Restriction map for
pFN21AE2748
(Linear) (Six-base) MAPSORT of: KIEE2748.seq Check: 433 from: 1 to: 7395
KIEE2748.orf
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 6069 7395
Size: 6069 1326
AccI GT'mk_AC
Cuts at: 0 210 429 5246 7026 7395
Size: 210 219 4817 1780 369
Fragments arranged by size:
4817 1780 369 219 210
AclI AA'CG_TT
Cuts at: 0 3051 7395
Size: 3051 4344
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 956 1176 1345 1769 1958 3301 3957
Size: 683 273 220 169 424 189 1343 656
Cuts at: 3957 4240 4741 4820 4861 5336 5689 5884 6785
Size: 283 501 79 41 475 353 195 901
Cuts at: 6785 7395
Size: 610
Fragments arranged by size:
1343 901 683 656 610 501 475 424
353 283 273 220 195 189 169 79
41
AflII C'TTAA_G
Cuts at: 0 2520 4747 7395
Size: 2520 2227 2648
Fragments arranged by size:
2648 2520 2227
AflIII A'CryG_T
Cuts at: 0 4101 4903 7395
Size: 4101 802 2492
Fragments arranged by size:
4101 2492 802
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2040 3131 4747 7395
Size: 2040 1091 1616 2648
Fragments arranged by size:
2648 2040 1616 1091
AlwNI CAG_nnn'CTG
Cuts at: 0 1527 1604 2379 3648 4764 5617 7188 7395
Size: 1527 77 775 1269 1116 853 1571 207
Fragments arranged by size:
1571 1527 1269 1116 853 775 207 77
ApaI G_GGCC'C
Cuts at: 0 5449 6809 7395
Size: 5449 1360 586
Fragments arranged by size:
5449 1360 586
ApaLI G'TGCA_C
Cuts at: 0 6239 7395
Size: 6239 1156
ApoI r'AATT_y
Cuts at: 0 78 2169 2605 3111 4361 4472 4711 5064
Size: 78 2091 436 506 1250 111 239 353
Cuts at: 5064 5732 6907 7039 7395
Size: 668 1175 132 356
Fragments arranged by size:
2091 1250 1175 668 506 436 356 353
239 132 111 78
AseI AT'TA_AT
Cuts at: 0 4694 5396 7395
Size: 4694 702 1999
Fragments arranged by size:
4694 1999 702
AvaI C'yCGr_G
Cuts at: 0 3330 7395
Size: 3330 4065
BamHI G'GATC_C
Cuts at: 0 5208 6348 7395
Size: 5208 1140 1047
Fragments arranged by size:
5208 1140 1047
BanI G'GyrC_C
Cuts at: 0 6636 7395
Size: 6636 759
BanII G_rGCy'C
Cuts at: 0 5449 6809 7395
Size: 5449 1360 586
Fragments arranged by size:
5449 1360 586
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 864 1715 2005 2605 5517 5797 7112
Size: 556 308 851 290 600 2912 280 1315
Cuts at: 7112 7209 7395
Size: 97 186
Fragments arranged by size:
2912 1315 851 600 556 308 290 280
186 97
BbvCI CC'TCA_GC
Cuts at: 0 2294 2660 7395
Size: 2294 366 4735
Fragments arranged by size:
4735 2294 366
BclI T'GATC_A
Cuts at: 0 797 5300 7395
Size: 797 4503 2095
Fragments arranged by size:
4503 2095 797
BfrBI ATG'CAT
Cuts at: 0 868 4231 6109 6207 7395
Size: 868 3363 1878 98 1188
Fragments arranged by size:
3363 1878 1188 868 98
BglII A'GATC_T
Cuts at: 0 303 2823 7395
Size: 303 2520 4572
Fragments arranged by size:
4572 2520 303
BlpI GC'TnA_GC
Cuts at: 0 4518 4892 6280 7395
Size: 4518 374 1388 1115
Fragments arranged by size:
4518 1388 1115 374
Bme1580I G_kGCm'C
Cuts at: 0 552 5449 5744 6221 6243 6809 7145 7395
Size: 552 4897 295 477 22 566 336 250
Fragments arranged by size:
4897 566 552 477 336 295 250 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 1515 2275 2492 6347 7395
Size: 1515 760 217 3855 1048
Fragments arranged by size:
3855 1515 1048 760 217
BmtI G_CTAG'C
Cuts at: 0 1652 7395
Size: 1652 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 75 1394 1546 1676 2356 3681 4037
Size: 37 38 1319 152 130 680 1325 356
Cuts at: 4037 4102 5798 6844 7327 7395
Size: 65 1696 1046 483 68
Fragments arranged by size:
1696 1325 1319 1046 680 483 356 152
130 68 65 38 37
Bpu10I CC'TnA_GC
Cuts at: 0 287 832 1091 1618 2294 2660 7395
Size: 287 545 259 527 676 366 4735
Fragments arranged by size:
4735 676 545 527 366 287 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 2256 3384 5439 6638 7395
Size: 696 1560 1128 2055 1199 757
Fragments arranged by size:
2055 1560 1199 1128 757 696
BsaI GGTCTCn'nnnn_
Cuts at: 0 5888 7395
Size: 5888 1507
BsaAI yAC'GTr
Cuts at: 0 602 7053 7395
Size: 602 6451 342
Fragments arranged by size:
6451 602 342
BsaBI GATnn'nnATC
Cuts at: 0 802 3287 3560 7395
Size: 802 2485 273 3835
Fragments arranged by size:
3835 2485 802 273
BsaHI Gr'CG_yC
Cuts at: 0 5764 7395
Size: 5764 1631
BsaWI w'CCGG_w
Cuts at: 0 789 4277 4340 7395
Size: 789 3488 63 3055
Fragments arranged by size:
3488 3055 789 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2354 2384 3224 3254 3780 3810 4091 4121
Size: 2354 30 840 30 526 30 281 30
Cuts at: 4121 6817 6847 7395
Size: 2696 30 548
Fragments arranged by size:
2696 2354 840 548 526 281 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3100 3699 4229 7395
Size: 3100 599 530 3166
Fragments arranged by size:
3166 3100 599 530
BseYI C'CCAG_C
Cuts at: 0 3352 5759 7395
Size: 3352 2407 1636
Fragments arranged by size:
3352 2407 1636
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 2374 2495 3570 4409 4946 5079 7146
Size: 724 1650 121 1075 839 537 133 2067
Cuts at: 7146 7395
Size: 249
Fragments arranged by size:
2067 1650 1075 839 724 537 249 133
121
BsiEI CG_ry'CG
Cuts at: 0 790 7395
Size: 790 6605
BsiHKAI G_wGCw'C
Cuts at: 0 2272 4109 4617 6243 7208 7395
Size: 2272 1837 508 1626 965 187
Fragments arranged by size:
2272 1837 1626 965 508 187
BsmI GAATG_Cn'
Cuts at: 0 538 2391 3399 3621 7395
Size: 538 1853 1008 222 3774
Fragments arranged by size:
3774 1853 1008 538 222
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3568 7395
Size: 3568 3827
Bsp1286I G_dGCh'C
Cuts at: 0 552 2272 4109 4617 5449 5744 6221 6243
Size: 552 1720 1837 508 832 295 477 22
Cuts at: 6243 6809 7145 7208 7395
Size: 566 336 63 187
Fragments arranged by size:
1837 1720 832 566 552 508 477 336
295 187 63 22
BspEI T'CCGG_A
Cuts at: 0 4340 7395
Size: 4340 3055
BspHI T'CATG_A
Cuts at: 0 1734 3744 5603 7395
Size: 1734 2010 1859 1792
Fragments arranged by size:
2010 1859 1792 1734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4633 6069 7395
Size: 4633 1436 1326
Fragments arranged by size:
4633 1436 1326
BsrBI CCG'CTC
Cuts at: 0 6177 7395
Size: 6177 1218
BsrDI GCAATG_nn'
Cuts at: 0 370 998 1567 2024 2288 2986 3132 5486
Size: 370 628 569 457 264 698 146 2354
Cuts at: 5486 6071 6928 7395
Size: 585 857 467
Fragments arranged by size:
2354 857 698 628 585 569 467 457
370 264 146
BsrGI T'GTAC_A
Cuts at: 0 723 3039 4352 6741 7395
Size: 723 2316 1313 2389 654
Fragments arranged by size:
2389 2316 1313 723 654
BssSI C'ACGA_G
Cuts at: 0 2972 6452 7395
Size: 2972 3480 943
Fragments arranged by size:
3480 2972 943
BstAPI GCAn_nnn'nTGC
Cuts at: 0 349 3105 5935 6247 7149 7188 7395
Size: 349 2756 2830 312 902 39 207
Fragments arranged by size:
2830 2756 902 349 312 207 39
BstBI TT'CG_AA
Cuts at: 0 1286 7395
Size: 1286 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5751 6012 7395
Size: 5751 261 1383
Fragments arranged by size:
5751 1383 261
BstYI r'GATC_y
Cuts at: 0 303 803 1047 1170 1613 1933 2127 2823
Size: 303 500 244 123 443 320 194 696
Cuts at: 2823 3561 4057 5208 6348 7395
Size: 738 496 1151 1140 1047
Fragments arranged by size:
1151 1140 1047 738 696 500 496 443
320 303 244 194 123
BstZ17I GTA'TAC
Cuts at: 0 7027 7395
Size: 7027 368
BtgI C'CryG_G
Cuts at: 0 2474 3182 7395
Size: 2474 708 4213
Fragments arranged by size:
4213 2474 708
BtsI GCAGTG_nn'
Cuts at: 0 669 1345 2029 3261 3558 4934 5280 5317
Size: 669 676 684 1232 297 1376 346 37
Cuts at: 5317 6467 6567 6773 7395
Size: 1150 100 206 622
Fragments arranged by size:
1376 1232 1150 684 676 669 622 346
297 206 100 37
DraI TTT'AAA
Cuts at: 0 620 5645 7044 7395
Size: 620 5025 1399 351
Fragments arranged by size:
5025 1399 620 351
DraIII CAC_nnn'GTG
Cuts at: 0 5275 7395
Size: 5275 2120
DrdI GACnn_nn'nnGTC
Cuts at: 0 3788 7395
Size: 3788 3607
EaeI y'GGCC_r
Cuts at: 0 2471 4268 7395
Size: 2471 1797 3127
Fragments arranged by size:
3127 2471 1797
EarI CTCTTCn'nnn_
Cuts at: 0 5 341 757 1022 2263 2532 2668 3566
Size: 5 336 416 265 1241 269 136 898
Cuts at: 3566 3746 3756 4492 4796 4880 7346 7395
Size: 180 10 736 304 84 2466 49
Fragments arranged by size:
2466 1241 898 736 416 336 304 269
265 180 136 84 49 10 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 75 683 956 1176 1345 1394 1546
Size: 37 38 608 273 220 169 49 152
Cuts at: 1546 1676 1769 1958 2356 3301 3681 3957 4037
Size: 130 93 189 398 945 380 276 80
Cuts at: 4037 4102 4240 4741 4820 4861 5336 5689 5798
Size: 65 138 501 79 41 475 353 109
Cuts at: 5798 5884 6785 6844 7327 7395
Size: 86 901 59 483 68
Fragments arranged by size:
945 901 608 501 483 475 398 380
353 276 273 220 189 169 152 138
130 109 93 86 80 79 68 65
59 49 41 38 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1094 1621 2569 7395
Size: 1094 527 948 4826
Fragments arranged by size:
4826 1094 948 527
EcoO109I rG'GnC_Cy
Cuts at: 0 907 1099 5446 6805 6806 7395
Size: 907 192 4347 1359 1 589
Fragments arranged by size:
4347 1359 907 589 192 1
EcoRI G'AATT_C
Cuts at: 0 5732 7395
Size: 5732 1663
EcoRV GAT'ATC
Cuts at: 0 113 7395
Size: 113 7282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 664 2146 3919 4600 5584 7395
Size: 664 1482 1773 681 984 1811
Fragments arranged by size:
1811 1773 1482 984 681 664
FspI TGC'GCA
Cuts at: 0 7171 7395
Size: 7171 224
HaeII r_GCGC'y
Cuts at: 0 250 7395
Size: 250 7145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 381 413 1542 1574 2549 2581
Size: 97 32 252 32 1129 32 975 32
Cuts at: 2581 3224 3256 4540 4572 5148 5180 6040 6072
Size: 643 32 1284 32 576 32 860 32
Cuts at: 6072 6254 6286 7395
Size: 182 32 1109
Fragments arranged by size:
1284 1129 1109 975 860 643 576 252
182 97 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2753 7395
Size: 2753 4642
HindIII A'AGCT_T
Cuts at: 0 628 6329 7395
Size: 628 5701 1066
Fragments arranged by size:
5701 1066 628
HpaI GTT'AAC
Cuts at: 0 2753 7395
Size: 2753 4642
MfeI C'AATT_G
Cuts at: 0 59 1889 5930 5939 7395
Size: 59 1830 4041 9 1456
Fragments arranged by size:
4041 1830 1456 59 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1148 1418 2067 5186 5236 7395
Size: 485 663 270 649 3119 50 2159
Fragments arranged by size:
3119 2159 663 649 485 270 50
MscI TGG'CCA
Cuts at: 0 2473 4270 7395
Size: 2473 1797 3125
Fragments arranged by size:
3125 2473 1797
MslI CAynn'nnrTG
Cuts at: 0 358 1039 3677 4318 4456 5455 5496 7395
Size: 358 681 2638 641 138 999 41 1899
Fragments arranged by size:
2638 1899 999 681 641 358 138 41
MspA1I CmG'CkG
Cuts at: 0 1404 1879 6276 6876 7395
Size: 1404 475 4397 600 519
Fragments arranged by size:
4397 1404 600 519 475
NcoI C'CATG_G
Cuts at: 0 2474 3182 7395
Size: 2474 708 4213
Fragments arranged by size:
4213 2474 708
NdeI CA'TA_TG
Cuts at: 0 258 5297 7395
Size: 258 5039 2098
Fragments arranged by size:
5039 2098 258
NheI G'CTAG_C
Cuts at: 0 1648 7395
Size: 1648 5747
NsiI A_TGCA'T
Cuts at: 0 870 4233 6111 6209 7395
Size: 870 3363 1878 98 1186
Fragments arranged by size:
3363 1878 1186 870 98
NspI r_CATG'y
Cuts at: 0 946 1354 2353 2620 2791 4105 4392 4455
Size: 946 408 999 267 171 1314 287 63
Cuts at: 4455 4891 4907 4917 4961 5703 7395
Size: 436 16 10 44 742 1692
Fragments arranged by size:
1692 1314 999 946 742 436 408 287
267 171 63 44 16 10
PciI A'CATG_T
Cuts at: 0 4101 4903 7395
Size: 4101 802 2492
Fragments arranged by size:
4101 2492 802
PflMI CCAn_nnn'nTGG
Cuts at: 0 98 993 5235 7395
Size: 98 895 4242 2160
Fragments arranged by size:
4242 2160 895 98
PfoI T'CCnGG_A
Cuts at: 0 3078 3697 4053 4135 7395
Size: 3078 619 356 82 3260
Fragments arranged by size:
3260 3078 619 356 82
PpuMI rG'GwC_Cy
Cuts at: 0 907 1099 7395
Size: 907 192 6296
Fragments arranged by size:
6296 907 192
PshAI GACnn'nnGTC
Cuts at: 0 5539 7395
Size: 5539 1856
PsiI TTA'TAA
Cuts at: 0 2740 7395
Size: 2740 4655
PspOMI G'GGCC_C
Cuts at: 0 5445 6805 7395
Size: 5445 1360 590
Fragments arranged by size:
5445 1360 590
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3621 3653 7395
Size: 3621 32 3742
Fragments arranged by size:
3742 3621 32
PstI C_TGCA'G
Cuts at: 0 3124 3663 5027 5553 7395
Size: 3124 539 1364 526 1842
Fragments arranged by size:
3124 1842 1364 539 526
PvuII CAG'CTG
Cuts at: 0 1404 1879 6276 7395
Size: 1404 475 4397 1119
Fragments arranged by size:
4397 1404 1119 475
SapI GCTCTTCn'nnn_
Cuts at: 0 5 2263 7395
Size: 5 2258 5132
Fragments arranged by size:
5132 2258 5
ScaI AGT'ACT
Cuts at: 0 481 696 7395
Size: 481 215 6699
Fragments arranged by size:
6699 481 215
SexAI A'CCwGG_T
Cuts at: 0 225 423 2447 4652 7395
Size: 225 198 2024 2205 2743
Fragments arranged by size:
2743 2205 2024 225 198
SfcI C'TryA_G
Cuts at: 0 430 543 2111 3120 3659 4187 5023 5549
Size: 430 113 1568 1009 539 528 836 526
Cuts at: 5549 6159 7395
Size: 610 1236
Fragments arranged by size:
1568 1236 1009 836 610 539 528 526
430 113
SmlI C'TyrA_G
Cuts at: 0 711 2271 2520 3363 4747 5418 6653 7395
Size: 711 1560 249 843 1384 671 1235 742
Fragments arranged by size:
1560 1384 1235 843 742 711 671 249
SpeI A'CTAG_T
Cuts at: 0 2436 4216 7395
Size: 2436 1780 3179
Fragments arranged by size:
3179 2436 1780
SphI G_CATG'C
Cuts at: 0 4891 5703 7395
Size: 4891 812 1692
Fragments arranged by size:
4891 1692 812
SspI AAT'ATT
Cuts at: 0 6201 7395
Size: 6201 1194
StuI AGG'CCT
Cuts at: 0 5121 7395
Size: 5121 2274
StyI C'CwwG_G
Cuts at: 0 2474 3182 3419 5537 5666 7395
Size: 2474 708 237 2118 129 1729
Fragments arranged by size:
2474 2118 1729 708 237 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1847 4985 7395
Size: 1847 3138 2410
Fragments arranged by size:
3138 2410 1847
TatI w'GTAC_w
Cuts at: 0 479 694 717 723 3030 3039 3138 4352
Size: 479 215 23 6 2307 9 99 1214
Cuts at: 4352 5087 6302 6741 7395
Size: 735 1215 439 654
Fragments arranged by size:
2307 1215 1214 735 654 479 439 215
99 23 9 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2354 2386 3201 3233 7395
Size: 2354 32 815 32 4162
Fragments arranged by size:
4162 2354 815 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2463 7395
Size: 2463 4932
XbaI T'CTAG_A
Cuts at: 0 1110 3778 4773 7395
Size: 1110 2668 995 2622
Fragments arranged by size:
2668 2622 1110 995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2647 7395
Size: 2647 4748
XmnI GAAnn'nnTTC
Cuts at: 0 1539 3314 4528 5585 6944 7395
Size: 1539 1775 1214 1057 1359 451
Fragments arranged by size:
1775 1539 1359 1214 1057 451
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtsI
DraI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI
NheI NsiI NspI PciI PflMI PfoI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuII SapI ScaI SexAI
SfcI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI AleI AloI AscI
AsiSI AvrII BaeI BbeI BcgI BciVI BglI BmgBI
BplI BsiWI BsrFI BssHII BstEII Bsu36I BtgZI ClaI
CspCI EagI EciI EcoICRI FseI FspAI KasI KpnI
MluI NaeI NarI NgoMIV NotI NruI PacI PasI
PmeI PmlI PpiI PspXI PvuI RsrII SacI SacII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI SwaI XhoI XmaI ZraI