Restriction map for
pFN21AE2759
(Linear) (Six-base) MAPSORT of: KIEE2759.seq Check: 4519 from: 1 to: 2346
KIEE2759.orf
With 182 enzymes: SgfI *
September 1, 2019 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1107 1547 1788 2346
Size: 1107 440 241 558
Fragments arranged by size:
1107 558 440 241
AccI GT'mk_AC
Cuts at: 0 1058 1883 1931 2346
Size: 1058 825 48 415
Fragments arranged by size:
1058 825 415 48
Acc65I G'GTAC_C
Cuts at: 0 734 2346
Size: 734 1612
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 913 1068 1225 1471 1587 1683 2307
Size: 499 414 155 157 246 116 96 624
Cuts at: 2307 2346
Size: 39
Fragments arranged by size:
624 499 414 246 157 155 116 96
39
AflIII A'CryG_T
Cuts at: 0 506 896 2346
Size: 506 390 1450
Fragments arranged by size:
1450 506 390
AleI CACnn'nnGTG
Cuts at: 0 802 1129 2341 2346
Size: 802 327 1212 5
Fragments arranged by size:
1212 802 327 5
AlwNI CAG_nnn'CTG
Cuts at: 0 74 168 1406 1469 1565 1803 2226 2259
Size: 74 94 1238 63 96 238 423 33
Cuts at: 2259 2346
Size: 87
Fragments arranged by size:
1238 423 238 96 94 87 74 63
33
ApaLI G'TGCA_C
Cuts at: 0 1153 2346
Size: 1153 1193
AvaI C'yCGr_G
Cuts at: 0 1348 1544 1552 1874 2346
Size: 1348 196 8 322 472
Fragments arranged by size:
1348 472 322 196 8
BamHI G'GATC_C
Cuts at: 0 23 236 2346
Size: 23 213 2110
Fragments arranged by size:
2110 213 23
BanI G'GyrC_C
Cuts at: 0 603 734 1535 1680 1754 1761 2346
Size: 603 131 801 145 74 7 585
Fragments arranged by size:
801 603 585 145 131 74 7
BanII G_rGCy'C
Cuts at: 0 38 179 1149 1559 2014 2346
Size: 38 141 970 410 455 332
Fragments arranged by size:
970 455 410 332 141 38
BbeI G_GCGC'C
Cuts at: 0 1684 1758 2346
Size: 1684 74 588
Fragments arranged by size:
1684 588 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 490 1962 2346
Size: 219 271 1472 384
Fragments arranged by size:
1472 384 271 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1455 1489 2346
Size: 1455 34 857
Fragments arranged by size:
1455 857 34
BglI GCCn_nnn'nGGC
Cuts at: 0 294 316 1370 1943 2346
Size: 294 22 1054 573 403
Fragments arranged by size:
1054 573 403 294 22
BglII A'GATC_T
Cuts at: 0 680 848 2346
Size: 680 168 1498
Fragments arranged by size:
1498 680 168
BlpI GC'TnA_GC
Cuts at: 0 1462 2346
Size: 1462 884
Bme1580I G_kGCm'C
Cuts at: 0 1103 1157 1538 2174 2346
Size: 1103 54 381 636 172
Fragments arranged by size:
1103 636 381 172 54
BmgBI CAC'GTC
Cuts at: 0 488 1047 2343 2346
Size: 488 559 1296 3
Fragments arranged by size:
1296 559 488 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 894 2346
Size: 894 1452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 304 865 1129 1825 2227 2346
Size: 190 114 561 264 696 402 119
Fragments arranged by size:
696 561 402 264 190 119 114
Bpu10I CC'TnA_GC
Cuts at: 0 178 1558 2346
Size: 178 1380 788
Fragments arranged by size:
1380 788 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1924 2346
Size: 1924 422
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 131 400 1801 2346
Size: 73 58 269 1401 545
Fragments arranged by size:
1401 545 269 73 58
BsaAI yAC'GTr
Cuts at: 0 897 2133 2346
Size: 897 1236 213
Fragments arranged by size:
1236 897 213
BsaBI GATnn'nnATC
Cuts at: 0 64 1435 1633 2346
Size: 64 1371 198 713
Fragments arranged by size:
1371 713 198 64
BsaHI Gr'CG_yC
Cuts at: 0 474 1681 1727 1755 2346
Size: 474 1207 46 28 591
Fragments arranged by size:
1207 591 474 46 28
BsaWI w'CCGG_w
Cuts at: 0 20 2346
Size: 20 2326
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 958 988 2056 2086 2200 2230 2346
Size: 958 30 1068 30 114 30 116
Fragments arranged by size:
1068 958 116 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 268 1126 2346
Size: 187 81 858 1220
Fragments arranged by size:
1220 858 187 81
BseYI C'CCAG_C
Cuts at: 0 579 2259 2310 2346
Size: 579 1680 51 36
Fragments arranged by size:
1680 579 51 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 741 865 1447 2052 2346
Size: 442 299 124 582 605 294
Fragments arranged by size:
605 582 442 299 294 124
BsiEI CG_ry'CG
Cuts at: 0 611 1097 1130 2346
Size: 611 486 33 1216
Fragments arranged by size:
1216 611 486 33
BsiHKAI G_wGCw'C
Cuts at: 0 179 1157 1559 2132 2338 2346
Size: 179 978 402 573 206 8
Fragments arranged by size:
978 573 402 206 179 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1307 2346
Size: 1307 1039
Bsp1286I G_dGCh'C
Cuts at: 0 38 179 1103 1149 1157 1538 1559 2014
Size: 38 141 924 46 8 381 21 455
Cuts at: 2014 2132 2174 2338 2346
Size: 118 42 164 8
Fragments arranged by size:
924 455 381 164 141 118 46 42
38 21 8 8
BspEI T'CCGG_A
Cuts at: 0 20 2346
Size: 20 2326
BspHI T'CATG_A
Cuts at: 0 380 1016 1028 1175 2346
Size: 380 636 12 147 1171
Fragments arranged by size:
1171 636 380 147 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 763 1107 1244 1547 1788 2346
Size: 722 41 344 137 303 241 558
Fragments arranged by size:
722 558 344 303 241 137 41
BsrBI CCG'CTC
Cuts at: 0 641 1511 2346
Size: 641 870 835
Fragments arranged by size:
870 835 641
BsrDI GCAATG_nn'
Cuts at: 0 1829 1847 2346
Size: 1829 18 499
Fragments arranged by size:
1829 499 18
BsrFI r'CCGG_y
Cuts at: 0 606 2346
Size: 606 1740
BssHII G'CGCG_C
Cuts at: 0 864 2346
Size: 864 1482
BssSI C'ACGA_G
Cuts at: 0 112 345 1708 2346
Size: 112 233 1363 638
Fragments arranged by size:
1363 638 233 112
BstEII G'GTnAC_C
Cuts at: 0 339 1119 1137 1494 2346
Size: 339 780 18 357 852
Fragments arranged by size:
852 780 357 339 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 819 1131 1365 1443 1662 1737 1842 2106
Size: 819 312 234 78 219 75 105 264
Cuts at: 2106 2346
Size: 240
Fragments arranged by size:
819 312 264 240 234 219 105 78
75
BstYI r'GATC_y
Cuts at: 0 23 236 417 680 848 1715 2346
Size: 23 213 181 263 168 867 631
Fragments arranged by size:
867 631 263 213 181 168 23
Bsu36I CC'TnA_GG
Cuts at: 0 290 2346
Size: 290 2056
BtgI C'CryG_G
Cuts at: 0 833 1124 1130 1489 2346
Size: 833 291 6 359 857
Fragments arranged by size:
857 833 359 291 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1299 2139 2346
Size: 1299 840 207
Fragments arranged by size:
1299 840 207
BtsI GCAGTG_nn'
Cuts at: 0 103 1276 2346
Size: 103 1173 1070
Fragments arranged by size:
1173 1070 103
DraIII CAC_nnn'GTG
Cuts at: 0 1161 2346
Size: 1161 1185
DrdI GACnn_nn'nnGTC
Cuts at: 0 253 2346
Size: 253 2093
EaeI y'GGCC_r
Cuts at: 0 317 608 961 1094 1127 1341 1433 1664
Size: 317 291 353 133 33 214 92 231
Cuts at: 1664 2096 2346
Size: 432 250
Fragments arranged by size:
432 353 317 291 250 231 214 133
92 33
EagI C'GGCC_G
Cuts at: 0 608 1094 1127 2346
Size: 608 486 33 1219
Fragments arranged by size:
1219 608 486 33
EarI CTCTTCn'nnn_
Cuts at: 0 5 1087 1382 1426 1702 2346
Size: 5 1082 295 44 276 644
Fragments arranged by size:
1082 644 295 276 44 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1708 2092 2346
Size: 1708 384 254
Fragments arranged by size:
1708 384 254
EcoICRI GAG'CTC
Cuts at: 0 177 1557 2346
Size: 177 1380 789
Fragments arranged by size:
1380 789 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 304 499 865 913 1068 1129 1225
Size: 190 114 195 366 48 155 61 96
Cuts at: 1225 1471 1587 1683 1825 2227 2307 2346
Size: 246 116 96 142 402 80 39
Fragments arranged by size:
402 366 246 195 190 155 142 116
114 96 96 80 61 48 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 460 2346
Size: 460 1886
EcoO109I rG'GnC_Cy
Cuts at: 0 295 888 1232 1548 1787 1992 2346
Size: 295 593 344 316 239 205 354
Fragments arranged by size:
593 354 344 316 295 239 205
HaeII r_GCGC'y
Cuts at: 0 1684 1758 2346
Size: 1684 74 588
Fragments arranged by size:
1684 588 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1131 1163 2346
Size: 1131 32 1183
Fragments arranged by size:
1183 1131 32
HincII GTy'rAC
Cuts at: 0 1596 1932 1953 2346
Size: 1596 336 21 393
Fragments arranged by size:
1596 393 336 21
HindIII A'AGCT_T
Cuts at: 0 1207 2346
Size: 1207 1139
KasI G'GCGC_C
Cuts at: 0 1680 1754 2346
Size: 1680 74 592
Fragments arranged by size:
1680 592 74
KpnI G_GTAC'C
Cuts at: 0 738 2346
Size: 738 1608
MscI TGG'CCA
Cuts at: 0 1435 2098 2346
Size: 1435 663 248
Fragments arranged by size:
1435 663 248
MslI CAynn'nnrTG
Cuts at: 0 202 595 601 703 802 817 1129 1174
Size: 202 393 6 102 99 15 312 45
Cuts at: 1174 1441 1627 1660 1735 1816 2341 2346
Size: 267 186 33 75 81 525 5
Fragments arranged by size:
525 393 312 267 202 186 102 99
81 75 45 33 15 6 5
MspA1I CmG'CkG
Cuts at: 0 57 741 1403 1677 1686 1752 2346
Size: 57 684 662 274 9 66 594
Fragments arranged by size:
684 662 594 274 66 57 9
NaeI GCC'GGC
Cuts at: 0 608 2346
Size: 608 1738
NarI GG'CG_CC
Cuts at: 0 1681 1755 2346
Size: 1681 74 591
Fragments arranged by size:
1681 591 74
NgoMIV G'CCGG_C
Cuts at: 0 606 2346
Size: 606 1740
NspI r_CATG'y
Cuts at: 0 510 600 2346
Size: 510 90 1746
Fragments arranged by size:
1746 510 90
PciI A'CATG_T
Cuts at: 0 506 2346
Size: 506 1840
PflMI CCAn_nnn'nTGG
Cuts at: 0 1050 2346
Size: 1050 1296
PfoI T'CCnGG_A
Cuts at: 0 167 209 281 512 842 1106 1988 2346
Size: 167 42 72 231 330 264 882 358
Fragments arranged by size:
882 358 330 264 231 167 72 42
PmlI CAC'GTG
Cuts at: 0 2133 2346
Size: 2133 213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 1240 1272 2346
Size: 433 32 775 32 1074
Fragments arranged by size:
1074 775 433 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 888 1232 1548 1787 1992 2346
Size: 888 344 316 239 205 354
Fragments arranged by size:
888 354 344 316 239 205
PstI C_TGCA'G
Cuts at: 0 1283 1799 2346
Size: 1283 516 547
Fragments arranged by size:
1283 547 516
SacI G_AGCT'C
Cuts at: 0 179 1559 2346
Size: 179 1380 787
Fragments arranged by size:
1380 787 179
SalI G'TCGA_C
Cuts at: 0 1930 2346
Size: 1930 416
SanDI GG'GwC_CC
Cuts at: 0 1548 2346
Size: 1548 798
SapI GCTCTTCn'nnn_
Cuts at: 0 1087 1702 2346
Size: 1087 615 644
Fragments arranged by size:
1087 644 615
SexAI A'CCwGG_T
Cuts at: 0 1481 2346
Size: 1481 865
SfcI C'TryA_G
Cuts at: 0 1279 1795 1896 2346
Size: 1279 516 101 450
Fragments arranged by size:
1279 516 450 101
SfoI GGC'GCC
Cuts at: 0 1682 1756 2346
Size: 1682 74 590
Fragments arranged by size:
1682 590 74
SmaI CCC'GGG
Cuts at: 0 1350 1546 2346
Size: 1350 196 800
Fragments arranged by size:
1350 800 196
SmlI C'TyrA_G
Cuts at: 0 1939 2346
Size: 1939 407
StuI AGG'CCT
Cuts at: 0 88 2346
Size: 88 2258
StyI C'CwwG_G
Cuts at: 0 1775 1979 2346
Size: 1775 204 367
Fragments arranged by size:
1775 367 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 145 790 1306 1482 2251 2346
Size: 97 48 645 516 176 769 95
Fragments arranged by size:
769 645 516 176 97 95 48
TatI w'GTAC_w
Cuts at: 0 566 2346
Size: 566 1780
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1948 1980 2346
Size: 1948 32 366
Fragments arranged by size:
1948 366 32
Tth111I GACn'n_nGTC
Cuts at: 0 106 433 2346
Size: 106 327 1913
Fragments arranged by size:
1913 327 106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 1131 1659 1818 1996 2346
Size: 840 291 528 159 178 350
Fragments arranged by size:
840 528 350 291 178 159
XmaI C'CCGG_G
Cuts at: 0 1348 1544 2346
Size: 1348 196 802
Fragments arranged by size:
1348 802 196
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BcgI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII KasI KpnI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmlI PpiI
PpuMI PstI SacI SalI SanDI SapI SexAI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BfrBI BmtI BplI BsiWI BsmI BsrGI
BstAPI BstBI BstZ17I ClaI CspCI DraI EcoRI EcoRV
FalI FseI FspI FspAI HpaI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI
ZraI