Restriction map for
pFN21AE2767
(Linear) (Six-base) MAPSORT of: KIEE2767.seq Check: 3447 from: 1 to: 5466
KIEE2767.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 779 1375 1750 2703 3162 3524 5466
Size: 779 596 375 953 459 362 1942
Fragments arranged by size:
1942 953 779 596 459 375 362
AccI GT'mk_AC
Cuts at: 0 2561 5466
Size: 2561 2905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 2518 2716 5466
Size: 589 1929 198 2750
Fragments arranged by size:
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AflII C'TTAA_G
Cuts at: 0 1981 5466
Size: 1981 3485
AflIII A'CryG_T
Cuts at: 0 1265 1939 4425 4787 5466
Size: 1265 674 2486 362 679
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 912 2973 5466
Size: 912 2061 2493
Fragments arranged by size:
2493 2061 912
AleI CACnn'nnGTG
Cuts at: 0 928 2647 5466
Size: 928 1719 2819
Fragments arranged by size:
2819 1719 928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 102 787 2636 5466
Size: 102 685 1849 2830
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5437 5466
Size: 5437 29
AlwNI CAG_nnn'CTG
Cuts at: 0 465 2880 2911 4698 5355 5466
Size: 465 2415 31 1787 657 111
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 1122 1242 2081 3270 3806 4247 4310 4622
Size: 1122 120 839 1189 536 441 63 312
Cuts at: 4622 4806 5349 5466
Size: 184 543 117
Fragments arranged by size:
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120 117 63
ApaLI G'TGCA_C
Cuts at: 0 732 1362 2098 4864 5466
Size: 732 630 736 2766 602
Fragments arranged by size:
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AvaI C'yCGr_G
Cuts at: 0 470 1133 1866 2510 2786 4303 4353 5089
Size: 470 663 733 644 276 1517 50 736
Cuts at: 5089 5466
Size: 377
Fragments arranged by size:
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50
BamHI G'GATC_C
Cuts at: 0 1094 5466
Size: 1094 4372
BanI G'GyrC_C
Cuts at: 0 752 817 860 880 918 1915 2068 2445
Size: 752 65 43 20 38 997 153 377
Cuts at: 2445 2667 2835 3091 3382 3531 4233 4408 5466
Size: 222 168 256 291 149 702 175 1058
Fragments arranged by size:
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175 168 153 149 65 43 38 20
BanII G_rGCy'C
Cuts at: 0 385 578 1122 1242 2081 2128 3270 3548
Size: 385 193 544 120 839 47 1142 278
Cuts at: 3548 3603 3806 4130 4187 4247 4310 4423 4622
Size: 55 203 324 57 60 63 113 199
Cuts at: 4622 4806 4879 4960 5252 5318 5349 5466
Size: 184 73 81 292 66 31 117
Fragments arranged by size:
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199 193 184 120 117 113 81 73
66 63 60 57 55 47 31
BbeI G_GCGC'C
Cuts at: 0 756 821 2072 2671 3386 4237 5466
Size: 756 65 1251 599 715 851 1229
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 771 1180 2282 4769 5466
Size: 771 409 1102 2487 697
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 1729 5116 5466
Size: 1729 3387 350
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1649 1683 4497 4531 5466
Size: 1649 34 2814 34 935
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 50 5466
Size: 50 5416
BglI GCCn_nnn'nGGC
Cuts at: 0 1136 2123 2227 2454 2686 3598 3963 4242
Size: 1136 987 104 227 232 912 365 279
Cuts at: 4242 4817 4995 5466
Size: 575 178 471
Fragments arranged by size:
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227 178 104
BglII A'GATC_T
Cuts at: 0 2570 5466
Size: 2570 2896
BlpI GC'TnA_GC
Cuts at: 0 3247 3712 3722 4444 4975 5466
Size: 3247 465 10 722 531 491
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 736 883 923 1122 1242 1366 1565 1918
Size: 736 147 40 199 120 124 199 353
Cuts at: 1918 2081 2102 2450 2840 2903 3270 3536 3659
Size: 163 21 348 390 63 367 266 123
Cuts at: 3659 3806 4247 4310 4433 4583 4622 4806 4868
Size: 147 441 63 123 150 39 184 62
Cuts at: 4868 4929 5132 5349 5466
Size: 61 203 217 117
Fragments arranged by size:
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203 199 199 184 163 150 147 147
124 123 123 120 117 63 63 62
61 40 39 21
BmgBI CAC'GTC
Cuts at: 0 1777 2364 2898 2970 3240 5466
Size: 1777 587 534 72 270 2226
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 94 885 1042 1193 4916 5466
Size: 94 791 157 151 3723 550
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 690 1173 5466
Size: 690 483 4293
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 397 1096 1473 2575 3205 5377 5466
Size: 379 18 699 377 1102 630 2172 89
Fragments arranged by size:
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Bpu10I CC'TnA_GC
Cuts at: 0 478 1729 3404 3602 3923 4417 4954 5116
Size: 478 1251 1675 198 321 494 537 162
Cuts at: 5116 5431 5466
Size: 315 35
Fragments arranged by size:
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162 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1264 1315 2335 2725 5466
Size: 430 834 51 1020 390 2741
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 259 3075 3727 5466
Size: 259 2816 652 1739
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 1251 1266 1940 4166 4428 4788 5466
Size: 1251 15 674 2226 262 360 678
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 753 818 2069 2668 2783 3383 3672 4007
Size: 753 65 1251 599 115 600 289 335
Cuts at: 4007 4226 4234 5466
Size: 219 8 1232
Fragments arranged by size:
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115 65 8
BsaWI w'CCGG_w
Cuts at: 0 647 2575 3326 3839 5466
Size: 647 1928 751 513 1627
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1039 1069 1406 1436 1540 1570 1873 1903
Size: 1039 30 337 30 104 30 303 30
Cuts at: 1903 2573 2603 4639 4669 5466
Size: 670 30 2036 30 797
Fragments arranged by size:
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30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 355 1017 1093 2008 2143 2146 2149
Size: 277 78 662 76 915 135 3 3
Cuts at: 2149 2557 3684 4156 4209 4539 5063 5466
Size: 408 1127 472 53 330 524 403
Fragments arranged by size:
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277 135 78 76 53 3 3
BseYI C'CCAG_C
Cuts at: 0 16 2522 3412 3658 3707 3716 3872 3914
Size: 16 2506 890 246 49 9 156 42
Cuts at: 3914 3953 4087 4134 4820 4979 5232 5348 5466
Size: 39 134 47 686 159 253 116 118
Fragments arranged by size:
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118 116 49 47 42 39 16 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 135 1249 1461 1918 2022 4357 5167
Size: 96 39 1114 212 457 104 2335 810
Cuts at: 5167 5466
Size: 299
Fragments arranged by size:
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39
BsiEI CG_ry'CG
Cuts at: 0 1542 2925 4666 5466
Size: 1542 1383 1741 800
Fragments arranged by size:
1741 1542 1383 800
BsiHKAI G_wGCw'C
Cuts at: 0 736 1250 1366 1537 2102 3548 4187 4322
Size: 736 514 116 171 565 1446 639 135
Cuts at: 4322 4868 4918 5466
Size: 546 50 548
Fragments arranged by size:
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135 116 50
BsiWI C'GTAC_G
Cuts at: 0 2829 5466
Size: 2829 2637
BsmI GAATG_Cn'
Cuts at: 0 1954 5065 5466
Size: 1954 3111 401
Fragments arranged by size:
3111 1954 401
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2960 3696 4098 4551 5466
Size: 2960 736 402 453 915
Fragments arranged by size:
2960 915 736 453 402
Bsp1286I G_dGCh'C
Cuts at: 0 385 578 736 883 923 1122 1242 1250
Size: 385 193 158 147 40 199 120 8
Cuts at: 1250 1366 1537 1565 1918 2081 2102 2128 2450
Size: 116 171 28 353 163 21 26 322
Cuts at: 2450 2840 2903 3270 3536 3548 3603 3659 3806
Size: 390 63 367 266 12 55 56 147
Cuts at: 3806 4130 4187 4247 4310 4322 4423 4433 4583
Size: 324 57 60 63 12 101 10 150
Cuts at: 4583 4622 4806 4868 4879 4918 4929 4960 5132
Size: 39 184 62 11 39 11 31 172
Cuts at: 5132 5252 5318 5349 5466
Size: 120 66 31 117
Fragments arranged by size:
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193 184 172 171 163 158 150 147
147 120 120 117 116 101 66 63
63 62 60 57 56 55 40 39
39 31 31 28 26 21 12 12
11 11 10 8
BspEI T'CCGG_A
Cuts at: 0 647 5466
Size: 647 4819
BspHI T'CATG_A
Cuts at: 0 839 5466
Size: 839 4627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 779 1375 1474 1750 2501 2703 3162 3524
Size: 779 596 99 276 751 202 459 362
Cuts at: 3524 4160 5466
Size: 636 1306
Fragments arranged by size:
1306 779 751 636 596 459 362 276
202 99
BsrBI CCG'CTC
Cuts at: 0 345 669 1100 2169 2675 3927 4016 4490
Size: 345 324 431 1069 506 1252 89 474
Cuts at: 4490 4662 4986 5466
Size: 172 324 480
Fragments arranged by size:
1252 1069 506 480 474 431 345 324
324 172 89
BsrDI GCAATG_nn'
Cuts at: 0 1659 1740 1761 5466
Size: 1659 81 21 3705
Fragments arranged by size:
3705 1659 81 21
BsrFI r'CCGG_y
Cuts at: 0 173 1480 1918 2685 3726 3910 5096 5466
Size: 173 1307 438 767 1041 184 1186 370
Fragments arranged by size:
1307 1186 1041 767 438 370 184 173
BsrGI T'GTAC_A
Cuts at: 0 395 2580 4043 5466
Size: 395 2185 1463 1423
Fragments arranged by size:
2185 1463 1423 395
BssHII G'CGCG_C
Cuts at: 0 3898 5466
Size: 3898 1568
BssSI C'ACGA_G
Cuts at: 0 735 3336 5466
Size: 735 2601 2130
Fragments arranged by size:
2601 2130 735
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2222 2911 5265 5466
Size: 2222 689 2354 201
Fragments arranged by size:
2354 2222 689 201
BstEII G'GTnAC_C
Cuts at: 0 1152 2379 3702 5025 5466
Size: 1152 1227 1323 1323 441
Fragments arranged by size:
1323 1323 1227 1152 441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 1065 2442 2970 4599 5466
Size: 828 237 1377 528 1629 867
Fragments arranged by size:
1629 1377 867 828 528 237
BstYI r'GATC_y
Cuts at: 0 671 1094 2570 3425 5466
Size: 671 423 1476 855 2041
Fragments arranged by size:
2041 1476 855 671 423
Bsu36I CC'TnA_GG
Cuts at: 0 695 2855 5466
Size: 695 2160 2611
Fragments arranged by size:
2611 2160 695
BtgI C'CryG_G
Cuts at: 0 22 206 386 875 1542 1673 2054 3006
Size: 22 184 180 489 667 131 381 952
Cuts at: 3006 3122 3698 4239 4651 4990 5466
Size: 116 576 541 412 339 476
Fragments arranged by size:
952 667 576 541 489 476 412 381
339 184 180 131 116 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4064 5466
Size: 4064 1402
BtsI GCAGTG_nn'
Cuts at: 0 1803 1906 2768 2909 4299 4513 4946 5466
Size: 1803 103 862 141 1390 214 433 520
Fragments arranged by size:
1803 1390 862 520 433 214 141 103
DraIII CAC_nnn'GTG
Cuts at: 0 119 1569 2262 4326 4440 5466
Size: 119 1450 693 2064 114 1026
Fragments arranged by size:
2064 1450 1026 693 119 114
EaeI y'GGCC_r
Cuts at: 0 203 1458 1539 1779 1794 2678 2922 3947
Size: 203 1255 81 240 15 884 244 1025
Cuts at: 3947 4202 4332 4663 4987 5466
Size: 255 130 331 324 479
Fragments arranged by size:
1255 1025 884 479 331 324 255 244
240 203 130 81 15
EagI C'GGCC_G
Cuts at: 0 1539 2922 4663 5466
Size: 1539 1383 1741 803
Fragments arranged by size:
1741 1539 1383 803
EarI CTCTTCn'nnn_
Cuts at: 0 100 5257 5466
Size: 100 5157 209
Fragments arranged by size:
5157 209 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 3217 4890 5466
Size: 208 3009 1673 576
Fragments arranged by size:
3009 1673 576 208
EcoICRI GAG'CTC
Cuts at: 0 3546 4185 5466
Size: 3546 639 1281
Fragments arranged by size:
3546 1281 639
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 397 589 1096 1473 2518 2575 2716
Size: 379 18 192 507 377 1045 57 141
Cuts at: 2716 3205 5377 5466
Size: 489 2172 89
Fragments arranged by size:
2172 1045 507 489 379 377 192 141
89 57 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1513 1876 2554 5466
Size: 1513 363 678 2912
Fragments arranged by size:
2912 1513 678 363
EcoO109I rG'GnC_Cy
Cuts at: 0 71 96 474 1119 1187 1239 1881 2726
Size: 71 25 378 645 68 52 642 845
Cuts at: 2726 3266 3309 3613 3919 4244 4307 4618 5218
Size: 540 43 304 306 325 63 311 600
Cuts at: 5218 5345 5466
Size: 127 121
Fragments arranged by size:
845 645 642 600 540 378 325 311
306 304 127 121 71 68 63 52
43 25
EcoRV GAT'ATC
Cuts at: 0 5010 5466
Size: 5010 456
FspI TGC'GCA
Cuts at: 0 2338 5466
Size: 2338 3128
HaeII r_GCGC'y
Cuts at: 0 180 756 821 2072 2671 3386 4218 4237
Size: 180 576 65 1251 599 715 832 19
Cuts at: 4237 5309 5466
Size: 1072 157
Fragments arranged by size:
1251 1072 832 715 599 576 180 157
65 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 656 688 898 930 1039 1071 1375 1407
Size: 656 32 210 32 109 32 304 32
Cuts at: 1407 1873 1905 2959 2991 3487 3519 4462 4494
Size: 466 32 1054 32 496 32 943 32
Cuts at: 4494 5466
Size: 972
Fragments arranged by size:
1054 972 943 656 496 466 304 210
109 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3201 3780 3825 5466
Size: 3201 579 45 1641
Fragments arranged by size:
3201 1641 579 45
HindIII A'AGCT_T
Cuts at: 0 1003 2698 5466
Size: 1003 1695 2768
Fragments arranged by size:
2768 1695 1003
KasI G'GCGC_C
Cuts at: 0 752 817 2068 2667 3382 4233 5466
Size: 752 65 1251 599 715 851 1233
Fragments arranged by size:
1251 1233 851 752 715 599 65
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 1082 1127 1346 1681 4247 5466
Size: 440 642 45 219 335 2566 1219
Fragments arranged by size:
2566 1219 642 440 335 219 45
MscI TGG'CCA
Cuts at: 0 1460 1781 3949 5466
Size: 1460 321 2168 1517
Fragments arranged by size:
2168 1517 1460 321
MslI CAynn'nnrTG
Cuts at: 0 403 928 1253 2329 2647 2896 3121 5440
Size: 403 525 325 1076 318 249 225 2319
Cuts at: 5440 5466
Size: 26
Fragments arranged by size:
2319 1076 525 403 325 318 249 225
26
MspA1I CmG'CkG
Cuts at: 0 86 208 219 727 1233 1275 1799 2342
Size: 86 122 11 508 506 42 524 543
Cuts at: 2342 2372 2397 2526 2877 3008 3412 3711 4211
Size: 30 25 129 351 131 404 299 500
Cuts at: 4211 4653 4668 4695 4938 4974 5232 5265 5423
Size: 442 15 27 243 36 258 33 158
Cuts at: 5423 5466
Size: 43
Fragments arranged by size:
543 524 508 506 500 442 404 351
299 258 243 158 131 129 122 86
43 42 36 33 30 27 25 15
11
NaeI GCC'GGC
Cuts at: 0 2687 3728 3912 5098 5466
Size: 2687 1041 184 1186 368
Fragments arranged by size:
2687 1186 1041 368 184
NarI GG'CG_CC
Cuts at: 0 753 818 2069 2668 3383 4234 5466
Size: 753 65 1251 599 715 851 1232
Fragments arranged by size:
1251 1232 851 753 715 599 65
NcoI C'CATG_G
Cuts at: 0 22 1673 2054 5466
Size: 22 1651 381 3412
Fragments arranged by size:
3412 1651 381 22
NgoMIV G'CCGG_C
Cuts at: 0 2685 3726 3910 5096 5466
Size: 2685 1041 184 1186 370
Fragments arranged by size:
2685 1186 1041 370 184
NotI GC'GGCC_GC
Cuts at: 0 4663 5466
Size: 4663 803
NspI r_CATG'y
Cuts at: 0 2289 2394 5386 5466
Size: 2289 105 2992 80
Fragments arranged by size:
2992 2289 105 80
PasI CC'CwG_GG
Cuts at: 0 2074 4520 4614 5340 5466
Size: 2074 2446 94 726 126
Fragments arranged by size:
2446 2074 726 126 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 23 774 2538 5355 5466
Size: 23 751 1764 2817 111
Fragments arranged by size:
2817 1764 751 111 23
PfoI T'CCnGG_A
Cuts at: 0 2018 2804 3047 3182 3418 5466
Size: 2018 786 243 135 236 2048
Fragments arranged by size:
2048 2018 786 243 236 135
PmlI CAC'GTG
Cuts at: 0 1251 1266 1940 4166 4428 5466
Size: 1251 15 674 2226 262 1038
Fragments arranged by size:
2226 1251 1038 674 262 15
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1585 1617 3124 3156 5466
Size: 1585 32 1507 32 2310
Fragments arranged by size:
2310 1585 1507 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 71 474 1187 1881 3309 3613 5218 5466
Size: 71 403 713 694 1428 304 1605 248
Fragments arranged by size:
1605 1428 713 694 403 304 248 71
PshAI GACnn'nnGTC
Cuts at: 0 1150 5466
Size: 1150 4316
PspOMI G'GGCC_C
Cuts at: 0 1118 1238 2077 3266 3802 4243 4306 4618
Size: 1118 120 839 1189 536 441 63 312
Cuts at: 4618 4802 5345 5466
Size: 184 543 121
Fragments arranged by size:
1189 1118 839 543 536 441 312 184
121 120 63
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2545 2577 5116 5148 5466
Size: 2545 32 2539 32 318
Fragments arranged by size:
2545 2539 318 32 32
PstI C_TGCA'G
Cuts at: 0 662 722 1046 1405 2775 3173 4673 4973
Size: 662 60 324 359 1370 398 1500 300
Cuts at: 4973 5466
Size: 493
Fragments arranged by size:
1500 1370 662 493 398 359 324 300
60
PvuII CAG'CTG
Cuts at: 0 727 1233 1275 2526 2877 3711 4695 4974
Size: 727 506 42 1251 351 834 984 279
Cuts at: 4974 5232 5466
Size: 258 234
Fragments arranged by size:
1251 984 834 727 506 351 279 258
234 42
SacI G_AGCT'C
Cuts at: 0 3548 4187 5466
Size: 3548 639 1279
Fragments arranged by size:
3548 1279 639
SacII CC_GC'GG
Cuts at: 0 209 3009 4654 5466
Size: 209 2800 1645 812
Fragments arranged by size:
2800 1645 812 209
SanDI GG'GwC_CC
Cuts at: 0 3309 3613 5466
Size: 3309 304 1853
Fragments arranged by size:
3309 1853 304
SapI GCTCTTCn'nnn_
Cuts at: 0 100 5257 5466
Size: 100 5157 209
Fragments arranged by size:
5157 209 100
ScaI AGT'ACT
Cuts at: 0 2203 3481 5466
Size: 2203 1278 1985
Fragments arranged by size:
2203 1985 1278
SexAI A'CCwGG_T
Cuts at: 0 1223 3014 4322 5021 5466
Size: 1223 1791 1308 699 445
Fragments arranged by size:
1791 1308 1223 699 445
SfcI C'TryA_G
Cuts at: 0 658 718 987 1042 1401 2043 2562 2771
Size: 658 60 269 55 359 642 519 209
Cuts at: 2771 3169 3618 4669 4677 4969 5466
Size: 398 449 1051 8 292 497
Fragments arranged by size:
1051 658 642 519 497 449 398 359
292 269 209 60 55 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2686 4995 5466
Size: 2686 2309 471
Fragments arranged by size:
2686 2309 471
SfoI GGC'GCC
Cuts at: 0 754 819 2070 2669 3384 4235 5466
Size: 754 65 1251 599 715 851 1231
Fragments arranged by size:
1251 1231 851 754 715 599 65
SgrAI Cr'CCGG_yG
Cuts at: 0 1918 5466
Size: 1918 3548
SmaI CCC'GGG
Cuts at: 0 1868 2788 4305 4355 5466
Size: 1868 920 1517 50 1111
Fragments arranged by size:
1868 1517 1111 920 50
SmlI C'TyrA_G
Cuts at: 0 445 1243 1330 1981 2350 2740 5466
Size: 445 798 87 651 369 390 2726
Fragments arranged by size:
2726 798 651 445 390 369 87
StuI AGG'CCT
Cuts at: 0 314 558 1084 1139 2221 3157 5466
Size: 314 244 526 55 1082 936 2309
Fragments arranged by size:
2309 1082 936 526 314 244 55
StyI C'CwwG_G
Cuts at: 0 22 74 309 1673 2054 5043 5466
Size: 22 52 235 1364 381 2989 423
Fragments arranged by size:
2989 1364 423 381 235 52 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 781 904 1673 2988 3658 3967 4426
Size: 202 579 123 769 1315 670 309 459
Cuts at: 4426 5311 5452 5466
Size: 885 141 14
Fragments arranged by size:
1315 885 769 670 579 459 309 202
141 123 14
TatI w'GTAC_w
Cuts at: 0 395 893 2201 2580 3479 4043 5276 5466
Size: 395 498 1308 379 899 564 1233 190
Fragments arranged by size:
1308 1233 899 564 498 395 379 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1406 1421 1438 1453 1516 1540 1548 1572
Size: 1406 15 17 15 63 24 8 24
Cuts at: 1572 2190 2222 3477 3509 4639 4671 5466
Size: 618 32 1255 32 1130 32 795
Fragments arranged by size:
1406 1255 1130 795 618 63 32 32
32 24 24 17 15 15 8
Tth111I GACn'n_nGTC
Cuts at: 0 529 1185 2365 5466
Size: 529 656 1180 3101
Fragments arranged by size:
3101 1180 656 529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 1789 3942 5466
Size: 36 1753 2153 1524
Fragments arranged by size:
2153 1753 1524 36
XmaI C'CCGG_G
Cuts at: 0 1866 2786 4303 4353 5466
Size: 1866 920 1517 50 1113
Fragments arranged by size:
1866 1517 1113 920 50
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FspI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuII SacI SacII SanDI SapI ScaI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI ApoI AscI AseI
AsiSI AvrII BaeI BciVI BfrBI BmtI BsaBI BstBI
BstZ17I ClaI CspCI DraI DrdI EcoRI FalI FseI
FspAI HpaI KpnI MfeI MluI NdeI NheI NruI
NsiI PacI PciI PmeI PsiI PspXI PvuI RsrII
SalI SbfI SgfI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmnI ZraI