Restriction map for
pFN21AE2760
(Linear) (Six-base) MAPSORT of: KIEE2760.seq Check: 2404 from: 1 to: 753
KIEE2760.orf
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 753
Size: 720 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 222 253 753
Size: 222 31 500
Fragments arranged by size:
500 222 31
ApoI r'AATT_y
Cuts at: 0 316 454 517 753
Size: 316 138 63 236
Fragments arranged by size:
316 236 138 63
BanII G_rGCy'C
Cuts at: 0 429 693 753
Size: 429 264 60
Fragments arranged by size:
429 264 60
BciVI GTATCCnnnnn_n'
Cuts at: 0 285 753
Size: 285 468
BfrBI ATG'CAT
Cuts at: 0 535 753
Size: 535 218
BglII A'GATC_T
Cuts at: 0 658 753
Size: 658 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 753
Size: 387 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 753
Size: 707 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 416 446 753
Size: 19 30 367 30 307
Fragments arranged by size:
367 307 30 30 19
BseYI C'CCAG_C
Cuts at: 0 467 753
Size: 467 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 753
Size: 526 227
BsiEI CG_ry'CG
Cuts at: 0 599 753
Size: 599 154
BsiHKAI G_wGCw'C
Cuts at: 0 332 753
Size: 332 421
BsmI GAATG_Cn'
Cuts at: 0 535 753
Size: 535 218
Bsp1286I G_dGCh'C
Cuts at: 0 332 429 693 753
Size: 332 97 264 60
Fragments arranged by size:
332 264 97 60
BsrBI CCG'CTC
Cuts at: 0 30 134 753
Size: 30 104 619
Fragments arranged by size:
619 104 30
BsrGI T'GTAC_A
Cuts at: 0 482 753
Size: 482 271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 504 753
Size: 504 249
BstYI r'GATC_y
Cuts at: 0 658 753
Size: 658 95
DrdI GACnn_nn'nnGTC
Cuts at: 0 157 753
Size: 157 596
EaeI y'GGCC_r
Cuts at: 0 311 596 753
Size: 311 285 157
Fragments arranged by size:
311 285 157
EagI C'GGCC_G
Cuts at: 0 596 753
Size: 596 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 345 753
Size: 345 408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 720 753
Size: 707 13 33
Fragments arranged by size:
707 33 13
EcoRI G'AATT_C
Cuts at: 0 316 753
Size: 316 437
HaeII r_GCGC'y
Cuts at: 0 403 753
Size: 403 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 753
Size: 3 32 718
Fragments arranged by size:
718 32 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 625 753
Size: 67 558 128
Fragments arranged by size:
558 128 67
MslI CAynn'nnrTG
Cuts at: 0 335 753
Size: 335 418
MspA1I CmG'CkG
Cuts at: 0 237 753
Size: 237 516
NsiI A_TGCA'T
Cuts at: 0 537 753
Size: 537 216
PfoI T'CCnGG_A
Cuts at: 0 277 684 753
Size: 277 407 69
Fragments arranged by size:
407 277 69
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 753
Size: 62 32 659
Fragments arranged by size:
659 62 32
PstI C_TGCA'G
Cuts at: 0 409 753
Size: 409 344
PvuII CAG'CTG
Cuts at: 0 237 753
Size: 237 516
SexAI A'CCwGG_T
Cuts at: 0 265 625 753
Size: 265 360 128
Fragments arranged by size:
360 265 128
SfcI C'TryA_G
Cuts at: 0 405 662 753
Size: 405 257 91
Fragments arranged by size:
405 257 91
StyI C'CwwG_G
Cuts at: 0 508 753
Size: 508 245
TatI w'GTAC_w
Cuts at: 0 482 753
Size: 482 271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 753
Size: 416 32 305
Fragments arranged by size:
416 305 32
Enzymes that do cut:
AcuI AlfI ApoI BanII BciVI BfrBI BglII BmrI
BpmI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BsrBI BsrGI BstAPI BstYI DrdI EaeI EagI EciI
Eco57MI EcoRI HaeII Hin4I MmeI MslI MspA1I NsiI
PfoI PpiI PstI PvuII SexAI SfcI StyI TatI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII EarI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI