Restriction map for
pFN21AE2766
(Linear) (Six-base) MAPSORT of: KIEE2766.seq Check: 2352 from: 1 to: 813
KIEE2766.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 592 813
Size: 592 221
AccI GT'mk_AC
Cuts at: 0 171 813
Size: 171 642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 813
Size: 220 593
AlwNI CAG_nnn'CTG
Cuts at: 0 459 813
Size: 459 354
ApaI G_GGCC'C
Cuts at: 0 468 813
Size: 468 345
ApaLI G'TGCA_C
Cuts at: 0 205 603 813
Size: 205 398 210
Fragments arranged by size:
398 210 205
AvaI C'yCGr_G
Cuts at: 0 184 402 739 813
Size: 184 218 337 74
Fragments arranged by size:
337 218 184 74
BanI G'GyrC_C
Cuts at: 0 228 657 813
Size: 228 429 156
Fragments arranged by size:
429 228 156
BanII G_rGCy'C
Cuts at: 0 468 748 813
Size: 468 280 65
Fragments arranged by size:
468 280 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 228 541 606 813
Size: 228 313 65 207
Fragments arranged by size:
313 228 207 65
BglI GCCn_nnn'nGGC
Cuts at: 0 125 813
Size: 125 688
Bme1580I G_kGCm'C
Cuts at: 0 209 231 468 607 662 680 813
Size: 209 22 237 139 55 18 133
Fragments arranged by size:
237 209 139 133 55 22 18
BmgBI CAC'GTC
Cuts at: 0 360 507 813
Size: 360 147 306
Fragments arranged by size:
360 306 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 161 813
Size: 161 652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 603 727 813
Size: 307 296 124 86
Fragments arranged by size:
307 296 124 86
BsaBI GATnn'nnATC
Cuts at: 0 556 813
Size: 556 257
BsaHI Gr'CG_yC
Cuts at: 0 35 796 813
Size: 35 761 17
Fragments arranged by size:
761 35 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 724 813
Size: 649 75 89
Fragments arranged by size:
649 89 75
BseYI C'CCAG_C
Cuts at: 0 224 644 813
Size: 224 420 169
Fragments arranged by size:
420 224 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 743 813
Size: 579 164 70
Fragments arranged by size:
579 164 70
BsiEI CG_ry'CG
Cuts at: 0 14 219 813
Size: 14 205 594
Fragments arranged by size:
594 205 14
BsiHKAI G_wGCw'C
Cuts at: 0 209 422 607 813
Size: 209 213 185 206
Fragments arranged by size:
213 209 206 185
Bsp1286I G_dGCh'C
Cuts at: 0 209 231 422 468 607 662 680 748
Size: 209 22 191 46 139 55 18 68
Cuts at: 748 813
Size: 65
Fragments arranged by size:
209 191 139 68 65 55 46 22
18
BspHI T'CATG_A
Cuts at: 0 695 813
Size: 695 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 592 813
Size: 592 221
BsrBI CCG'CTC
Cuts at: 0 575 813
Size: 575 238
BsrFI r'CCGG_y
Cuts at: 0 31 813
Size: 31 782
BssSI C'ACGA_G
Cuts at: 0 469 813
Size: 469 344
BstAPI GCAn_nnn'nTGC
Cuts at: 0 602 813
Size: 602 211
BstEII G'GTnAC_C
Cuts at: 0 474 813
Size: 474 339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 813
Size: 102 711
BstZ17I GTA'TAC
Cuts at: 0 172 813
Size: 172 641
BtgI C'CryG_G
Cuts at: 0 95 219 305 497 813
Size: 95 124 86 192 316
Fragments arranged by size:
316 192 124 95 86
BtsI GCAGTG_nn'
Cuts at: 0 112 591 813
Size: 112 479 222
Fragments arranged by size:
479 222 112
DraIII CAC_nnn'GTG
Cuts at: 0 602 813
Size: 602 211
EaeI y'GGCC_r
Cuts at: 0 11 29 425 813
Size: 11 18 396 388
Fragments arranged by size:
396 388 18 11
EagI C'GGCC_G
Cuts at: 0 11 813
Size: 11 802
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 644 813
Size: 644 169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 603 727 813
Size: 307 296 124 86
Fragments arranged by size:
307 296 124 86
EcoNI CCTnn'n_nnAGG
Cuts at: 0 528 709 813
Size: 528 181 104
Fragments arranged by size:
528 181 104
EcoO109I rG'GnC_Cy
Cuts at: 0 319 407 464 465 701 813
Size: 319 88 57 1 236 112
Fragments arranged by size:
319 236 112 88 57 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 558 813
Size: 558 255
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 813
Size: 380 433
MscI TGG'CCA
Cuts at: 0 427 813
Size: 427 386
MslI CAynn'nnrTG
Cuts at: 0 271 813
Size: 271 542
MspA1I CmG'CkG
Cuts at: 0 221 813
Size: 221 592
NaeI GCC'GGC
Cuts at: 0 33 813
Size: 33 780
NgoMIV G'CCGG_C
Cuts at: 0 31 813
Size: 31 782
NspI r_CATG'y
Cuts at: 0 183 813
Size: 183 630
PasI CC'CwG_GG
Cuts at: 0 459 813
Size: 459 354
PflMI CCAn_nnn'nTGG
Cuts at: 0 459 813
Size: 459 354
PfoI T'CCnGG_A
Cuts at: 0 143 239 728 813
Size: 143 96 489 85
Fragments arranged by size:
489 143 96 85
PpuMI rG'GwC_Cy
Cuts at: 0 407 701 813
Size: 407 294 112
Fragments arranged by size:
407 294 112
PspOMI G'GGCC_C
Cuts at: 0 464 813
Size: 464 349
SacII CC_GC'GG
Cuts at: 0 222 813
Size: 222 591
SexAI A'CCwGG_T
Cuts at: 0 383 813
Size: 383 430
SfcI C'TryA_G
Cuts at: 0 528 813
Size: 528 285
SmaI CCC'GGG
Cuts at: 0 404 741 813
Size: 404 337 72
Fragments arranged by size:
404 337 72
SphI G_CATG'C
Cuts at: 0 183 813
Size: 183 630
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 221 253 813
Size: 221 32 560
Fragments arranged by size:
560 221 32
XmaI C'CCGG_G
Cuts at: 0 402 739 813
Size: 402 337 74
Fragments arranged by size:
402 337 74
Enzymes that do cut:
AarI AccI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BglI Bme1580I BmgBI BmrI BpmI BsaBI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstZ17I
BtgI BtsI DraIII EaeI EagI EciI Eco57MI EcoNI
EcoO109I FalI MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI SacII SexAI
SfcI SmaI SphI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BsrDI BsrGI
BssHII BstBI BstYI Bsu36I BtgZI ClaI CspCI DraI
DrdI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmnI ZraI