Restriction map for
pFN21AE2744
(Linear) (Six-base) MAPSORT of: KIEE2744.seq Check: 4865 from: 1 to: 645
KIEE2744.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AatII G_ACGT'C
Cuts at: 0 302 645
Size: 302 343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 645
Size: 298 347
AlwNI CAG_nnn'CTG
Cuts at: 0 602 645
Size: 602 43
BamHI G'GATC_C
Cuts at: 0 119 645
Size: 119 526
BbsI GAAGACnn'nnnn_
Cuts at: 0 313 645
Size: 313 332
BbvCI CC'TCA_GC
Cuts at: 0 178 645
Size: 178 467
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 233 267 645
Size: 233 34 378
Fragments arranged by size:
378 233 34
BglI GCCn_nnn'nGGC
Cuts at: 0 97 645
Size: 97 548
BglII A'GATC_T
Cuts at: 0 205 645
Size: 205 440
Bpu10I CC'TnA_GC
Cuts at: 0 178 272 645
Size: 178 94 373
Fragments arranged by size:
373 178 94
BsaAI yAC'GTr
Cuts at: 0 563 645
Size: 563 82
BsaHI Gr'CG_yC
Cuts at: 0 299 645
Size: 299 346
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 645
Size: 496 149
BsiHKAI G_wGCw'C
Cuts at: 0 628 645
Size: 628 17
Bsp1286I G_dGCh'C
Cuts at: 0 628 645
Size: 628 17
BstYI r'GATC_y
Cuts at: 0 119 205 645
Size: 119 86 440
Fragments arranged by size:
440 119 86
BtgI C'CryG_G
Cuts at: 0 86 234 576 645
Size: 86 148 342 69
Fragments arranged by size:
342 148 86 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 399 645
Size: 399 246
DraIII CAC_nnn'GTG
Cuts at: 0 81 645
Size: 81 564
EaeI y'GGCC_r
Cuts at: 0 83 461 645
Size: 83 378 184
Fragments arranged by size:
378 184 83
EarI CTCTTCn'nnn_
Cuts at: 0 253 645
Size: 253 392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 645
Size: 298 347
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 463 645
Size: 331 132 182
Fragments arranged by size:
331 182 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 387 645
Size: 264 123 258
Fragments arranged by size:
264 258 123
FspI TGC'GCA
Cuts at: 0 341 645
Size: 341 304
FspAI rTGC'GCAy
Cuts at: 0 341 645
Size: 341 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 171 203 645
Size: 171 32 442
Fragments arranged by size:
442 171 32
HincII GTy'rAC
Cuts at: 0 36 645
Size: 36 609
HindIII A'AGCT_T
Cuts at: 0 280 645
Size: 280 365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 645
Size: 137 508
MscI TGG'CCA
Cuts at: 0 85 463 645
Size: 85 378 182
Fragments arranged by size:
378 182 85
MspA1I CmG'CkG
Cuts at: 0 265 645
Size: 265 380
NcoI C'CATG_G
Cuts at: 0 86 234 576 645
Size: 86 148 342 69
Fragments arranged by size:
342 148 86 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 235 329 645
Size: 235 94 316
Fragments arranged by size:
316 235 94
PfoI T'CCnGG_A
Cuts at: 0 347 645
Size: 347 298
PstI C_TGCA'G
Cuts at: 0 107 264 645
Size: 107 157 381
Fragments arranged by size:
381 157 107
PvuII CAG'CTG
Cuts at: 0 265 645
Size: 265 380
SexAI A'CCwGG_T
Cuts at: 0 5 645
Size: 5 640
SfcI C'TryA_G
Cuts at: 0 103 260 645
Size: 103 157 385
Fragments arranged by size:
385 157 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 97 645
Size: 97 548
StyI C'CwwG_G
Cuts at: 0 86 133 234 464 512 519 576 590
Size: 86 47 101 230 48 7 57 14
Cuts at: 590 645
Size: 55
Fragments arranged by size:
230 101 86 57 55 48 47 14
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 634 645
Size: 634 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 645
Size: 93 552
XmnI GAAnn'nnTTC
Cuts at: 0 310 645
Size: 310 335
ZraI GAC'GTC
Cuts at: 0 300 645
Size: 300 345
Enzymes that do cut:
AatII AcuI AlwNI BamHI BbsI BbvCI BcgI BglI
BglII Bpu10I BsaAI BsaHI BsgI BsiHKAI Bsp1286I BstYI
BtgI BtgZI DraIII EaeI EarI Eco57MI EcoNI FalI
FspI FspAI Hin4I HincII HindIII MmeI MscI MspA1I
NcoI PflMI PfoI PstI PvuII SexAI SfcI SfiI
StyI TaqII XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI
HaeII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI