834     265     224     204
 
StuI AGG'CCT

Cuts at:      0     275     410    1527 
   Size:        275     135    1117

  Fragments arranged by size: 

                1117     275     135
 
StyI C'CwwG_G

Cuts at:      0    1212    1332    1527 
   Size:       1212     120     195

  Fragments arranged by size: 

                1212     195     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      27     511    1356    1527 
   Size:         27     484     845     171

  Fragments arranged by size: 

                 845     484     171      27
 
TatI w'GTAC_w

Cuts at:      0     107    1505    1527 
   Size:        107    1398      22

  Fragments arranged by size: 

                1398     107      22
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1263    1527 
   Size:       1263     264
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI     AseI    AvrII    BamHI     BbsI    BciVI
    BglII     BpmI     BsaI    BsaAI    BseYI    BsiEI    BsrDI    BsrGI
   BstAPI    BstXI    BstYI     BtsI     DraI     EarI  Eco57MI    HaeII
    Hin4I     MmeI     MslI     NdeI     NspI     PstI     PvuI     SfcI
     StuI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
    AsiSI     AvaI     BaeI     BanI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI