Restriction map for
pFN21AE2741
(Linear) (Six-base) MAPSORT of: KIEE2741.seq Check: 4103 from: 1 to: 1077
KIEE2741.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AatII G_ACGT'C
Cuts at: 0 584 1077
Size: 584 493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 992 1077
Size: 992 85
AlwNI CAG_nnn'CTG
Cuts at: 0 840 1077
Size: 840 237
ApoI r'AATT_y
Cuts at: 0 175 358 899 1077
Size: 175 183 541 178
Fragments arranged by size:
541 183 178 175
AvaI C'yCGr_G
Cuts at: 0 25 1077
Size: 25 1052
BanI G'GyrC_C
Cuts at: 0 910 1077
Size: 910 167
BanII G_rGCy'C
Cuts at: 0 823 956 1077
Size: 823 133 121
Fragments arranged by size:
823 133 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 241 980 1077
Size: 241 739 97
Fragments arranged by size:
739 241 97
BglI GCCn_nnn'nGGC
Cuts at: 0 682 1077
Size: 682 395
Bme1580I G_kGCm'C
Cuts at: 0 816 1077
Size: 816 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 34 283 422 450 553 781 788
Size: 28 6 249 139 28 103 228 7
Cuts at: 788 1077
Size: 289
Fragments arranged by size:
289 249 228 139 103 28 28 7
6
BsaHI Gr'CG_yC
Cuts at: 0 581 1077
Size: 581 496
BsaWI w'CCGG_w
Cuts at: 0 903 1077
Size: 903 174
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 322 1077
Size: 322 755
BseYI C'CCAG_C
Cuts at: 0 32 870 1077
Size: 32 838 207
Fragments arranged by size:
838 207 32
BsiHKAI G_wGCw'C
Cuts at: 0 251 1077
Size: 251 826
BsiWI C'GTAC_G
Cuts at: 0 239 1077
Size: 239 838
BsmI GAATG_Cn'
Cuts at: 0 222 1077
Size: 222 855
Bsp1286I G_dGCh'C
Cuts at: 0 251 816 823 956 1077
Size: 251 565 7 133 121
Fragments arranged by size:
565 251 133 121 7
BspEI T'CCGG_A
Cuts at: 0 903 1077
Size: 903 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 596 1077
Size: 596 481
BsrBI CCG'CTC
Cuts at: 0 567 1077
Size: 567 510
BsrDI GCAATG_nn'
Cuts at: 0 62 867 1077
Size: 62 805 210
Fragments arranged by size:
805 210 62
BstAPI GCAn_nnn'nTGC
Cuts at: 0 840 1077
Size: 840 237
BstXI CCAn_nnnn'nTGG
Cuts at: 0 702 1077
Size: 702 375
BstYI r'GATC_y
Cuts at: 0 317 397 1077
Size: 317 80 680
Fragments arranged by size:
680 317 80
Bsu36I CC'TnA_GG
Cuts at: 0 575 1077
Size: 575 502
BtsI GCAGTG_nn'
Cuts at: 0 326 553 686 1077
Size: 326 227 133 391
Fragments arranged by size:
391 326 227 133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 117 152 1077
Size: 117 35 925
Fragments arranged by size:
925 117 35
EaeI y'GGCC_r
Cuts at: 0 75 125 674 776 887 1077
Size: 75 50 549 102 111 190
Fragments arranged by size:
549 190 111 102 75 50
EarI CTCTTCn'nnn_
Cuts at: 0 157 1013 1077
Size: 157 856 64
Fragments arranged by size:
856 157 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 34 283 422 450 553 781 788
Size: 28 6 249 139 28 103 228 7
Cuts at: 788 992 1077
Size: 204 85
Fragments arranged by size:
249 228 204 139 103 85 28 28
7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 201 1077
Size: 201 876
EcoO109I rG'GnC_Cy
Cuts at: 0 1002 1077
Size: 1002 75
EcoRI G'AATT_C
Cuts at: 0 175 358 1077
Size: 175 183 719
Fragments arranged by size:
719 183 175
EcoRV GAT'ATC
Cuts at: 0 668 1077
Size: 668 409
HaeII r_GCGC'y
Cuts at: 0 33 1077
Size: 33 1044
HincII GTy'rAC
Cuts at: 0 585 1077
Size: 585 492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 683 1077
Size: 375 308 394
Fragments arranged by size:
394 375 308
MscI TGG'CCA
Cuts at: 0 127 889 1077
Size: 127 762 188
Fragments arranged by size:
762 188 127
MslI CAynn'nnrTG
Cuts at: 0 1033 1077
Size: 1033 44
MspA1I CmG'CkG
Cuts at: 0 6 837 1077
Size: 6 831 240
Fragments arranged by size:
831 240 6
NspI r_CATG'y
Cuts at: 0 933 1077
Size: 933 144
PasI CC'CwG_GG
Cuts at: 0 816 1077
Size: 816 261
PpuMI rG'GwC_Cy
Cuts at: 0 1002 1077
Size: 1002 75
PstI C_TGCA'G
Cuts at: 0 296 1077
Size: 296 781
PvuII CAG'CTG
Cuts at: 0 837 1077
Size: 837 240
SfcI C'TryA_G
Cuts at: 0 292 1077
Size: 292 785
SmaI CCC'GGG
Cuts at: 0 27 1077
Size: 27 1050
StyI C'CwwG_G
Cuts at: 0 128 197 695 1077
Size: 128 69 498 382
Fragments arranged by size:
498 382 128 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 937 1077
Size: 24 913 140
Fragments arranged by size:
913 140 24
TatI w'GTAC_w
Cuts at: 0 281 1077
Size: 281 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 726 758 1077
Size: 726 32 319
Fragments arranged by size:
726 319 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 921 1077
Size: 921 156
XmaI C'CCGG_G
Cuts at: 0 25 1077
Size: 25 1052
ZraI GAC'GTC
Cuts at: 0 582 1077
Size: 582 495
Enzymes that do cut:
AatII AcuI AlwNI ApoI AvaI BanI BanII BbsI
BglI Bme1580I BpmI BsaHI BsaWI BseRI BseYI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BstAPI
BstXI BstYI Bsu36I BtsI CspCI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI EcoRV HaeII HincII MmeI MscI
MslI MspA1I NspI PasI PpuMI PstI PvuII SfcI
SmaI StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsgI BsiEI
BsmBI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgI BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoICRI FalI FseI FspI FspAI Hin4I HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmnI