Restriction map for
pFN21AE2765
(Linear) (Six-base) MAPSORT of: KIEE2765.seq Check: 2279 from: 1 to: 1020
KIEE2765.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 187 1020
Size: 187 833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1020
Size: 169 851
AhdI GACnn_n'nnGTC
Cuts at: 0 960 1020
Size: 960 60
AleI CACnn'nnGTG
Cuts at: 0 94 1020
Size: 94 926
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 898 930 1020
Size: 898 32 90
Fragments arranged by size:
898 90 32
AlwNI CAG_nnn'CTG
Cuts at: 0 252 1020
Size: 252 768
ApaLI G'TGCA_C
Cuts at: 0 943 1020
Size: 943 77
AvaI C'yCGr_G
Cuts at: 0 441 1020
Size: 441 579
BanI G'GyrC_C
Cuts at: 0 369 513 618 670 890 1020
Size: 369 144 105 52 220 130
Fragments arranged by size:
369 220 144 130 105 52
BanII G_rGCy'C
Cuts at: 0 137 1020
Size: 137 883
BbeI G_GCGC'C
Cuts at: 0 517 1020
Size: 517 503
BbsI GAAGACnn'nnnn_
Cuts at: 0 452 1020
Size: 452 568
BbvCI CC'TCA_GC
Cuts at: 0 304 1020
Size: 304 716
BclI T'GATC_A
Cuts at: 0 158 1020
Size: 158 862
BfrBI ATG'CAT
Cuts at: 0 834 1020
Size: 834 186
BglI GCCn_nnn'nGGC
Cuts at: 0 982 1020
Size: 982 38
Bme1580I G_kGCm'C
Cuts at: 0 623 673 788 947 1020
Size: 623 50 115 159 73
Fragments arranged by size:
623 159 115 73 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 909 1020
Size: 909 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 295 415 1020
Size: 151 144 120 605
Fragments arranged by size:
605 151 144 120
Bpu10I CC'TnA_GC
Cuts at: 0 304 1020
Size: 304 716
BsaBI GATnn'nnATC
Cuts at: 0 733 798 1020
Size: 733 65 222
Fragments arranged by size:
733 222 65
BsaHI Gr'CG_yC
Cuts at: 0 514 1020
Size: 514 506
BsaWI w'CCGG_w
Cuts at: 0 1001 1020
Size: 1001 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 587 617 1020
Size: 388 30 169 30 403
Fragments arranged by size:
403 388 169 30 30
BseYI C'CCAG_C
Cuts at: 0 591 744 1020
Size: 591 153 276
Fragments arranged by size:
591 276 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 1020
Size: 165 855
BsiHKAI G_wGCw'C
Cuts at: 0 35 50 137 947 1020
Size: 35 15 87 810 73
Fragments arranged by size:
810 87 73 35 15
Bsp1286I G_dGCh'C
Cuts at: 0 35 50 137 623 673 788 947 1020
Size: 35 15 87 486 50 115 159 73
Fragments arranged by size:
486 159 115 87 73 50 35 15
BspEI T'CCGG_A
Cuts at: 0 1001 1020
Size: 1001 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 187 1020
Size: 187 833
BsrFI r'CCGG_y
Cuts at: 0 416 805 1020
Size: 416 389 215
Fragments arranged by size:
416 389 215
BssSI C'ACGA_G
Cuts at: 0 43 1020
Size: 43 977
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 252 1020
Size: 188 64 768
Fragments arranged by size:
768 188 64
BstEII G'GTnAC_C
Cuts at: 0 3 1020
Size: 3 1017
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 639 1020
Size: 123 516 381
Fragments arranged by size:
516 381 123
BstYI r'GATC_y
Cuts at: 0 728 1004 1020
Size: 728 276 16
Fragments arranged by size:
728 276 16
Bsu36I CC'TnA_GG
Cuts at: 0 451 499 1020
Size: 451 48 521
Fragments arranged by size:
521 451 48
BtgI C'CryG_G
Cuts at: 0 92 518 665 917 1020
Size: 92 426 147 252 103
Fragments arranged by size:
426 252 147 103 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 857 1020
Size: 857 163
BtsI GCAGTG_nn'
Cuts at: 0 186 1020
Size: 186 834
EaeI y'GGCC_r
Cuts at: 0 98 974 1020
Size: 98 876 46
Fragments arranged by size:
876 98 46
EarI CTCTTCn'nnn_
Cuts at: 0 10 55 145 394 1020
Size: 10 45 90 249 626
Fragments arranged by size:
626 249 90 45 10
EcoICRI GAG'CTC
Cuts at: 0 135 1020
Size: 135 885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 169 295 415 1020
Size: 151 18 126 120 605
Fragments arranged by size:
605 151 126 120 18
EcoO109I rG'GnC_Cy
Cuts at: 0 20 1020
Size: 20 1000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 780 1020
Size: 780 240
HaeII r_GCGC'y
Cuts at: 0 423 517 659 1020
Size: 423 94 142 361
Fragments arranged by size:
423 361 142 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 468 500 587 619 946 978
Size: 388 32 48 32 87 32 327 32
Cuts at: 978 1020
Size: 42
Fragments arranged by size:
388 327 87 48 42 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 513 1020
Size: 513 507
MscI TGG'CCA
Cuts at: 0 100 1020
Size: 100 920
MslI CAynn'nnrTG
Cuts at: 0 94 1020
Size: 94 926
MspA1I CmG'CkG
Cuts at: 0 252 315 919 1020
Size: 252 63 604 101
Fragments arranged by size:
604 252 101 63
NaeI GCC'GGC
Cuts at: 0 418 1020
Size: 418 602
NarI GG'CG_CC
Cuts at: 0 514 1020
Size: 514 506
NcoI C'CATG_G
Cuts at: 0 92 1020
Size: 92 928
NgoMIV G'CCGG_C
Cuts at: 0 416 1020
Size: 416 604
NsiI A_TGCA'T
Cuts at: 0 836 1020
Size: 836 184
NspI r_CATG'y
Cuts at: 0 838 1020
Size: 838 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 93 107 564 1020
Size: 93 14 457 456
Fragments arranged by size:
457 456 93 14
PfoI T'CCnGG_A
Cuts at: 0 128 584 1020
Size: 128 456 436
Fragments arranged by size:
456 436 128
PpuMI rG'GwC_Cy
Cuts at: 0 20 1020
Size: 20 1000
SacI G_AGCT'C
Cuts at: 0 137 1020
Size: 137 883
SacII CC_GC'GG
Cuts at: 0 920 1020
Size: 920 100
SapI GCTCTTCn'nnn_
Cuts at: 0 55 1020
Size: 55 965
SexAI A'CCwGG_T
Cuts at: 0 106 1020
Size: 106 914
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 982 1020
Size: 982 38
SfoI GGC'GCC
Cuts at: 0 515 1020
Size: 515 505
StuI AGG'CCT
Cuts at: 0 171 1020
Size: 171 849
StyI C'CwwG_G
Cuts at: 0 92 980 1020
Size: 92 888 40
Fragments arranged by size:
888 92 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 571 636 758 807 1020
Size: 199 372 65 122 49 213
Fragments arranged by size:
372 213 199 122 65 49
Tth111I GACn'n_nGTC
Cuts at: 0 493 1020
Size: 493 527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 561 1020
Size: 561 459
Enzymes that do cut:
AarI AcuI AhdI AleI AloI AlwNI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BclI BfrBI BglI
Bme1580I BmrI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PflMI PfoI PpuMI SacI SacII SapI
SexAI SfiI SfoI StuI StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaAI BseRI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI XbaI
XhoI XmaI XmnI ZraI