(Linear) (Six-base) MAPSORT of: KIEE2753.seq  Check: 7114  from: 1  to: 207



KIEE2753.orf

 With 182 enzymes: SgfI * 

                        June 30, 2019 14:46  ..

AfeI AGC'GCT

Cuts at:      0     151     207 
   Size:        151      56
 
BanII G_rGCy'C

Cuts at:      0     173     207 
   Size:        173      34
 
BglII A'GATC_T

Cuts at:      0      11     207 
   Size:         11     196
 
BsaBI GATnn'nnATC

Cuts at:      0      58     207 
   Size:         58     149
 
BsaHI Gr'CG_yC

Cuts at:      0      32     207 
   Size:         32     175
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     181     207 
   Size:        181      26
 
Bsp1286I G_dGCh'C

Cuts at:      0     173     207 
   Size:        173      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     200     207 
   Size:        200       7
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      14     207 
   Size:         14     193
 
BstYI r'GATC_y

Cuts at:      0      11      59     207 
   Size:         11      48     148

  Fragments arranged by size: 

                 148      48      11
 
BtgI C'CryG_G

Cuts at:      0     161     207 
   Size:        161      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     189     207 
   Size:        189      18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     123     207 
   Size:        123      84
 
HaeII r_GCGC'y

Cuts at:      0     153     207 
   Size:        153      54
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     207 
   Size:         87     120
 
MspA1I CmG'CkG

Cuts at:      0      42     207 
   Size:         42     165
 
NcoI C'CATG_G

Cuts at:      0     161     207 
   Size:        161      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     101     207 
   Size:        101     106
 
PpuMI rG'GwC_Cy

Cuts at:      0     189     207 
   Size:        189      18
 
StyI C'CwwG_G

Cuts at:      0      68     161     207 
   Size:         68      93      46

  Fragments arranged by size: 

                  93      68      46
 

 Enzymes that do cut:

     AfeI    BanII    BglII    BsaBI    BsaHI    BseRI Bsp1286I    BspMI
   BstAPI    BstYI     BtgI EcoO109I     FalI    HaeII     MmeI   MspA1I
     NcoI    PflMI    PpuMI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI