Restriction map for
pFN21AE2745
(Linear) (Six-base) MAPSORT of: KIEE2745.seq Check: 5929 from: 1 to: 1161
KIEE2745.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 896 1161
Size: 838 58 265
Fragments arranged by size:
838 265 58
AleI CACnn'nnGTG
Cuts at: 0 124 1078 1161
Size: 124 954 83
Fragments arranged by size:
954 124 83
AlwNI CAG_nnn'CTG
Cuts at: 0 213 1161
Size: 213 948
ApaI G_GGCC'C
Cuts at: 0 486 680 1100 1161
Size: 486 194 420 61
Fragments arranged by size:
486 420 194 61
ApoI r'AATT_y
Cuts at: 0 23 1161
Size: 23 1138
AvaI C'yCGr_G
Cuts at: 0 632 1121 1161
Size: 632 489 40
Fragments arranged by size:
632 489 40
BanII G_rGCy'C
Cuts at: 0 143 275 448 486 496 680 874 971
Size: 143 132 173 38 10 184 194 97
Cuts at: 971 991 1100 1161
Size: 20 109 61
Fragments arranged by size:
194 184 173 143 132 109 97 61
38 20 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 617 1161
Size: 617 544
BbvCI CC'TCA_GC
Cuts at: 0 349 1161
Size: 349 812
BclI T'GATC_A
Cuts at: 0 555 1161
Size: 555 606
BlpI GC'TnA_GC
Cuts at: 0 490 1161
Size: 490 671
Bme1580I G_kGCm'C
Cuts at: 0 486 680 1100 1161
Size: 486 194 420 61
Fragments arranged by size:
486 420 194 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 674 1161
Size: 674 487
Bpu10I CC'TnA_GC
Cuts at: 0 269 349 1161
Size: 269 80 812
Fragments arranged by size:
812 269 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1161
Size: 652 509
BsaI GGTCTCn'nnnn_
Cuts at: 0 634 1063 1161
Size: 634 429 98
Fragments arranged by size:
634 429 98
BsaWI w'CCGG_w
Cuts at: 0 47 743 971 1161
Size: 47 696 228 190
Fragments arranged by size:
696 228 190 47
BseYI C'CCAG_C
Cuts at: 0 278 441 485 495 624 903 1161
Size: 278 163 44 10 129 279 258
Fragments arranged by size:
279 278 258 163 129 44 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 367 647 1161
Size: 339 28 280 514
Fragments arranged by size:
514 339 280 28
BsiEI CG_ry'CG
Cuts at: 0 1021 1161
Size: 1021 140
BsiHKAI G_wGCw'C
Cuts at: 0 143 275 971 1161
Size: 143 132 696 190
Fragments arranged by size:
696 190 143 132
BsmI GAATG_Cn'
Cuts at: 0 407 1161
Size: 407 754
Bsp1286I G_dGCh'C
Cuts at: 0 143 275 448 486 496 680 874 971
Size: 143 132 173 38 10 184 194 97
Cuts at: 971 991 1100 1161
Size: 20 109 61
Fragments arranged by size:
194 184 173 143 132 109 97 61
38 20 10
BspEI T'CCGG_A
Cuts at: 0 743 971 1161
Size: 743 228 190
Fragments arranged by size:
743 228 190
BspHI T'CATG_A
Cuts at: 0 173 1161
Size: 173 988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 377 1161
Size: 272 105 784
Fragments arranged by size:
784 272 105
BsrBI CCG'CTC
Cuts at: 0 100 1161
Size: 100 1061
BsrFI r'CCGG_y
Cuts at: 0 503 1161
Size: 503 658
BstAPI GCAn_nnn'nTGC
Cuts at: 0 516 1161
Size: 516 645
Bsu36I CC'TnA_GG
Cuts at: 0 578 773 1161
Size: 578 195 388
Fragments arranged by size:
578 388 195
BtgI C'CryG_G
Cuts at: 0 119 1161
Size: 119 1042
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 720 1161
Size: 720 441
BtsI GCAGTG_nn'
Cuts at: 0 379 898 1065 1161
Size: 379 519 167 96
Fragments arranged by size:
519 379 167 96
DraIII CAC_nnn'GTG
Cuts at: 0 114 1006 1161
Size: 114 892 155
Fragments arranged by size:
892 155 114
EaeI y'GGCC_r
Cuts at: 0 191 1018 1161
Size: 191 827 143
Fragments arranged by size:
827 191 143
EagI C'GGCC_G
Cuts at: 0 1018 1161
Size: 1018 143
EarI CTCTTCn'nnn_
Cuts at: 0 757 872 1161
Size: 757 115 289
Fragments arranged by size:
757 289 115
EcoICRI GAG'CTC
Cuts at: 0 141 273 969 1161
Size: 141 132 696 192
Fragments arranged by size:
696 192 141 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 896 1161
Size: 838 58 265
Fragments arranged by size:
838 265 58
EcoO109I rG'GnC_Cy
Cuts at: 0 366 482 565 676 1096 1097 1161
Size: 366 116 83 111 420 1 64
Fragments arranged by size:
420 366 116 111 83 64 1
EcoRI G'AATT_C
Cuts at: 0 23 1161
Size: 23 1138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1161
Size: 773 388
MscI TGG'CCA
Cuts at: 0 193 1161
Size: 193 968
MslI CAynn'nnrTG
Cuts at: 0 124 322 920 1078 1161
Size: 124 198 598 158 83
Fragments arranged by size:
598 198 158 124 83
MspA1I CmG'CkG
Cuts at: 0 85 353 489 1161
Size: 85 268 136 672
Fragments arranged by size:
672 268 136 85
NaeI GCC'GGC
Cuts at: 0 505 1161
Size: 505 656
NgoMIV G'CCGG_C
Cuts at: 0 503 1161
Size: 503 658
NspI r_CATG'y
Cuts at: 0 519 1087 1161
Size: 519 568 74
Fragments arranged by size:
568 519 74
PfoI T'CCnGG_A
Cuts at: 0 362 1161
Size: 362 799
PpuMI rG'GwC_Cy
Cuts at: 0 366 565 1161
Size: 366 199 596
Fragments arranged by size:
596 366 199
PspOMI G'GGCC_C
Cuts at: 0 482 676 1096 1161
Size: 482 194 420 65
Fragments arranged by size:
482 420 194 65
PstI C_TGCA'G
Cuts at: 0 218 1161
Size: 218 943
PvuII CAG'CTG
Cuts at: 0 85 353 489 1161
Size: 85 268 136 672
Fragments arranged by size:
672 268 136 85
SacI G_AGCT'C
Cuts at: 0 143 275 971 1161
Size: 143 132 696 190
Fragments arranged by size:
696 190 143 132
SanDI GG'GwC_CC
Cuts at: 0 565 1161
Size: 565 596
SfcI C'TryA_G
Cuts at: 0 214 1161
Size: 214 947
SgrAI Cr'CCGG_yG
Cuts at: 0 503 1161
Size: 503 658
SmaI CCC'GGG
Cuts at: 0 634 1123 1161
Size: 634 489 38
Fragments arranged by size:
634 489 38
SmlI C'TyrA_G
Cuts at: 0 667 1161
Size: 667 494
SphI G_CATG'C
Cuts at: 0 1087 1161
Size: 1087 74
StyI C'CwwG_G
Cuts at: 0 392 1161
Size: 392 769
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 550 1161
Size: 550 611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 100 132 1161
Size: 100 32 1029
Fragments arranged by size:
1029 100 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 1108 1161
Size: 438 670 53
Fragments arranged by size:
670 438 53
XmaI C'CCGG_G
Cuts at: 0 632 1121 1161
Size: 632 489 40
Fragments arranged by size:
632 489 40
Enzymes that do cut:
AcuI AleI AlwNI ApaI ApoI AvaI BanII BbsI
BbvCI BclI BlpI Bme1580I BmrI Bpu10I BpuEI BsaI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BstAPI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRI MmeI MscI MslI MspA1I NaeI NgoMIV NspI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SfcI
SgrAI SmaI SmlI SphI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI
BglI BglII BmgBI BmtI BplI BpmI BsaAI BsaBI
BsaHI BsaXI BseRI BsiWI BsmBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI
DraI DrdI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SnaBI
SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI